The
claustrum
is
thought
to
be
one
of
the
most
highly
interconnected
forebrain
structures
but
its
organizing
principles
have
yet
fully
explored
at
level
single
neurons.
Here,
we
investigated
identity,
connectivity,
and
activity
identified
neurons
understand
how
structure’s
unique
convergence
input
divergence
output
support
binding
information
streams.
We
found
that
in
communicate
with
each
other
across
efferent
projection-defined
modules
which
were
differentially
innervated
by
sensory
frontal
cortical
areas.
Individual
responsive
inputs
from
more
than
region
a
cell-type
projection-specific
manner,
particularly
between
areas
cortex.
In
vivo
imaging
axons
revealed
responses
both
unimodal
multimodal
stimuli.
Finally,
chronic
silencing
specifically
reduced
animals’
sensitivity
These
findings
view
fundamentally
integrative
structure,
consolidating
around
cortex
redistributing
it
following
local
computations.
Cell Reports,
Год журнала:
2024,
Номер
43(2), С. 113748 - 113748
Опубликована: Фев. 1, 2024
Animals
are
known
to
exhibit
innate
and
learned
forms
of
defensive
behaviors,
but
it
is
unclear
whether
animals
can
escape
through
methods
other
than
these
forms.
In
this
study,
we
develop
the
delayed
task,
in
which
male
rats
temporarily
hold
information
required
for
future
escape,
demonstrate
that
subject
extrapolates
from
past
experience
without
direct
its
behavioral
outcome,
does
not
fall
into
either
two
behavior.
During
holding
period,
a
subset
neurons
rostral-to-striatum
claustrum
(rsCla),
only
when
pooled
together,
sustain
enhanced
population
activity
ongoing
sensory
stimuli.
Transient
inhibition
rsCla
during
initial
part
period
produces
prolonged
activity.
The
transient
also
attenuates
Our
data
suggest
bridges
escape-inducing
stimuli
onset
escape.
Neuroinformatics,
Год журнала:
2024,
Номер
22(3), С. 251 - 268
Опубликована: Май 20, 2024
Abstract
Sensorimotor
computation
integrates
bottom-up
world
state
information
with
top-down
knowledge
and
task
goals
to
form
action
plans.
In
the
rodent
whisker
system,
a
prime
model
of
active
sensing,
evidence
shows
neuromodulatory
neurotransmitters
shape
control,
affecting
whisking
frequency
amplitude.
Since
are
mostly
released
from
subcortical
nuclei
have
long-range
projections
that
reach
rest
central
nervous
mapping
circuits
control
sensorimotor
will
help
systematically
address
mechanisms
sensing.
Therefore,
we
developed
neuroinformatic
target
discovery
pipeline
mine
Allen
Institute’s
Mouse
Brain
Connectivity
Atlas.
Using
network
connectivity
analysis,
identified
new
putative
connections
along
system
anatomically
confirmed
existence
42
previously
unknown
monosynaptic
connections.
this
data,
updated
map
mouse
first
cell-type-specific
network.
The
includes
157
across
18
principal
neurotransmitter-releasing.
Performing
graph
analysis
connectome,
cell-type
specific
hubs,
sources,
sinks,
provided
anatomical
for
inhibitory
into
all
stages
ascending
pathway,
showed
improve
network-wide
connectivity.
These
results
argue
beyond
modulatory
chemical
contributions
processing
transfer
in
circuit
features
networks
position
them
as
nodes
sensory
motor
integration.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Окт. 17, 2024
Slow
waves
are
a
distinguishing
feature
of
non-rapid-eye-movement
(NREM)
sleep,
an
evolutionarily
conserved
process
critical
for
brain
function.
Non-human
studies
suggest
that
the
claustrum,
small
subcortical
nucleus,
coordinates
slow
waves.
We
show
that,
in
contrast
to
neurons
from
other
regions,
claustrum
human
increase
their
spiking
activity
and
track
during
NREM
suggesting
plays
role
coordinating
sleep
architecture.
Cerebral Cortex,
Год журнала:
2022,
Номер
33(7), С. 3944 - 3959
Опубликована: Сен. 14, 2022
Abstract
The
claustrum
is
known
for
its
extensive
connectivity
with
many
other
forebrain
regions,
but
elongated
shape
and
deep
location
have
made
further
study
difficult.
We
sought
to
understand
when
mouse
neurons
are
born,
where
they
located
in
developing
brains,
develop
their
widespread
connections
the
cortex.
established
that
a
well-characterized
parvalbumin
plexus,
which
identifies
adults,
only
present
from
postnatal
day
(P)
21.
A
myeloarchitectonic
outline
of
can
be
derived
triangular
fiber
arrangement
P15.
dense
patch
Nurr1+
cells
at
core
already
evident
birth.
Bromodeoxyuridine
birth
dating
progenitors
reveals
majority
born
during
narrow
time
window
centered
on
embryonic
12.5,
later
than
adjacent
subplate
endopiriform
nucleus.
Retrograde
tracing
revealed
projections
anterior
cingulate
(ACA)
retrosplenial
cortex
(RSP)
follow
distinct
developmental
trajectories.
Claustrum–ACA
matures
rapidly
reaches
adult-like
innervation
density
by
P10,
whereas
claustrum–RSP
emerges
over
protracted
window.
This
work
establishes
timeline
development
provides
framework
understanding
how
built
develops
unique
connectivity.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Ноя. 27, 2023
The
mammalian
brain
is
composed
of
diverse
neuron
types
that
play
different
functional
roles.
Recent
single-cell
RNA
sequencing
approaches
have
led
to
a
whole
taxonomy
transcriptomically-defined
cell
types,
yet
type
definitions
include
multiple
cellular
properties
can
offer
additional
insights
into
neuron's
role
in
circuits.
While
the
Patch-seq
method
investigate
how
transcriptomic
relate
local
morphological
and
electrophysiological
linking
identities
long-range
projections
major
unresolved
challenge.
To
address
this,
we
collected
coordinated
morphology
data
sets
excitatory
neurons
mouse
visual
cortex.
From
data,
defined
16
integrated
morpho-electric-transcriptomic
(MET)-types;
parallel,
reconstructed
complete
morphologies
300
neurons.
We
unified
two
with
multi-step
classifier,
integrate
assignments
interrogate
cross-modality
relationships.
find
variations
within
across
MET-types
correspond
phenotypes.
In
addition,
this
variation,
along
anatomical
location
cell,
be
used
predict
projection
targets
individual
also
shed
new
light
on
infragranular
circuits,
including
cell-type-specific,
interhemispheric
projections.
With
approach,
establish
comprehensive,
cortex
create
system
for
integrated,
high-dimensional
classification
extended
potentially
species.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Март 24, 2023
Abstract
The
claustrum
(CLA)
is
a
brain
nucleus
located
between
the
insula
and
lateral
striatum,
implicated
in
wide
range
of
behaviors.
Underpinning
different
behavioral
phenotypes
connectivity
various
cortical
regions,
including
anterior
cingulate
cortex
(ACC).
CLA
projection
neurons
are
glutamatergic
neurons,
however,
impact
on
its
targets
has
been
shown
some
studies
to
be
inhibitory.
Such
inhibition
likely
arise
from
claustral
activation
interneurons,
intricate
synaptic
cell
types
not
known.
Here,
we
combine
vivo
ex
electrophysiology
optogenetics
reveal
functional
organization
CLA-ACC
pathway
according
identity
pre-
postsynaptic
populations.
Optogenetic
stimulation
awake
mice
resulted
multiphasic
excitatory
inhibitory
responses
ACC
cells,
which
depended
layer,
type,
stimulated
population.
Using
paired
recordings
ACC,
monosynaptic
were
recorded
pyramidal
cells
interneurons
following
photostimulation
terminals.
axons
formed
connections
all
layers,
but
probability
strength
type
projection,
target
layer
neuron.
This
may
explain
complex
other
thus
resolving
discrepancies
field
shedding
light
role
plays
function.
A
substantial
proportion
of
raphe
neurons
are
glutamatergic.
However,
little
is
known
about
how
these
glutamatergic
modulate
the
forebrain.
We
investigated
median
nucleus
(MRN)
input
modulates
medial
prefrontal
cortex
(mPFC),
a
critical
component
fear
circuitry.
show
that
vesicular
glutamate
transporter
3
(VGLUT3)–expressing
MRN
activate
VGLUT3-
and
somatostatin-expressing
in
mPFC.
Consistent
with
this
modulation
mPFC
GABAergic
neurons,
activation
(VGLUT3)
enhances
transmission
pyramidal
attenuates
memory
female
but
not
male
mice.
Serotonin
plays
key
role
neuron–mediated
plasticity
In
agreement
female-specific
effects,
we
observed
sex
differences
onto
dual
release
GABA.
Our
results
demonstrate
cell
type–specific
by
reveal
sex-specific
neuromodulation
synaptic
plasticity.
Fundamental Research,
Год журнала:
2024,
Номер
5(1), С. 93 - 102
Опубликована: Фев. 5, 2024
Neural
information
transmission
between
deep
brain
nuclei
and
the
cortex
is
essential
for
function.
Currently,
high-resolution
simultaneous
detection
of
neural
large-scale
still
poses
challenges.
We
have
developed
microelectrode
arrays
based
on
Micro-Electro-Mechanical
System
technology,
modified
electrode
surface
with
nanomaterials
to
improve
performance.
This
study
combined
extended-field-of-view
microscopy
achieve
recording
claustrum
(CLA)
electrophysiology
wide-field
cortical
calcium
imaging
at
single-cell
resolution.
work
investigated
synchronous
changes
in
CLA
mice
during
whole
process
from
wakefulness
anesthesia
then
wakefulness,
summarized
characteristics
signaling
under
different
inhalation
concentrations.
found
synergy
microscopic
spike
local
field
potential
neurons
anesthesia,
law
that
high
concentration
enhanced
synchronization
cortex.
The
combination
also
gives
a
new
method
multimodal
multi-brain
region
information.
