Communications Biology,
Год журнала:
2025,
Номер
8(1)
Опубликована: Фев. 24, 2025
The
NADase
activity
of
the
defense-associated
sirtuins
(DSRs)
is
activated
by
phage
tail
tube
protein
(TTP).
Herein,
we
report
cryo-EM
structures
a
free-state
Bacillus
subtilis
DSR2
tetramer
and
fragment
tetramer,
SPR
tube,
two
DSR2-TTP
complexes.
contains
an
N-terminal
SIR2
domain,
middle
domain
(MID)
C-terminal
(CTD).
CTD
harbors
α-solenoid
tandem-repeats
like
HEAT-repeat
proteins.
assembles
into
with
four
clustered
at
center,
intertwined
MID-CTD
chains
flank
core.
TTPs
self-assemble
tube-like
complex.
Upon
binding,
D1
TTP
captured
between
HEAT-repeats
domains
DSR2,
which
conflicts
self-assembly.
Binding
induces
conformational
changes
in
resulting
increase
NAD+
pocket
volume
SIR2,
thus
activates
leads
to
cellular
depletion.
Cryo-EM
reveal
that
sirtuin,
recognizes
protein,
activating
its
elucidating
role
bacterial
anti-phage
immunity
pathogen
recognition.
Prokaryotes
encode
multiple
distinct
anti-phage
defense
systems
in
their
genomes.
However,
the
impact
of
carrying
a
multitude
on
phage
resistance
remains
unclear,
especially
clinical
context.
Using
collection
antibiotic-resistant
strains
Pseudomonas
aeruginosa
and
broad
panel
phages,
we
demonstrate
that
contribute
substantially
to
defining
host
range
overall
scales
with
number
bacterial
genome.
We
show
many
individual
target
specific
genera
complementary
specificities
co-occur
P.
genomes
likely
provide
benefits
phage-diverse
environments.
Overall,
phage-resistant
phenotypes
at
least
19
exist
populations
clinical,
strains.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 26, 2024
In
recent
years,
a
vast
number
of
novel
antiphage
defense
mechanisms
were
uncovered.
To
facilitate
the
exploration
mechanistic,
ecological,
and
evolutionary
aspects
related
to
systems,
we
released
DefenseFinder
in
2021
(Tesson
et
al.,
2022).
is
bioinformatic
program
designed
for
systematic
identification
known
mechanisms.
The
initial
release
v1.0.0
included
60
systems.
Over
past
three
systems
incorporated
into
has
grown
152.
increasing
makes
it
challenge
enter
field
interpretation
detections
difficult.
Moreover,
rapid
development
sequence-based
predictions
structures
offers
possibilities
analysis
should
be
easily
available.
overcome
these
challenges,
present
hub
resources
on
including:
1)
an
updated
version
with
web-service
search
function,
2)
community-curated
repository
knowledge
3)
precomputed
databases,
which
include
annotations
done
RefSeq
genomes
structure
generated
by
AlphaFold.
These
pages
can
freely
accessed
users
as
starting
point
their
journey
better
understand
given
system.
We
anticipate
that
will
foster
use
bioinformatics
study
serve
community
researchers
who
This
resource
available
at:
https://defensefinder.mdmlab.fr.
Current Opinion in Microbiology,
Год журнала:
2024,
Номер
78, С. 102436 - 102436
Опубликована: Фев. 17, 2024
Bacteria
have
evolved
a
variety
of
defence
mechanisms
to
protect
against
mobile
genetic
elements,
including
restriction-modification
systems
and
CRISPR–Cas.
In
recent
years,
dozens
previously
unknown
(DSs)
been
discovered.
Notably,
diverse
DSs
often
coexist
within
the
same
genome,
some
co-occur
at
frequencies
significantly
higher
than
would
be
expected
by
chance,
implying
potential
synergistic
interactions.
Recent
studies
provided
evidence
that
enhance
or
complement
one
another.
Here,
we
review
interactions
between
mechanistic,
regulatory,
ecological
evolutionary
levels.
PLoS Biology,
Год журнала:
2024,
Номер
22(7), С. e3002717 - e3002717
Опубликована: Июль 15, 2024
Immune
defence
mechanisms
exist
across
the
tree
of
life
in
such
diversity
that
prokaryotic
antiviral
responses
have
historically
been
considered
unrelated
to
eukaryotic
immunity.
Mechanisms
divergent
eukaryotes
were
similarly
believed
be
largely
clade
specific.
However,
recent
data
indicate
a
subset
modules
(domains
and
proteins)
from
prokaryote
systems
are
conserved
populate
many
stages
innate
immune
pathways.
In
this
Essay,
we
propose
notion
ancestral
immunity,
which
corresponds
set
between
prokaryotes
eukaryotes.
After
offering
typology
speculate
on
selective
pressures
could
led
differential
conservation
specific
domains
life.
The
exploration
immunity
is
its
infancy
appears
full
promises
illuminate
evolution,
also
identify
decipher
economic,
ecological,
therapeutic
importance.
