Multi-sensory Gamma Stimulation Ameliorates Alzheimer’s-Associated Pathology and Improves Cognition

Cell, Год журнала: 2019, Номер 177(2), С. 256 - 271.e22

Опубликована: Март 14, 2019

Язык: Английский

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Tissue clearing and its applications in neuroscience

Nature reviews. Neuroscience, Год журнала: 2020, Номер 21(2), С. 61 - 79

Опубликована: Янв. 2, 2020

Язык: Английский

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Self-organization and symmetry breaking in intestinal organoid development

Nature, Год журнала: 2019, Номер 569(7754), С. 66 - 72

Опубликована: Апрель 24, 2019

Язык: Английский

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Multiplexed and scalable super-resolution imaging of three-dimensional protein localization in size-adjustable tissues

Nature Biotechnology, Год журнала: 2016, Номер 34(9), С. 973 - 981

Опубликована: Июль 25, 2016

Язык: Английский

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Panoptic imaging of transparent mice reveals whole-body neuronal projections and skull–meninges connections

Nature Neuroscience, Год журнала: 2018, Номер 22(2), С. 317 - 327

Опубликована: Дек. 20, 2018

Язык: Английский

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Protection of tissue physicochemical properties using polyfunctional crosslinkers

Nature Biotechnology, Год журнала: 2018, Номер 37(1), С. 73 - 83

Опубликована: Дек. 17, 2018

Язык: Английский

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Bioinspired neuron-like electronics

Nature Materials, Год журнала: 2019, Номер 18(5), С. 510 - 517

Опубликована: Фев. 25, 2019

Язык: Английский

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Self‐organized developmental patterning and differentiation in cerebral organoids

The EMBO Journal, Год журнала: 2017, Номер 36(10), С. 1316 - 1329

Опубликована: Март 10, 2017

Язык: Английский

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Tissue clearing of both hard and soft tissue organs with the PEGASOS method

Cell Research, Год журнала: 2018, Номер 28(8), С. 803 - 818

Опубликована: Май 29, 2018

Tissue clearing technique enables visualization of opaque organs and tissues in 3-dimensions (3-D) by turning tissue transparent. Current methods are restricted limited types that can be cleared with each individual protocol, which inevitably led to the presence blind-spots within whole body or parts imaging. Hard including bones teeth still most difficult cleared. In addition, loss endogenous fluorescence remains a major concern for solvent-based methods. Here, we developed polyethylene glycol (PEG)-associated solvent system (PEGASOS), rendered nearly all transparent preserved fluorescence. Bones could turned invisible after clearing. The PEGASOS method adult mouse were able image an head composed bones, teeth, brain, muscles, other no blind areas. transparency enabled us reconstruct intact mandible, femur, knee joint 3-D. managed brain at sub-cellular resolution trace neurons axons over long distance. We also visualized dorsal root ganglions directly through vertebrae. Finally, revealed distribution pattern neural network 3-D marrow space bone. These results suggest is useful tool general biomedical research.

Язык: Английский

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Expansion sequencing: Spatially precise in situ transcriptomics in intact biological systems

Science, Год журнала: 2021, Номер 371(6528)

Опубликована: Янв. 28, 2021

Identifying transcript location in cells where specific RNAs occur within a cell or tissue has been limited by technology and imaging capabilities. Expansion microscopy allowed for better visualization of small structures expanding the tissues with polymer- hydrogel-based system. Alon et al. combined expansion long-read situ RNA sequencing, resulting more precise transcripts. This method, termed “ExSeq” was used to detect RNAs, both new transcripts those previously demonstrated localize neuronal dendrites. Unlike other sequencing methods, ExSeq does not target sets genes. thus unites spatial resolution, multiplexing, an unbiased approach reveal insights into localization its physiological roles developing active tissue. Science , this issue p. eaax2656

Язык: Английский

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