Techniques
that
enable
precise
manipulations
of
subsets
neurons
in
the
fly
central
nervous
system
have
greatly
facilitated
our
understanding
neural
basis
behavior.
Split-GAL4
driver
lines
allow
specific
targeting
cell
types
Drosophila
melanogaster
and
other
species.
We
describe
here
a
collection
3060
range
adult
1373
characterized
third-instar
larvae.
These
tools
functional,
transcriptomic,
proteomic
studies
based
on
anatomical
targeting.
NeuronBridge
search
relate
light
microscopy
images
these
split-GAL4
to
connectomes
reconstructed
from
electron
images.
The
collections
are
result
screening
over
77,000
split
hemidriver
combinations.
Previously
published
new
included,
all
validated
for
expression
curated
optimal
type
specificity
across
diverse
types.
In
addition
stocks
well-characterized
lines,
we
make
available
300,000
3D
lines.
Nature,
Год журнала:
2025,
Номер
640(8058), С. 435 - 447
Опубликована: Апрель 9, 2025
Abstract
Understanding
the
brain
requires
understanding
neurons’
functional
responses
to
circuit
architecture
shaping
them.
Here
we
introduce
MICrONS
connectomics
dataset
with
dense
calcium
imaging
of
around
75,000
neurons
in
primary
visual
cortex
(VISp)
and
higher
areas
(VISrl,
VISal
VISlm)
an
awake
mouse
that
is
viewing
natural
synthetic
stimuli.
These
data
are
co-registered
electron
microscopy
reconstruction
containing
more
than
200,000
cells
0.5
billion
synapses.
Proofreading
a
subset
yielded
reconstructions
include
complete
dendritic
trees
as
well
local
inter-areal
axonal
projections
map
up
thousands
cell-to-cell
connections
per
neuron.
Released
open-access
resource,
this
includes
tools
for
retrieval
analysis
1,2
.
Accompanying
studies
describe
its
use
comprehensive
characterization
cell
types
3–6
,
synaptic
level
connectivity
diagram
cortical
column
4
uncovering
cell-type-specific
inhibitory
can
be
linked
gene
expression
4,7
Functionally,
identify
new
computational
principles
how
information
integrated
across
space
8
characterize
novel
neuronal
invariances
9
bring
structure
function
together
uncover
general
principle
between
excitatory
within
10,11
In
both
invertebrates
such
as
Drosophila
and
vertebrates
mouse
or
human,
the
brain
contains
most
diverse
population
of
cell
types
any
tissue.
It
is
generally
accepted
that
transcriptional
diversity
an
early
step
in
generating
neuronal
glial
diversity,
followed
by
establishment
a
unique
gene
expression
profile
determines
morphology,
connectivity,
function.
,
there
are
two
neural
stem
cells,
called
Type
1
(T1)
2
(T2)
neuroblasts.
contrast
to
T1
neuroblasts,
T2
neuroblasts
generate
intermediate
progenitors
(INPs)
expand
number
types.
The
T2-derived
neurons
contributes
large
portion
central
complex
(CX),
conserved
region
plays
role
sensorimotor
integration.
Recent
work
has
revealed
much
connectome
CX,
but
how
this
assembled
remains
unclear.
Mapping
derived
from
necessary
linking
assembly
adult
brain.
Here
we
perform
single
nuclei
RNA
sequencing
neuroblast-derived
glia.
We
identify
clusters
containing
all
known
classes
glia,
male/female
enriched,
161
neuron-specific
clusters.
map
neurotransmitter
neuropeptide
transcription
factor
combinatorial
codes
for
each
cluster
(presumptive
neuron
subtype).
This
directs
functional
studies
determine
whether
code
specifies
distinct
type
within
CX.
several
columnar
subtypes
(NPF+
AstA+)
closely
related
Our
data
support
hypothesis
represents
one
few
subtypes.
Abstract
Vision
provides
animals
with
detailed
information
about
their
surroundings
and
conveys
diverse
features
such
as
colour,
form
movement
across
the
visual
scene.
Computing
these
parallel
spatial
requires
a
large
network
of
neurons.
Consequently,
from
flies
to
humans,
regions
in
brain
constitute
half
its
volume.
These
often
have
marked
structure–function
relationships,
neurons
organized
along
maps
shapes
that
directly
relate
roles
processing.
More
than
century
anatomical
studies
catalogued
detail
cell
types
fly
systems
1–3
,
behavioural
physiological
experiments
examined
capabilities
flies.
To
unravel
diversity
complex
system,
careful
mapping
neural
architecture
matched
tools
for
targeted
exploration
this
circuitry
is
essential.
Here
we
present
connectome
right
optic
lobe
male
Drosophila
melanogaster
acquired
using
focused
ion
beam
milling
scanning
electron
microscopy.
We
established
comprehensive
inventory
developed
computational
framework
quantify
anatomy.
Together,
data
establish
basis
interpreting
how
vision.
By
integrating
analysis
connectivity
information,
neurotransmitter
identity
expert
curation,
classified
approximately
53,000
into
732
types.
are
systematically
described
newly
named.
Finally,
share
an
extensive
collection
split-GAL4
lines
our
neuron-type
catalogue.
Overall,
set
unlocks
new
possibilities
systematic
investigations
vision
foundation
deeper
understanding
sensory
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 10, 2024
Abstract
Techniques
that
enable
precise
manipulations
of
subsets
neurons
in
the
fly
central
nervous
system
have
greatly
facilitated
our
understanding
neural
basis
behavior.
Split-GAL4
driver
lines
allow
specific
targeting
cell
types
Drosophila
melanogaster
and
other
species.
We
describe
here
a
collection
3060
range
adult
1373
characterized
third-instar
larvae.
These
tools
functional,
transcriptomic,
proteomic
studies
based
on
anatomical
targeting.
NeuronBridge
search
relate
light
microscopy
images
these
split-GAL4
to
connectomes
reconstructed
from
electron
images.
The
collections
are
result
screening
over
77,000
split
hemidriver
combinations.
Previously
published
new
included,
all
validated
for
expression
curated
optimal
type
specificity
across
diverse
types.
In
addition
stocks
well-characterized
lines,
we
make
available
300,000
3D
lines.
