Nature Ecology & Evolution, Год журнала: 2024, Номер 8(4), С. 593 - 594
Опубликована: Апрель 11, 2024
Язык: Английский
Science, Год журнала: 2024, Номер 384(6697)
Опубликована: Апрель 25, 2024
Gasdermins (GSDMs) are pore-forming proteins that execute pyroptosis for immune defense. GSDMs two-domain activated by proteolytic removal of the inhibitory domain. In this work, we report two types cleavage-independent GSDM activation. First, Tricho GSDM, a domain–only protein from basal metazoan Trichoplax adhaerens , is disulfides-linked autoinhibited dimer reduction disulfides. The cryo–electron microscopy (cryo-EM) structure illustrates assembly mechanism 44-mer pore. Second, RCD-1-1 and RCD-1-2, encoded polymorphic regulator cell death–1 ( rcd-1 ) gene in filamentous fungus Neurospora crassa also GSDMs. when encountering each other, form pores cause pyroptosis, underlying allorecognition . cryo-EM reveals pore 11 RCD-1-1/RCD-1-2 heterodimers heterodimerization-triggered mechanism. This study shows mechanistic diversities activation indicates versatile functions
Язык: Английский
Процитировано
18
Current Biology, Год журнала: 2024, Номер 34(2), С. 361 - 375.e9
Опубликована: Янв. 1, 2024
A hallmark of animals is the coordination whole-body movement. Neurons and muscles are central to this, yet coordinated movements also exist in sponges that lack these cell types. Sponges sessile with a complex canal system for filter-feeding. They undergo resembling "contractions" lead closure water expulsion. Here, we combine live 3D optical coherence microscopy, pharmacology, functional proteomics elucidate sequence detail shape changes, tissues molecular physiology involved, control movements. Morphometric analysis targeted perturbation suggest movement driven by relaxation actomyosin stress fibers epithelial cells, which leads deflation via collapse incurrent expansion excurrent system. Thermal proteome profiling quantitative phosphoproteomics confirm cellular an Akt/NO/PKG/PKA pathway. Agitation-induced differential phosphorylation proteins forming junctions, implying their mechanosensitive role. Unexpectedly, untargeted metabolomics detect concomitant decrease antioxidant molecules during deflation, reflecting increase reactive oxygen species. Together secretion proteinases, cytokines, granulin, this indicates inflammation-like state deflating sponge reminiscent vascular endothelial cells experiencing oscillatory shear stress. These results conservation ancient relaxant-inflammatory response perturbed fluid-carrying systems offer possible mechanism through diffusible paracrine signals mechanotransduction.
Язык: Английский
Процитировано
11
Cell stem cell, Год журнала: 2024, Номер 31(7), С. 1038 - 1057.e11
Опубликована: Май 10, 2024
Язык: Английский
Процитировано
7
Annual Review of Cell and Developmental Biology, Год журнала: 2024, Номер 40(1), С. 51 - 74
Опубликована: Июль 4, 2024
Instances of multicellularity across the tree life have fostered evolution complex organs composed distinct cell types that cooperate, producing emergent biological functions. How originate is a fundamental evolutionary problem has eluded deep mechanistic and conceptual understanding. Here I propose cell- to organ-level transitions framework, whereby cooperative division labor originates becomes entrenched between through process functional niche creation, cell-type subfunctionalization, irreversible ratcheting interdependencies. Comprehending this transition hinges on explaining how these processes unfold molecularly in evolving populations. Recent single-cell transcriptomic studies analyses terminal fate specification indicate cellular functions are conferred by modular gene expression programs. These discrete components variation may be deployed or combined within cells introduce new properties into multicellular niches, partitioned establish labor. Tracing program at level single populations reveal toward organ complexity.
Язык: Английский
Процитировано
7
Annual Review of Cell and Developmental Biology, Год журнала: 2024, Номер 40(1), С. 353 - 379
Опубликована: Июль 10, 2024
Sensory receptors are at the interface between an organism and its environment thus represent key sites for biological innovation. Here, we survey major sensory receptor families to uncover emerging evolutionary patterns. Receptors touch, temperature, light constitute part of ancestral toolkit animals, often predating evolution multicellularity nervous system. In contrast, chemoreceptors exhibit a dynamic history lineage-specific expansions contractions correlated with disparate complexity chemical environments. A recurring theme includes independent transitions from neurotransmitter diverse stimuli outside world. We then provide overview mechanisms underlying diversification highlight examples where signatures natural selection used identify novel adaptations. Finally, discuss as hotspots driving reproductive isolation speciation, thereby contributing stunning diversity animals.
Язык: Английский
Процитировано
7
The Journal of Open Source Software, Год журнала: 2024, Номер 9(100), С. 7012 - 7012
Опубликована: Авг. 19, 2024
Sequence motifs are an important concept in molecular biology, as specific repeating patterns DNA, RNA and proteins form the basis of biological regulation.Identifying characterizing these is therefore a part studying various aspects cellular processes, such gene regulation, transcript stability, protein function.Many programs have been developed over years to tackle tasks, though their interoperability remains poor.The universalmotif package has two main goals: serve go-between for most common motif Bioconductor packages used by research community, provide robust set tools basic analysis manipulation R. Tools sequence manipulation, scanning, enrichment, comparison, shuffling P-value computation included. InstallationThe project including its extensive documentation hosted on Bioconductor, with pre-built binaries available macOS Windows (and installation from source all platforms).Installation takes place within R using BiocManager package, which itself can be installed CRAN:install.packages("BiocManager") BiocManager::install("universalmotif")
Язык: Английский
Процитировано
7
Science Advances, Год журнала: 2025, Номер 11(2)
Опубликована: Янв. 8, 2025
The transition from simple to complex multicellularity involves division of labor and specialization cell types. In animals, sensory-motor systems are primarily built around specialized cells muscles neurons, though the evolutionary origins these their integration remain unclear. Here, investigate sensory-behavior coupling in closest relatives we established a line choanoflagellate, Salpingoeca rosetta, which stably expresses calcium indicator RGECO1. Using this, identify previously unknown cellular behavior associated with electrical signaling, ciliary arrest is coupled apical-basal contraction cell. This transients synchronized multicellular state result coordinated colony-wide contraction, suggesting that information spread among cells. Our work reveals fundamental insights into how choanoflagellates sense respond environment enhances our understanding organism-wide protistan animals.
Язык: Английский
Процитировано
1
Zoologica Scripta, Год журнала: 2024, Номер 53(6), С. 754 - 762
Опубликована: Июнь 21, 2024
Abstract Molecular markers for tracing animal sample origins and compositions are critical applications such as parasite detection, contamination screening, authentication. Among these, microRNAs have emerged promising candidates due to their deep conservation, near‐hierarchical evolution, stability. I here review the suitability of taxonomic also phylogenetic show how careful annotation efforts establishment curated microRNA gene database MirGeneDB tools like MirMachine revitalized research. These advancements enable accurate studies, highlighting microRNAs' potential in resolving long‐standing questions relationships extending ancient DNA environmental RNA analysis. Future research must focus on expanding complements across all Metazoa further improving methodologies.
Язык: Английский
Процитировано
6
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Янв. 9, 2024
Enteroendocrine cells (EECs), which secrete serotonin (enterochromaffin cells, EC) or a dominant peptide hormone, serve vital physiologic functions. As with any adult human lineage, the basis for terminal cell diversity remains obscure. We replicated EEC differentiation
Язык: Английский
Процитировано
5
Nature Communications, Год журнала: 2024, Номер 15(1)
Опубликована: Ноя. 14, 2024
Stem cells are a hallmark of animal multicellularity. Sox and POU transcription factors associated with stemness were believed to be innovations, reported absent in their unicellular relatives. Here we describe factors. Choanoflagellate filasterean proteins have DNA-binding specificity similar mammalian Sox2. Choanoflagellate—but not filasterean—Sox can replace Sox2 reprogram mouse somatic into induced pluripotent stem (iPSCs) through interacting the member Oct4. In contrast, choanoflagellate has distinct profile cannot generate iPSCs. Ancestrally reconstructed indicate that iPSC formation capacity is pervasive among resurrected sequences, thus loss Sox2-like properties fostered family subfunctionalization. Our findings imply evolution might involved exaptation pre-existing set factors, where pre-animal was biochemically extant Sox, whilst required evolutionary innovations. The pluripotency program maintained by from families. they identify SOX relatives animals show SOX2 induce pluripotency, differs OCT4.
Язык: Английский
Процитировано
5