Antarctic
krill
is
a
species
with
fundamental
importance
for
the
Southern
Ocean
ecosystem.
Their
large
biomass
and
synchronized
movements,
like
diel
vertical
migration
(DVM),
significantly
impact
ecosystem
structure
biological
carbon
pump.
Despite
decades
of
research,
mechanistic
basis
DVM
remains
unclear.
Circadian
clocks
help
organisms
anticipate
daily
environmental
changes,
optimizing
adaptation.
In
this
study,
we
used
recently
developed
activity
monitor
to
record
swimming
individual,
wild-caught
under
various
light
conditions
across
different
seasons.
Our
data
demonstrate
how
circadian
clock,
in
combination
light,
drives
distinct
bimodal
pattern
activity,
which
could
facilitate
ecologically
important
behavioral
patterns,
such
as
DVM.
Rapid
damping
flexible
synchronization
indicate
that
clock
adapted
life
at
high
latitudes
seasonal
recordings
suggest
clock-based
mechanism
timing
processes.
findings
advance
our
understanding
high-latitude
adaptation
key
species.
Long-read
sequencing
data,
particularly
those
derived
from
the
Oxford
Nanopore
platform,
tend
to
exhibit
high
error
rates.
Here,
we
present
NextDenovo,
an
efficient
correction
and
assembly
tool
for
noisy
long
reads,
which
achieves
a
level
of
accuracy
in
genome
assembly.
We
apply
NextDenovo
assemble
35
diverse
human
genomes
around
world
using
long-read
data.
These
allow
us
identify
landscape
segmental
duplication
gene
copy
number
variation
modern
populations.
The
use
should
pave
way
population-scale
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Март 12, 2023
Abstract
Long
read
sequencing
data,
particularly
those
derived
from
the
Oxford
Nanopore
(ONT)
platform,
tend
to
exhibit
a
high
error
rate.
Here,
we
present
NextDenovo,
highly
efficient
correction
and
assembly
tool
for
noisy
long
reads,
which
achieves
level
of
accuracy
in
genome
assembly.
NextDenovo
can
rapidly
correct
reads;
these
corrected
reads
contain
fewer
errors
than
other
comparable
tools
are
characterized
by
chimeric
alignments.
We
applied
quality
reference
genomes
35
diverse
humans
across
world
using
ONT
data.
Based
on
de
novo
assemblies,
were
able
identify
landscape
segmental
duplications
gene
copy
number
variation
modern
human
population.
The
use
program
should
pave
way
population-scale
long-read
assembly,
thereby
facilitating
construction
pan-genomes,
Abstract
Background
Due
to
its
enormous
biomass,
Antarctic
krill
(
Euphausia
superba
)
plays
a
crucial
role
in
the
Ocean
ecosystem.
In
recent
years,
has
found
extensive
application
aquaculture,
emerging
as
sustainable
source
of
aquafeed
with
ideal
nutritional
profiles.
However,
comprehensive
study
focused
on
detailed
effects
dietary
aquaculture
animals,
especially
farmed
marine
fishes,
is
yet
be
demonstrated.
Results
this
study,
comparative
experiment
was
performed
using
juvenile
P.
leopardus
,
fed
diets
supplemented
(the
group)
or
without
control
group).
Histological
observation
revealed
that
could
reduce
lipid
accumulation
liver
while
intestine
exhibited
no
obvious
changes.
Enzyme
activity
measurements
demonstrated
had
an
inhibitory
effect
oxidative
stress
both
and
liver.
By
transcriptome
analysis,
total
1,597
1,161
differentially
expressed
genes
(DEGs)
were
identified
liver,
respectively.
Functional
analysis
DEGs
showed
multiple
enriched
terms
significantly
related
cholesterol
metabolism,
antioxidants,
immunity.
Furthermore,
expression
profiles
representative
DEGs,
such
dhcr7
apoa4
sc5d
scarf1
validated
by
qRT-PCR
fluorescence
situ
hybridization.
Finally,
demonstrate
biased
astaxanthin
.
Conclusions
Our
enhance
antioxidant
capacities
exhibit
molecular-level
improvements
immunity,
antioxidants.
It
will
contribute
understanding
protective
provide
insights
into
strategies.
Abstract
Long
noncoding
RNAs
(lncRNAs),
which
are
more
than
200
nucleotides
in
length
and
do
not
encode
proteins,
play
crucial
roles
governing
gene
expression
at
both
the
transcriptional
posttranscriptional
levels.
These
molecules
demonstrate
specific
patterns
various
tissues
developmental
stages,
suggesting
their
involvement
numerous
processes
diseases,
notably
cancer.
Despite
widespread
acknowledgment
growing
enthusiasm
surrounding
potential
as
diagnostic
prognostic
biomarkers,
precise
mechanisms
through
lncRNAs
function
remain
inadequately
understood.
A
few
have
been
studied
depth,
providing
valuable
insights
into
biological
activities
emerging
functional
themes
mechanistic
models.
However,
extent
to
mammalian
genome
is
transcribed
transcripts
still
a
matter
of
debate.
This
review
synthesizes
our
current
understanding
lncRNA
biogenesis,
genomic
contexts,
multifaceted
tumorigenesis,
highlighting
cancer-targeted
therapy.
By
exploring
historical
perspectives
alongside
recent
breakthroughs,
we
aim
illuminate
diverse
reflect
on
broader
implications
study
for
evolution
function,
well
advancing
clinical
applications.
