A positively tuned voltage indicator for extended electrical recordings in the brain

Nature Methods, Год журнала: 2023, Номер 20(7), С. 1104 - 1113

Опубликована: Июль 1, 2023

Язык: Английский

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Unraveling the dynamics of dopamine release and its actions on target cells

Trends in Neurosciences, Год журнала: 2023, Номер 46(3), С. 228 - 239

Опубликована: Янв. 10, 2023

Язык: Английский

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Visual processing in the fly, from photoreceptors to behavior

Genetics, Год журнала: 2023, Номер 224(2)

Опубликована: Май 2, 2023

Abstract Originally a genetic model organism, the experimental use of Drosophila melanogaster has grown to include quantitative behavioral analyses, sophisticated perturbations neuronal function, and detailed sensory physiology. A highlight these developments can be seen in context vision, where pioneering studies have uncovered fundamental generalizable principles processing. Here we begin with an overview vision-guided behaviors common methods for probing visual circuits. We then outline anatomy physiology brain regions involved processing, beginning at periphery ending descending motor control. Areas focus contrast motion detection optic lobe, circuits feature selectivity, computations support spatial navigation, contextual associative learning. Finally, look future fly neuroscience discuss promising topics further study.

Язык: Английский

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Spatial redundancy transformer for self-supervised fluorescence image denoising

Nature Computational Science, Год журнала: 2023, Номер 3(12), С. 1067 - 1080

Опубликована: Дек. 11, 2023

Abstract Fluorescence imaging with high signal-to-noise ratios has become the foundation of accurate visualization and analysis biological phenomena. However, inevitable noise poses a formidable challenge to sensitivity. Here we provide spatial redundancy denoising transformer (SRDTrans) remove from fluorescence images in self-supervised manner. First, sampling strategy based on is proposed extract adjacent orthogonal training pairs, which eliminates dependence speed. Second, designed lightweight spatiotemporal architecture capture long-range dependencies high-resolution features at low computational cost. SRDTrans can restore high-frequency information without producing oversmoothed structures distorted traces. Finally, demonstrate state-of-the-art performance single-molecule localization microscopy two-photon volumetric calcium imaging. does not contain any assumptions about process sample, thus be easily extended various modalities applications.

Язык: Английский

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Next-Generation Genetically Encoded Fluorescent Biosensors Illuminate Cell Signaling and Metabolism

Annual Review of Biophysics, Год журнала: 2024, Номер 53(1), С. 275 - 297

Опубликована: Фев. 12, 2024

Genetically encoded fluorescent biosensors have revolutionized the study of cell signaling and metabolism, as they allow for live-cell measurements with high spatiotemporal resolution. This success has spurred development tailor-made that enable dynamic phenomena on different timescales length scales. In this review, we discuss approaches to enhancing developing new biosensors. We summarize technologies used gain structural insights into biosensor design comment useful screening technologies. Furthermore, give an overview applications where led key advances over recent years. Finally, our perspective future work is bound make a large impact.

Язык: Английский

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Locus coeruleus norepinephrine contributes to visual-spatial attention by selectively enhancing perceptual sensitivity

Neuron, Год журнала: 2024, Номер 112(13), С. 2231 - 2240.e5

Опубликована: Май 2, 2024

Язык: Английский

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Recent Advances in Transparent Electrodes and Their Multimodal Sensing Applications

Advanced Science, Год журнала: 2024, Номер 11(38)

Опубликована: Авг. 9, 2024

This review examines the recent advancements in transparent electrodes and their crucial role multimodal sensing technologies. Transparent electrodes, notable for optical transparency electrical conductivity, are revolutionizing sensors by enabling simultaneous detection of diverse physical, chemical, biological signals. Materials like graphene, carbon nanotubes, conductive polymers, which offer a balance between transparency, mechanical flexibility, at forefront this development. These integral various applications, from healthcare to solar cell technologies, enhancing sensor performance complex environments. The paper addresses challenges applying these such as need high optoelectronic performance, biocompatibility. It explores new materials innovative techniques overcome hurdles, aiming broaden capabilities devices. provides comparative analysis different electrode materials, discussing applications ongoing development novel systems sensing. exploration offers insights into future highlighting transformative potential bioelectronics

Язык: Английский

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Beyond a Transmission Cable—New Technologies to Reveal the Richness in Axonal Electrophysiology

Journal of Neuroscience, Год журнала: 2024, Номер 44(11), С. e1446232023 - e1446232023

Опубликована: Март 13, 2024

The axon is a neuronal structure capable of processing, encoding, and transmitting information. This assessment contrasts with limiting, but deeply rooted, perspective where the functions solely as transmission cable somatodendritic activity, sending signals in form stereotypical action potentials. arose, at least partially, because technical difficulties probing axons: their extreme length-to-diameter ratio intricate growth paths preclude study dynamics through traditional techniques. Recent findings are challenging this view revealing much larger repertoire axonal computations. Axons display complex signaling processes structure–function relationships, which can be modulated via diverse activity-dependent mechanisms. Additionally, axons exhibit patterns activity that dramatically different from those corresponding soma. Not surprisingly, many these recent discoveries have been driven by novel technology developments, allow for vitro electrophysiology unprecedented spatiotemporal resolution signal-to-noise ratio. In review, we outline state-of-the-art toolset summarize function it has enabled. We also review increasing microtechnologies controlling guidance which, combination available cutting-edge imaging approaches, potential more controlled high-throughput studies. anticipate adoption new technologies neuroscience community will drive era experimental opportunities physiology consequently, function.

Язык: Английский

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Photophysics-informed two-photon voltage imaging using FRET-opsin voltage indicators

Science Advances, Год журнала: 2025, Номер 11(2)

Опубликована: Янв. 8, 2025

Microbial rhodopsin–derived genetically encoded voltage indicators (GEVIs) are powerful tools for mapping bioelectrical dynamics in cell culture and live animals. Förster resonance energy transfer (FRET)–opsin GEVIs use voltage-dependent quenching of an attached fluorophore, achieving high brightness, speed, sensitivity. However, the sensitivity most FRET-opsin has been reported to decrease or vanish under two-photon (2P) excitation. Here, we investigated photophysics Voltron1 Voltron2. We found that previously negative-going sensitivities both came from photocycle intermediates, not opsin ground states. The were nonlinear functions illumination intensity; Voltron1, reversed sign low-intensity illumination. Using photocycle-optimized 2P protocols, demonstrate imaging with Voltron2 barrel cortex a mouse. These results open door high-speed vivo.

Язык: Английский

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Propagation of activity through the cortical hierarchy and perception are determined by neural variability

Nature Neuroscience, Год журнала: 2023, Номер 26(9), С. 1584 - 1594

Опубликована: Авг. 28, 2023

Abstract Brains are composed of anatomically and functionally distinct regions performing specialized tasks, but do not operate in isolation. Orchestration complex behaviors requires communication between brain regions, how neural dynamics organized to facilitate reliable transmission is well understood. Here we studied this process directly by generating activity that propagates drives behavior, assessing populations sensory cortex cooperate transmit information. We achieved imaging two densely interconnected regions—the primary secondary somatosensory (S1 S2)—in mice while two-photon photostimulation S1 neurons assigning behavioral salience the photostimulation. found probability perception determined only strength also variability activity. Therefore, maximizing signal-to-noise ratio stimulus representation relative noise or critical propagation perception.

Язык: Английский

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