Biosensors,
Год журнала:
2025,
Номер
15(3), С. 162 - 162
Опубликована: Март 3, 2025
The
accurate
detection
of
Mycobacterium
tuberculosis
(MTB)
is
a
pressing
challenge
in
the
precise
prevention
and
control
tuberculosis.
Currently,
efficiency
accuracy
drug
resistance
for
MTB
are
low,
cross-contamination
common,
making
it
inadequate
clinical
needs.
This
study
developed
rapid
nucleic
acid
method
based
on
scattering
loop-mediated
isothermal
amplification
(LAMP).
Specific
primers
MTB-specific
gene
(Ag85B)
were
designed,
LAMP
reaction
system
was
optimized
using
self-developed
turbidimeter.
Experimental
results
showed
that
optimal
included
1.5
µL
100
mmol/L
magnesium
ions,
3.5
10
dNTPs,
6
1.6
mol/L
betaine,
temperature
65
°C.
minimum
limit
12.40
ng/L,
with
fastest
time
being
approximately
min.
exhibited
good
specificity,
no
bands
other
pathogens.
Twenty
culture-positive
samples
twenty
culture-negative
tested
parallel;
positive
group
100%,
(24.9
±
13
min),
there
negative
detection.
features
high
efficiency,
low
cost,
accuracy,
effectively
reduces
cross-contamination,
providing
new
technology
MTB.
The Journal of Experimental Medicine,
Год журнала:
2025,
Номер
222(4)
Опубликована: Янв. 17, 2025
Tuberculosis
(TB)
is
a
major
health
burden
worldwide
despite
widespread
intradermal
(ID)
BCG
vaccination
in
newborns.
We
previously
demonstrated
that
changing
the
route
and
dose
from
5
×
105
CFUs
ID
to
107
i.v.
resulted
prevention
of
Mycobacterium
tuberculosis
(Mtb)
infection
TB
disease
highly
susceptible
nonhuman
primates.
Identifying
immune
mechanisms
protection
following
will
facilitate
development
more
effective
vaccines
against
TB.
Here,
we
depleted
lymphocyte
subsets
prior
during
Mtb
challenge
BCG-vaccinated
macaques
identify
those
necessary
for
protection.
Depletion
adaptive
CD4
T
cells,
but
not
CD8αβ
loss
with
increased
burdens
dissemination,
indicating
cells
are
critical
BCG-mediated
unconventional
CD8α-expressing
lymphocytes
(NK
innate
CD4+CD8α+
double-positive
cells)
abrogated
most
BCG-immunized
macaques,
supporting
further
investigation
into
which
these
cell
contribute
after
vaccination.
Frontiers in Immunology,
Год журнала:
2023,
Номер
14
Опубликована: Авг. 17, 2023
Tuberculosis
(TB),
caused
by
Mycobacterium
tuberculosis
(Mtb)
and
Coronavirus
disease-2019
(COVID-19),
whose
etiologic
agent
is
severe
acute
respiratory
syndrome
coronavirus-2
(SARS-CoV-2),
are
currently
the
two
deadliest
infectious
diseases
in
humans,
which
together
have
about
more
than
11
million
deaths
worldwide
past
3
years.
TB
COVID-19
share
several
aspects
including
droplet-
aerosol-borne
transmissibility,
lungs
as
primary
target,
some
symptoms,
diagnostic
tools.
However,
these
differ
other
their
incubation
period,
immune
cells
involved,
persistence
immunopathological
response.
In
this
review,
we
highlight
similarities
differences
between
focusing
on
innate
adaptive
response
induced
after
exposure
to
Mtb
SARS-CoV-2
pathological
pathways
linking
infections.
Moreover,
provide
a
brief
overview
of
case
TB-COVID-19
co-infection
highlighting
each
individual
infection.
A
comprehensive
understanding
involved
utmost
importance
for
design
effective
therapeutic
strategies
vaccines
both
diseases.
Biomedicine & Pharmacotherapy,
Год журнала:
2024,
Номер
171, С. 116087 - 116087
Опубликована: Янв. 2, 2024
Given
that
the
disease
progression
of
tuberculosis
(TB)
is
primarily
related
to
host's
immune
status,
it
has
been
gradually
realized
chemotherapy
targets
bacteria
may
never,
on
its
own,
wholly
eradicate
Mycobacterium
tuberculosis,
causative
agent
TB.
The
concept
host-directed
therapy
(HDT)
with
adjuvants
emerged.
HDT
could
potentially
interfere
infection
and
colonization
by
pathogens,
enhance
protective
responses
hosts,
suppress
overwhelming
inflammatory
responses,
help
attain
a
state
homeostasis
favors
treatment
efficacy.
However,
drugs
currently
being
assessed
in
combination
anti-TB
still
face
dilemmas
arising
from
side
effects
high
costs.
Natural
products
are
well
suited
compensate
for
these
shortcomings
having
gentle
modulatory
host
less
immunopathological
damage
at
lower
cost.
In
this
review,
we
first
summarize
profiles
immunology
characteristics
HDT.
Then,
focus
rationale
challenges
developing
implementing
natural
products-based
A
succinct
report
medications
evaluated
clinical
trials
preclinical
studies
provided.
This
review
aims
promote
target-based
screening
accelerate
novel
TB
drug
discovery.
Heliyon,
Год журнала:
2024,
Номер
10(7), С. e28260 - e28260
Опубликована: Март 21, 2024
Topological
indices
are
molecular
descriptors
used
in
QSPR
modelling
to
predict
the
physicochemical
properties
of
molecules.
numerous
applications
drug
design.
In
this
work,
we
compute
neighbourhood
degree-based
topological
15
antituberculosis
drugs,
studied
analysis
these
drugs
using
support
vector
regression.
The
efficiency
regression
is
determined
by
comparing
it
with
classical
linear
Our
model
further
shows
superiority
SVR
as
a
better
predictive
physical
drugs.
findings
study
contribution
field
chemical
graph
theory
and
design,
providing
deeper
understanding
their
capabilities
model.
Nature Microbiology,
Год журнала:
2023,
Номер
9(1), С. 120 - 135
Опубликована: Дек. 8, 2023
Abstract
Oxidative
stress
triggers
ferroptosis,
a
form
of
cellular
necrosis
characterized
by
iron-dependent
lipid
peroxidation,
and
has
been
implicated
in
Mycobacterium
tuberculosis
(Mtb)
pathogenesis.
We
investigated
whether
Bach1,
transcription
factor
that
represses
multiple
antioxidant
genes,
regulates
host
resistance
to
Mtb.
found
BACH1
expression
is
associated
clinically
with
active
pulmonary
tuberculosis.
Bach1
deletion
Mtb-infected
mice
increased
glutathione
levels
Gpx4
inhibit
peroxidation.
−/−
macrophages
exhibited
Mtb-induced
cell
death,
while
Bach1-deficient
displayed
reduced
bacterial
loads,
peroxidation
concurrent
survival.
Single-cell
RNA-seq
analysis
lungs
from
revealed
an
enrichment
genes
ferroptosis
suppression.
depletion
B6.Sst1
S
display
human-like
necrotic
lung
pathology
also
markedly
resistance.
These
findings
identify
as
key
regulator
tissue
Mtb
infection.
