The connectome of the adult Drosophila mushroom body provides insights into function

eLife, Год журнала: 2020, Номер 9

Опубликована: Дек. 14, 2020

Making inferences about the computations performed by neuronal circuits from synapse-level connectivity maps is an emerging opportunity in neuroscience. The mushroom body (MB) well positioned for developing and testing such approach due to its conserved architecture, recently completed dense connectome, extensive prior experimental studies of roles learning, memory, activity regulation. Here, we identify new components MB circuit Drosophila, including visual input output neurons (MBONs) with direct connections descending neurons. We find unexpected structure sensory inputs, transfer information different modalities MBONs, modulation that dopaminergic (DANs). provide insights into circuitry used integrate outputs, between central complex inputs DANs, feedback MBONs. Our results a foundation further theoretical work.

Язык: Английский

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A connectome of the Drosophila central complex reveals network motifs suitable for flexible navigation and context-dependent action selection

eLife, Год журнала: 2021, Номер 10

Опубликована: Окт. 26, 2021

Flexible behaviors over long timescales are thought to engage recurrent neural networks in deep brain regions, which experimentally challenging study. In insects, circuit dynamics a region called the central complex (CX) enable directed locomotion, sleep, and context- experience-dependent spatial navigation. We describe first complete electron microscopy-based connectome of

Язык: Английский

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Information flow, cell types and stereotypy in a full olfactory connectome

eLife, Год журнала: 2021, Номер 10

Опубликована: Май 25, 2021

The

Язык: Английский

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Neuronal wiring diagram of an adult brain

Nature, Год журнала: 2024, Номер 634(8032), С. 124 - 138

Опубликована: Окт. 2, 2024

Язык: Английский

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Neuronal wiring diagram of an adult brain

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Июнь 30, 2023

Abstract Connections between neurons can be mapped by acquiring and analyzing electron microscopic (EM) brain images. In recent years, this approach has been applied to chunks of brains reconstruct local connectivity maps that are highly informative, yet inadequate for understanding function more globally. Here, we present the first neuronal wiring diagram a whole adult brain, containing 5×10 7 chemical synapses ∼130,000 reconstructed from female Drosophila melanogaster . The resource also incorporates annotations cell classes types, nerves, hemilineages, predictions neurotransmitter identities. Data products available download, programmatic access, interactive browsing made interoperable with other fly data resources. We show how derive projectome, map projections regions, connectome. demonstrate tracing synaptic pathways analysis information flow inputs (sensory ascending neurons) outputs (motor, endocrine, descending neurons), across both hemispheres, central optic lobes. Tracing subset photoreceptors all way motor illustrates structure uncover putative circuit mechanisms underlying sensorimotor behaviors. technologies open ecosystem FlyWire Consortium set stage future large-scale connectome projects in species.

Язык: Английский

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Transforming representations of movement from body- to world-centric space

Nature, Год журнала: 2021, Номер 601(7891), С. 98 - 104

Опубликована: Дек. 15, 2021

Язык: Английский

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Whole-brain annotation and multi-connectome cell typing of Drosophila

Nature, Год журнала: 2024, Номер 634(8032), С. 139 - 152

Опубликована: Окт. 2, 2024

Язык: Английский

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Automated synapse-level reconstruction of neural circuits in the larval zebrafish brain

Nature Methods, Год журнала: 2022, Номер 19(11), С. 1357 - 1366

Опубликована: Окт. 24, 2022

Abstract Dense reconstruction of synaptic connectivity requires high-resolution electron microscopy images entire brains and tools to efficiently trace neuronal wires across the volume. To generate such a resource, we sectioned imaged larval zebrafish brain by serial block-face at voxel size 14 × 25 nm 3 . We segmented resulting dataset with flood-filling network algorithm, automated detection chemical synapses validated results comparisons transmission microscopic light-microscopic reconstructions. Neurons their connections are stored in form queryable expandable digital address book. reconstructed 208 neurons involved visual motion processing, most them located pretectum, which had been functionally characterized same specimen two-photon calcium imaging. Moreover, mapped all 407 presynaptic postsynaptic partners two superficial interneurons tectum. The resource developed here serves as foundation for synaptic-resolution circuit analyses nervous system.

Язык: Английский

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Hierarchical architecture of dopaminergic circuits enables second-order conditioning in Drosophila

eLife, Год журнала: 2023, Номер 12

Опубликована: Янв. 24, 2023

Dopaminergic neurons with distinct projection patterns and physiological properties compose memory subsystems in a brain. However, it is poorly understood whether or how they interact during complex learning. Here, we identify feedforward circuit formed between dopamine show that essential for second-order conditioning, an ethologically important form of higher-order associative The Drosophila mushroom body comprises series dopaminergic compartments, each which exhibits dynamics. We find slow stable compartment can serve as effective ‘teacher’ by instructing other faster transient compartments via single key interneuron, connectome analysis neurotransmitter prediction. This excitatory interneuron acquires enhanced response to reward-predicting odor after first-order conditioning and, upon activation, evokes release the ‘student’ compartments. These hierarchical connections explain first- long known behavioral psychologists.

Язык: Английский

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Generating parallel representations of position and identity in the olfactory system

Cell, Год журнала: 2023, Номер 186(12), С. 2556 - 2573.e22

Опубликована: Май 25, 2023

In Drosophila, a dedicated olfactory channel senses male pheromone, cis-vaccenyl acetate (cVA), promoting female courtship while repelling males. Here, we show that separate cVA-processing streams extract qualitative and positional information. cVA sensory neurons respond to concentration differences in 5-mm range around male. Second-order projection encode the angular position of by detecting inter-antennal concentration, which are amplified through contralateral inhibition. At third circuit layer, identify 47 cell types with diverse input-output connectivity. One population responds tonically flies, second is tuned looming, integrates taste coincidentally promote mating. The separation features resembles mammalian what where visual streams; together multisensory integration, this enables behavioral responses appropriate specific ethological contexts.

Язык: Английский

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