Comparative single-cell transcriptomic atlases reveal conserved and divergent features of drosophilid central brains DOI Open Access
Daehan Lee, Richard Benton

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 23, 2023

Abstract To explore how brains change upon species evolution, we generated single-cell transcriptomic atlases of the central three closely-related but ecologically-distinct drosophilids: generalists Drosophila melanogaster and simulans , noni fruit specialist sechellia . The global cellular composition these species’ is well-conserved, predicted a few cell types (perineurial glia, sNPF Dh44 peptidergic neurons) with divergent frequencies. Gene expression analysis revealed that distinct within brain evolve at different rates patterns; notably, glial exhibit greatest divergence between species. Compared to D. gene patterns in display greater deviation than those - despite their similar phylogenetic distance from distinctive ecological specialization reflected structure function its brain. Expression changes encompass metabolic ecdysone signaling genes, suggestive adaptations novel demands. Additional on genes responsive dietary supplement noni, pointing glia as sites for both physiological genetic adaptation conditions. Our represent first comparative analyses “whole” brains, provide comprehensive foundation studying evolvability nervous systems well-defined framework.

Language: Английский

Comparative single-cell transcriptomic atlases of drosophilid brains suggest glial evolution during ecological adaptation DOI Creative Commons
Daehan Lee, Michael P. Shahandeh,

Liliane Abuin

et al.

PLoS Biology, Journal Year: 2025, Volume and Issue: 23(4), P. e3003120 - e3003120

Published: April 29, 2025

To explore how brains change upon species evolution, we generated single-cell transcriptomic atlases of the central three closely related but ecologically distinct drosophilids: generalists Drosophila melanogaster and simulans , noni fruit specialist sechellia . The global cellular composition these species’ is well-conserved, predicted a few cell types with different frequencies, notably perineurial glia blood–brain barrier, which validate in vivo Gene expression analysis revealed that evolve at rates patterns, glial populations exhibiting greatest divergence between species. Compared to D. brain, gene patterns are more divergent than —despite their similar phylogenetic distance from —indicating specialization reflected structure function its brain. Expression changes include several metabolic signaling genes, suggestive adaptations novel source nutrition. Additional on genes responsive dietary supplement noni, pointing as sites for both physiological genetic adaptation this fruit. Our represent first comparative datasets “whole” provide comprehensive foundation studying evolvability nervous systems well-defined ecological framework.

Language: Английский

Citations

0

Age-dependent switched taste behavior to ribose DOI
Jiun Sang, Youngseok Lee

Insect Biochemistry and Molecular Biology, Journal Year: 2024, Volume and Issue: 174, P. 104194 - 104194

Published: Oct. 13, 2024

Language: Английский

Citations

2

Acid and Alkali Taste Sensation DOI Creative Commons
Prakash Pandey, Bhanu Shrestha, Youngseok Lee

et al.

Metabolites, Journal Year: 2023, Volume and Issue: 13(11), P. 1131 - 1131

Published: Nov. 4, 2023

Living organisms rely on pH levels for a multitude of crucial biological processes, such as the digestion food and facilitation enzymatic reactions. Among these organisms, animals, including insects, possess specialized taste organs that enable them to discern between acidic alkaline substances present in their sources. This ability is vital, compounds directly influences both nutritional value overall health impact ingested substances. In response various chemical properties naturally occurring compounds, insects have evolved peripheral organs. These sensory structures play pivotal role identifying distinguishing nourishing potentially harmful foods. this concise review, we aim provide an in-depth examination molecular mechanisms governing pH-dependent responses, encompassing stimuli, within fruit fly, Drosophila melanogaster, drawing insights from comprehensive analysis existing research articles.

Language: Английский

Citations

4

Genome-wide identification and molecular evolution of elongation family of very long chain fatty acids proteins in Cyrtotrachelus buqueti DOI Creative Commons
Chun Fu, Ting Yang,

Hong Liao

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Aug. 2, 2024

To reveal the molecular function of elongation family very long chain fatty acids(ELO) protein in Cyrtotrachelus buqueti, we have identified 15 ELO proteins from C.buqueti genome. CbuELO were located on four chromosomes. Their isoelectric points ranged 9.22 to 9.68, and they alkaline. These stable hydrophobic. had transmembrane movement, multiple phosphorylation sites. The secondary structure was mainly α-helix. A total 10 conserved motifs family. Phylogenetic analysis showed that evolutionary relationships between C. buqueti Tribolium castaneum closest. Developmental transcriptome indicated CbuELO10, CbuELO13 CbuELO02 genes key enzyme determine synthesis acids pupae eggs, CbuELO6 CbuELO7 male, CbuELO8 CbuELO11 larva. Transcriptome under different temperature conditions CbuELO1, CbuELO5, CbuELO12 CbuELO14 participated regulating stress responses. at feeding times gene expression level all time periods significant downregulation. qRT-PCR experiment verified changes conditions. Protein-protein interaction 9 related each other, CbuELO4 more than one relationship. results lay a theoretical foundation for further studying its during growth development buqueti.

Language: Английский

Citations

1

Capsaicin Responses in Drosophila: Exploring a Non-Trpv1 Model DOI
Gerardo Flores-Iga,

Mohankumar Amirthalingam,

Carlos E. Ortíz

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0

Evolution of taste processing shifts dietary preference DOI Creative Commons
Enrico Bertolini, Daniel Münch,

Justine Pascual

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 12, 2024

Abstract Food choice is an important driver of speciation and invasion novel ecological niches. However, we know little about the mechanisms leading to changes in dietary preference. Here, use three closely-related species Drosophila sechellia , D. simulans melanogaster study taste circuit food evolution. sechellia, a host specialist, feeds exclusively on single fruit ( Morinda citrifolia noni) - latter two are generalists living various substrates. Using quantitative feeding assays, recapitulate preference for noni detect conserved sweet but altered bitter sensitivity via calcium imaging peripheral neurons. Noni surprisingly activates sensing neurons more strongly due small deletion one gustatory receptor. volumetric ventral brain, show that instead physiology, species-specific processing sugar signals sensorimotor circuits recapitulates differences Our data support receptor alone cannot explain rather modifications how sensory information transformed into motor commands.

Language: Английский

Citations

0

The gustatory receptor BdorGr43a mediated sucrose preference in the feeding of Bactrocera dorsalis DOI

Bao Dong,

Hongfei Li,

Huai-Wang Guo

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 136774 - 136774

Published: Oct. 1, 2024

Language: Английский

Citations

0

Comparative single-cell transcriptomic atlases reveal conserved and divergent features of drosophilid central brains DOI Open Access
Daehan Lee, Richard Benton

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 23, 2023

Abstract To explore how brains change upon species evolution, we generated single-cell transcriptomic atlases of the central three closely-related but ecologically-distinct drosophilids: generalists Drosophila melanogaster and simulans , noni fruit specialist sechellia . The global cellular composition these species’ is well-conserved, predicted a few cell types (perineurial glia, sNPF Dh44 peptidergic neurons) with divergent frequencies. Gene expression analysis revealed that distinct within brain evolve at different rates patterns; notably, glial exhibit greatest divergence between species. Compared to D. gene patterns in display greater deviation than those - despite their similar phylogenetic distance from distinctive ecological specialization reflected structure function its brain. Expression changes encompass metabolic ecdysone signaling genes, suggestive adaptations novel demands. Additional on genes responsive dietary supplement noni, pointing glia as sites for both physiological genetic adaptation conditions. Our represent first comparative analyses “whole” brains, provide comprehensive foundation studying evolvability nervous systems well-defined framework.

Language: Английский

Citations

1