Gradient scaffolds for osteochondral tissue engineering and regeneration
Ziqi Xiong,
Fangyuan Hong,
Zhonglin Wu
и другие.
Chemical Engineering Journal,
Год журнала:
2024,
Номер
498, С. 154797 - 154797
Опубликована: Авг. 13, 2024
Язык: Английский
Human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) for modeling cardiac arrhythmias: strengths, challenges and potential solutions
Jyotsna Joshi,
C. Albers,
Nathan Smole
и другие.
Frontiers in Physiology,
Год журнала:
2024,
Номер
15
Опубликована: Сен. 12, 2024
Ion
channels
and
cytoskeletal
proteins
in
the
cardiac
dyad
play
a
critical
role
maintaining
excitation-contraction
(E-C)
coupling
provide
homeostasis.
Functional
changes
these
proteins,
whether
induced
by
genetic,
epigenetic,
metabolic,
therapeutic,
or
environmental
factors,
can
disrupt
normal
electrophysiology,
leading
to
abnormal
E-C
arrhythmias.
Animal
models
heterologous
cell
cultures
platforms
elucidate
pathogenesis
of
arrhythmias
for
basic
research;
however,
traditional
systems
do
not
truly
reflect
human
electro-pathophysiology.
Notably,
patients
with
same
genetic
variants
inherited
channelopathies
(ICC)
often
exhibit
incomplete
penetrance
variable
expressivity
which
underscores
need
establish
patient-specific
disease
comprehend
mechanistic
pathways
determine
personalized
therapies.
Patient-specific
pluripotent
stem
cell-derived
cardiomyocytes
(iPSC-CMs)
inherit
background
patient
electrophysiological
characteristics
native
cardiomyocytes.
Thus,
iPSC-CMs
an
innovative
translational
pivotal
platform
modeling
therapeutic
screening.
In
this
review,
we
will
examine
how
historically
evolved
model
arrhythmia
syndromes
dish,
their
utility
understanding
specific
ion
functional
causing
We
also
CRISPR/Cas9
have
enabled
establishment
patient-independent
variant-induced
iPSC-CMs-based
models.
Next,
limitations
using
respect
Язык: Английский
Autologous iPSC- and MSC-derived chondrocyte implants for cartilage repair in a miniature pig model
Stem Cell Research & Therapy,
Год журнала:
2025,
Номер
16(1)
Опубликована: Фев. 23, 2025
Язык: Английский
Cell reprogramming: methods, mechanisms and applications
Cell Regeneration,
Год журнала:
2025,
Номер
14(1)
Опубликована: Март 27, 2025
Abstract
Cell
reprogramming
represents
a
powerful
approach
to
achieve
the
conversion
cells
of
one
type
into
another
interest,
which
has
substantially
changed
landscape
in
field
developmental
biology,
regenerative
medicine,
disease
modeling,
drug
discovery
and
cancer
immunotherapy.
is
complex
ordered
process
that
involves
coordination
transcriptional,
epigenetic,
translational
metabolic
changes.
Over
past
two
decades,
range
questions
regarding
facilitators/barriers,
trajectories,
mechanisms
cell
have
been
extensively
investigated.
This
review
summarizes
recent
advances
mediated
by
transcription
factors
or
chemical
molecules,
followed
elaborating
on
important
roles
biophysical
cues
reprogramming.
Additionally,
this
will
detail
our
current
understanding
govern
reprogramming,
including
involvement
recently
discovered
biomolecular
condensates.
Finally,
discusses
broad
applications
future
directions
development,
regenerative/rejuvenation
therapy,
Язык: Английский
TGFβ family signaling in human stem cell self-renewal and differentiation
Cell Regeneration,
Год журнала:
2024,
Номер
13(1)
Опубликована: Ноя. 28, 2024
Abstract
Human
stem
cells
are
undifferentiated
with
the
capacity
for
self-renewal
and
differentiation
into
distinct
cell
lineages,
playing
important
role
in
development
maintenance
of
diverse
tissues
organs.
The
microenvironment
provides
crucial
factors
components
that
exert
significant
influence
over
determination
fate.
Among
these
factors,
cytokines
from
transforming
growth
factor
β
(TGFβ)
superfamily,
including
TGFβ,
bone
morphogenic
protein
(BMP),
Activin
Nodal,
have
been
identified
as
regulators
governing
differentiation.
In
this
review,
we
present
a
comprehensive
overview
pivotal
roles
played
by
TGFβ
superfamily
signaling
human
embryonic
cells,
somatic
induced
pluripotent
cancer
cells.
Furthermore,
summarize
latest
research
advancements
family
various
cell-based
therapy,
discussing
their
potential
clinical
applications
therapy
regeneration
medicine.
Язык: Английский
Role of the TGF-β signaling pathway in induced pluripotent stem cells reprogramming
Chinese Medical Journal,
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 22, 2024
To
the
Editor:
The
discovery
of
induced
pluripotent
stem
cells
(iPSCs)
by
Takahashi
and
Yamanaka
in
2006
was
hailed
as
a
major
breakthrough
cell
research.
And
large
number
experimental
studies
have
proven
that
transforming
growth
factor-β
(TGF-β)
signaling
pathway
is
key
iPSC
reprogramming.
TGF-β
can
promote
or
inhibit
reprogramming
under
different
conditions
[Figure
1].
This
article
describes
role
reprogramming.Figure
1:
conditions.
Akt:
protein
kinase
B;
BMP:
Bone
Morphogenetic
Protein;
Erk:
extracellular
signal-regulated
kinase;
GRB2:
factor
receptor-bound
2;
iPSC:
cell;
JNK:
c-Jun
N-terminal
Kinase;
Klf4:
kruppel-like
4;
LIMK:
Lim-kinase;
MEK:
mitogen-activated
MEKK1:
1;
MIS:
Müllerian
inhibiting
substance;
MKK:MAP
MLC:
myosin
light
chain;
MLK3:
mixed
lineage
3;
mTOR:
mechanistic
target
rapamycin;
Oct4:
octamer-binding
transcription
PI3K:
phosphatidylinositol
3-kinase;
p38:
p38
Raf:
rapidly
accelerated
fibrosarcoma;
Ras:
rat
sarcoma;
RhoA:
ras
homolog
gene
family
member
A;
ROCK:
Rho-associated
ShcA:
Src
homology
2
domain-containing
SOS:
son
sevenless;
Sox2:
SRY-box
S6K:
S6
TAK1:
beta-activated
TGF-β:
Transforming
factor-β.According
to
homology,
superfamily
be
divided
into
three
subfamilies:
various
subtypes
(β1,
β2,
β3),
activin,
bone
morphogenetic
(BMP).
transmits
signals
through
receptors,
which
type
I
receptors
(TβRI)/activin
receptor-like
(ALK5),
II
(TβRII),
III
(TβRIII)
according
their
structural
functional
properties.
TβRI
TβRII
complex
required
for
signal
transduction.
Upon
binding
ligand,
catalyzes
phosphorylation
serine
threonine
residues
guanidine
specificity
(GS)
region
TβRI,
resulting
group
downstream
molecules
called
Smads
cell.
Smad
plus
general
(GTF),
other
factors,
supplementary
targets
transcription.
In
addition,
TGF-β/BMP
cytokines
also
activate
non-Smad
molecules.
For
example,
activated
induces
formation
A
(ShcA)/growth
(GRB2)/son
sevenless
(SOS)
phosphorylating
ShcA
further
activates
(ERK)/mitogen-activated
(MAPK)
Rat
sarcoma
(Ras)-rapidly
fibrosarcoma
(Raf)-Mitogen-activated
(MEK)
pathway;
receptor
TNF
associated
6
(TRAF6)
catalyze
1
(TAK1)
undergo
K63-linked
polyubiquitination,
then
(JNK)
(p38
MAPK)
via
MAP
(MKK).
induce
its
bind
p85,
regulatory
subunit
phosphoinositide
3-kinase
(PI3K),
PI3K-protein
B
(Akt)-mechanistic
rapamycin
(mTOR)
transcriptional
regulation.
Activated
AKT
epithelial-mesenchymal
transition
(EMT)
regulation
ribonucleoprotein
E1.
affects
activity
EMT-triggering
pathways,
such
Notch,
Wnt,
integrin
pathways.
Meanwhile,
TGF-β-induced
regulate
signaling.
regulates
differentiation.
TGF-β1
has
been
shown
transformation
human-induced
(hiPSCs)
-derived
neural
(NSCs)
neurons
astrocytes
vitro.
optogenetic
modulation
precisely
control
differentiation
hiPSCs
mesenchymal
lineage.[1]
It
hydrodynamic
stress
generated
shaking
culture
stimulates
chondrogenically
iPSCs
(CI-iPSCs),
promoting
chondrogenic
mouse
Inhibition
ability.
RepSox
displaces
(Sox2)
ubiquitously
expressed
cultures
containing
stable
intermediate
captured
partially
reprogrammed
state.
process,
turn,
leads
sustained
Nanog
gene,
absence
Sox2.[2]
Moreover,
Misaki
Yamashita
et
al[3]
found
inhibition
could
human
iPSC-derived
brain
microvascular
endothelial-like
(iBMELCs)
increase
proportion
endothelial
differentiated
population.
MEK,
DNMT,
effective
inducing
enterocytes.
Human
noncardiomyocyte
cardiac
(hiPSC-NMCCs)
differentiate
myofibroblast-like
when
cultured
with
transplanted
infarcted
hearts.[4]
Gong
al[5]
achieve
directed
hiPSCs-derived
crest
(NCCs)
using
chemically
defined
media
glycogen
synthesis
(GSK-3)
inhibitors
inhibitors.
Indeed,
essential
maintaining
pluripotency
(hPSCs).
Single-cell
RNA
sequencing
performed
Mabrouk
al[6]
revealed
important
functions
maintenance
during
early
embryonic
stages.
hPSC,
regulator
ZNF398
mediates
epithelial
properties
TGF-β.
Mechanistically,
binds
active
promoter
enhancer
SMAD3
histone
acetyltransferase
EP300,
enabling
transcription.[7]
context
somatic
reprogramming,
disrupts
activation
well
colony
formation.[7]
hESF
9
medium
absolutely
dependent
on
presence
TGF-β1.
Activin
Nodal
play
roles
networks.
PI3K
activation,
EMT/AKT
establishes
where
activin
A/SMAD2/3
stimulate
self-renewal
activating
genes,
including
NANOG.
Singh
al[8]
PI3K/AKT
inhibited
RAF/MEK/ERK
typical
Wnt
allowing
SMAD2/3
pluripotency-related
genes
NANOG
high
level
undifferentiated
state.[8]
inhibits
mesenchymal-epithelial
(MET),
an
event
fibroblasts
iPSCs.
provides
possible
mechanism
generation
Small
targeting
MET
machinery,
(SB431542),
MEK
(PD0325901),
ROCK
(thiazovivin),
alone
combination,
significantly
increased
efficiency.
According
current
studies,
BMP
plays
reprogramming:
BMP7
percentage
colonies
four
OSKM
(OCT4,
KLF4,
SOX2,
c-Myc)
(MEFs).[9]
Inhibiting
enhanced
efficiency
kinetics
OSKM-reprogrammed
MEFs,
while
prevented
Considering
inhibitor
treatment
most
stages
production,
it
believed
exert
synergistic
effect
factors
rather
than
fibroblast
transformation.
Besides,
evidenced
therapy
replace
solitary
C-MYC
suggests
acts
bypassing
programming
SOX2
entirely.
Ruetz
al[10]
showed
constitutive
increases
thereby
serving
enhance
factor-mediated
Studies
Math6
counteract
signaling,
affecting
initiating
steps
cellular
However,
there
are
still
many
limitations
research
specific
pathway.
instance,
remain
elusive;
this
undergoes
dynamic
changes
response
conditions;
unidentified
involved
unknown
potential
understanding
participation
modulating
conclusion,
process
exploring
mechanism,
expression
changed
significantly.
mechanisms
studied.
Funding
work
supported
grants
from
Key
Projects
Yunnan
Province
(No.
202301AY070001-034)
Project
Provincial
Department
Education
2023Y0827).
Conflicts
interest
None.
Язык: Английский
Optogenetics and Its Transformative Role in Tissue Engineering and Regenerative Medicine
Nano Select,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 27, 2024
ABSTRACT
Optogenetics,
a
revolutionary
technique
leveraging
light
stimulation
for
precise
cellular
control,
holds
immense
potential
in
regenerative
medicine.
Offering
unparalleled
spatial
and
temporal
accuracy,
the
entrance
of
optogenetics
into
tissue
engineering
medicine
(TERM)
empowers
researchers
to
modify
genes
precisely
reversibly,
control
signal
pathways
antibodies,
as
well
alter
biomaterial
properties.
Optogenetics
provides
unprecedented
manipulation
physiological
regeneration,
replication
intricate
developmental
processes,
laboratory
setting.
Although
further
investigation
is
required
safe
feasible
injection
optogenetic
systems
human
bodies,
use
has
already
led
great
amount
research
on
several
tissues
systems.
It
found
diverse
applications
TERM
skin
connective
tissue,
endothelium,
bone,
cartilage,
muscle;
with
over
vitro
or
vivo
production
these
tissues,
critical
proteins
pathways,
creation
light‐controlled
wound
coverings
even
tool
directional
growth
living
subjects.
emerges
transformative
force
shaping
future
medical
science,
demonstrating
pivotal
role
advancing
paving
way
innovative
therapeutic
strategies.
Язык: Английский
Recent advancement of sonogenetics: A promising noninvasive cellular manipulation by ultrasound
Genes & Diseases,
Год журнала:
2023,
Номер
11(5), С. 101112 - 101112
Опубликована: Сен. 15, 2023
Recent
advancements
in
biomedical
research
have
underscored
the
importance
of
noninvasive
cellular
manipulation
techniques.
Sonogenetics,
a
method
that
uses
genetic
engineering
to
produce
ultrasound-sensitive
proteins
target
cells,
is
gaining
prominence
along
with
optogenetics,
electrogenetics,
and
magnetogenetics.
Upon
stimulation
ultrasound,
these
trigger
cascade
activities
functions.
Unlike
traditional
ultrasound
modalities,
sonogenetics
offers
enhanced
spatial
selectivity,
improving
precision
safety
disease
treatment.
This
technology
broadens
scope
non-surgical
interventions
across
wide
range
clinical
therapeutic
applications,
including
neuromodulation,
oncologic
treatments,
stem
cell
therapy,
beyond.
Although
current
literature
predominantly
emphasizes
ultrasonic
this
review
comprehensive
exploration
sonogenetics.
We
discuss
properties,
specific
employed
sonogenetics,
technique's
potential
managing
conditions
such
as
neurological
disorders,
cancer,
ophthalmic
diseases,
therapies.
Our
objective
stimulate
fresh
perspectives
for
further
promising
field.
Язык: Английский
Optogenetic manipulation of BMP signaling to drive chondrogenic differentiation of hPSCs
Cell Reports,
Год журнала:
2023,
Номер
42(12), С. 113502 - 113502
Опубликована: Ноя. 29, 2023
Optogenetics
is
a
rapidly
advancing
technology
combining
photochemical,
optical,
and
synthetic
biology
to
control
cellular
behavior.
Together,
sensitive
light-responsive
optogenetic
tools
human
pluripotent
stem
cell
differentiation
models
have
the
potential
fine-tune
unpick
processes
by
which
specification
tissue
patterning
are
controlled
morphogens.
We
used
an
bone
morphogenetic
protein
(BMP)
signaling
system
(optoBMP)
drive
chondrogenic
of
embryonic
cells
(hESCs).
engineered
light-sensitive
hESCs
through
CRISPR-Cas9-mediated
integration
optoBMP
into
AAVS1
locus.
The
activation
with
blue
light,
in
lieu
BMP
growth
factors,
resulted
mechanisms
upregulation
phenotype,
significant
transcriptional
differences
compared
dark.
Furthermore,
differentiated
light
could
form
pellets
consisting
hyaline-like
cartilaginous
matrix.
Our
findings
indicate
applicability
optogenetics
for
understanding
development
engineering.
Язык: Английский