Multi-sensory Gamma Stimulation Ameliorates Alzheimer’s-Associated Pathology and Improves Cognition

Cell, Journal Year: 2019, Volume and Issue: 177(2), P. 256 - 271.e22

Published: March 14, 2019

Language: Английский

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Tissue clearing and its applications in neuroscience

Nature reviews. Neuroscience, Journal Year: 2020, Volume and Issue: 21(2), P. 61 - 79

Published: Jan. 2, 2020

Language: Английский

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Self-organization and symmetry breaking in intestinal organoid development

Nature, Journal Year: 2019, Volume and Issue: 569(7754), P. 66 - 72

Published: April 24, 2019

Language: Английский

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Multiplexed and scalable super-resolution imaging of three-dimensional protein localization in size-adjustable tissues

Nature Biotechnology, Journal Year: 2016, Volume and Issue: 34(9), P. 973 - 981

Published: July 25, 2016

Language: Английский

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Panoptic imaging of transparent mice reveals whole-body neuronal projections and skull–meninges connections

Nature Neuroscience, Journal Year: 2018, Volume and Issue: 22(2), P. 317 - 327

Published: Dec. 20, 2018

Language: Английский

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Protection of tissue physicochemical properties using polyfunctional crosslinkers

Nature Biotechnology, Journal Year: 2018, Volume and Issue: 37(1), P. 73 - 83

Published: Dec. 17, 2018

Language: Английский

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Bioinspired neuron-like electronics

Nature Materials, Journal Year: 2019, Volume and Issue: 18(5), P. 510 - 517

Published: Feb. 25, 2019

Language: Английский

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Self‐organized developmental patterning and differentiation in cerebral organoids

The EMBO Journal, Journal Year: 2017, Volume and Issue: 36(10), P. 1316 - 1329

Published: March 10, 2017

Language: Английский

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Tissue clearing of both hard and soft tissue organs with the PEGASOS method

Cell Research, Journal Year: 2018, Volume and Issue: 28(8), P. 803 - 818

Published: May 29, 2018

Tissue clearing technique enables visualization of opaque organs and tissues in 3-dimensions (3-D) by turning tissue transparent. Current methods are restricted limited types that can be cleared with each individual protocol, which inevitably led to the presence blind-spots within whole body or parts imaging. Hard including bones teeth still most difficult cleared. In addition, loss endogenous fluorescence remains a major concern for solvent-based methods. Here, we developed polyethylene glycol (PEG)-associated solvent system (PEGASOS), rendered nearly all transparent preserved fluorescence. Bones could turned invisible after clearing. The PEGASOS method adult mouse were able image an head composed bones, teeth, brain, muscles, other no blind areas. transparency enabled us reconstruct intact mandible, femur, knee joint 3-D. managed brain at sub-cellular resolution trace neurons axons over long distance. We also visualized dorsal root ganglions directly through vertebrae. Finally, revealed distribution pattern neural network 3-D marrow space bone. These results suggest is useful tool general biomedical research.

Language: Английский

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Expansion sequencing: Spatially precise in situ transcriptomics in intact biological systems

Science, Journal Year: 2021, Volume and Issue: 371(6528)

Published: Jan. 28, 2021

Identifying transcript location in cells where specific RNAs occur within a cell or tissue has been limited by technology and imaging capabilities. Expansion microscopy allowed for better visualization of small structures expanding the tissues with polymer- hydrogel-based system. Alon et al. combined expansion long-read situ RNA sequencing, resulting more precise transcripts. This method, termed “ExSeq” was used to detect RNAs, both new transcripts those previously demonstrated localize neuronal dendrites. Unlike other sequencing methods, ExSeq does not target sets genes. thus unites spatial resolution, multiplexing, an unbiased approach reveal insights into localization its physiological roles developing active tissue. Science , this issue p. eaax2656

Language: Английский

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