Comparative single-cell transcriptomic atlases of drosophilid brains suggest glial evolution during ecological adaptation
PLoS Biology,
Год журнала:
2025,
Номер
23(4), С. e3003120 - e3003120
Опубликована: Апрель 29, 2025
To
explore
how
brains
change
upon
species
evolution,
we
generated
single-cell
transcriptomic
atlases
of
the
central
three
closely
related
but
ecologically
distinct
drosophilids:
generalists
Drosophila
melanogaster
and
simulans
,
noni
fruit
specialist
sechellia
.
The
global
cellular
composition
these
species’
is
well-conserved,
predicted
a
few
cell
types
with
different
frequencies,
notably
perineurial
glia
blood–brain
barrier,
which
validate
in
vivo
Gene
expression
analysis
revealed
that
evolve
at
rates
patterns,
glial
populations
exhibiting
greatest
divergence
between
species.
Compared
to
D.
brain,
gene
patterns
are
more
divergent
than
—despite
their
similar
phylogenetic
distance
from
—indicating
specialization
reflected
structure
function
its
brain.
Expression
changes
include
several
metabolic
signaling
genes,
suggestive
adaptations
novel
source
nutrition.
Additional
on
genes
responsive
dietary
supplement
noni,
pointing
as
sites
for
both
physiological
genetic
adaptation
this
fruit.
Our
represent
first
comparative
datasets
“whole”
provide
comprehensive
foundation
studying
evolvability
nervous
systems
well-defined
ecological
framework.
Язык: Английский
Age-dependent switched taste behavior to ribose
Insect Biochemistry and Molecular Biology,
Год журнала:
2024,
Номер
174, С. 104194 - 104194
Опубликована: Окт. 13, 2024
Язык: Английский
Acid and Alkali Taste Sensation
Metabolites,
Год журнала:
2023,
Номер
13(11), С. 1131 - 1131
Опубликована: Ноя. 4, 2023
Living
organisms
rely
on
pH
levels
for
a
multitude
of
crucial
biological
processes,
such
as
the
digestion
food
and
facilitation
enzymatic
reactions.
Among
these
organisms,
animals,
including
insects,
possess
specialized
taste
organs
that
enable
them
to
discern
between
acidic
alkaline
substances
present
in
their
sources.
This
ability
is
vital,
compounds
directly
influences
both
nutritional
value
overall
health
impact
ingested
substances.
In
response
various
chemical
properties
naturally
occurring
compounds,
insects
have
evolved
peripheral
organs.
These
sensory
structures
play
pivotal
role
identifying
distinguishing
nourishing
potentially
harmful
foods.
this
concise
review,
we
aim
provide
an
in-depth
examination
molecular
mechanisms
governing
pH-dependent
responses,
encompassing
stimuli,
within
fruit
fly,
Drosophila
melanogaster,
drawing
insights
from
comprehensive
analysis
existing
research
articles.
Язык: Английский
Genome-wide identification and molecular evolution of elongation family of very long chain fatty acids proteins in Cyrtotrachelus buqueti
BMC Genomics,
Год журнала:
2024,
Номер
25(1)
Опубликована: Авг. 2, 2024
To
reveal
the
molecular
function
of
elongation
family
very
long
chain
fatty
acids(ELO)
protein
in
Cyrtotrachelus
buqueti,
we
have
identified
15
ELO
proteins
from
C.buqueti
genome.
CbuELO
were
located
on
four
chromosomes.
Their
isoelectric
points
ranged
9.22
to
9.68,
and
they
alkaline.
These
stable
hydrophobic.
had
transmembrane
movement,
multiple
phosphorylation
sites.
The
secondary
structure
was
mainly
α-helix.
A
total
10
conserved
motifs
family.
Phylogenetic
analysis
showed
that
evolutionary
relationships
between
C.
buqueti
Tribolium
castaneum
closest.
Developmental
transcriptome
indicated
CbuELO10,
CbuELO13
CbuELO02
genes
key
enzyme
determine
synthesis
acids
pupae
eggs,
CbuELO6
CbuELO7
male,
CbuELO8
CbuELO11
larva.
Transcriptome
under
different
temperature
conditions
CbuELO1,
CbuELO5,
CbuELO12
CbuELO14
participated
regulating
stress
responses.
at
feeding
times
gene
expression
level
all
time
periods
significant
downregulation.
qRT-PCR
experiment
verified
changes
conditions.
Protein-protein
interaction
9
related
each
other,
CbuELO4
more
than
one
relationship.
results
lay
a
theoretical
foundation
for
further
studying
its
during
growth
development
buqueti.
Язык: Английский
Capsaicin Responses in Drosophila: Exploring a Non-Trpv1 Model
Опубликована: Янв. 1, 2024
Язык: Английский
Evolution of taste processing shifts dietary preference
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 12, 2024
Abstract
Food
choice
is
an
important
driver
of
speciation
and
invasion
novel
ecological
niches.
However,
we
know
little
about
the
mechanisms
leading
to
changes
in
dietary
preference.
Here,
use
three
closely-related
species
Drosophila
sechellia
,
D.
simulans
melanogaster
study
taste
circuit
food
evolution.
sechellia,
a
host
specialist,
feeds
exclusively
on
single
fruit
(
Morinda
citrifolia
noni)
-
latter
two
are
generalists
living
various
substrates.
Using
quantitative
feeding
assays,
recapitulate
preference
for
noni
detect
conserved
sweet
but
altered
bitter
sensitivity
via
calcium
imaging
peripheral
neurons.
Noni
surprisingly
activates
sensing
neurons
more
strongly
due
small
deletion
one
gustatory
receptor.
volumetric
ventral
brain,
show
that
instead
physiology,
species-specific
processing
sugar
signals
sensorimotor
circuits
recapitulates
differences
Our
data
support
receptor
alone
cannot
explain
rather
modifications
how
sensory
information
transformed
into
motor
commands.
Язык: Английский
The gustatory receptor BdorGr43a mediated sucrose preference in the feeding of Bactrocera dorsalis
International Journal of Biological Macromolecules,
Год журнала:
2024,
Номер
unknown, С. 136774 - 136774
Опубликована: Окт. 1, 2024
Язык: Английский
Comparative single-cell transcriptomic atlases reveal conserved and divergent features of drosophilid central brains
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Ноя. 23, 2023
Abstract
To
explore
how
brains
change
upon
species
evolution,
we
generated
single-cell
transcriptomic
atlases
of
the
central
three
closely-related
but
ecologically-distinct
drosophilids:
generalists
Drosophila
melanogaster
and
simulans
,
noni
fruit
specialist
sechellia
.
The
global
cellular
composition
these
species’
is
well-conserved,
predicted
a
few
cell
types
(perineurial
glia,
sNPF
Dh44
peptidergic
neurons)
with
divergent
frequencies.
Gene
expression
analysis
revealed
that
distinct
within
brain
evolve
at
different
rates
patterns;
notably,
glial
exhibit
greatest
divergence
between
species.
Compared
to
D.
gene
patterns
in
display
greater
deviation
than
those
-
despite
their
similar
phylogenetic
distance
from
distinctive
ecological
specialization
reflected
structure
function
its
brain.
Expression
changes
encompass
metabolic
ecdysone
signaling
genes,
suggestive
adaptations
novel
demands.
Additional
on
genes
responsive
dietary
supplement
noni,
pointing
glia
as
sites
for
both
physiological
genetic
adaptation
conditions.
Our
represent
first
comparative
analyses
“whole”
brains,
provide
comprehensive
foundation
studying
evolvability
nervous
systems
well-defined
framework.
Язык: Английский