Microbial Biotechnology,
Год журнала:
2025,
Номер
18(6)
Опубликована: Май 30, 2025
ABSTRACT
The
gut
microbiome
plays
a
crucial
role
in
maintaining
intestinal
homeostasis
and
influencing
immune‐mediated
diseases.
Human
faecal
microbiota
transplantation
(FMT)
is
often
employed
murine
models
to
investigate
the
of
human
microbes
disease
regulation,
but
methods
for
effective
colonisation
require
refinement.
This
study
aimed
assess
efficiency
model
using
FMT
with
faeces,
focusing
particularly
on
impact
depletion
via
polyethylene
glycol
(PEG)
comparing
oral‐gastric
gavage
enema
administration
routes.
Our
findings
revealed
that
PEG‐induced
enhanced
mice.
Oral‐gastric
prolonged
colonisation,
while
facilitated
quicker
resolution
dysbiosis,
both
inducing
selective
microbial
time‐dependent
manner.
Notably,
genera
such
as
Bacteroides
,
Blautia
Medicaternibacter
Bifidobacteria
were
successfully
colonised,
whereas
Roseburia
Anaerostipes
Anaerobutyricum
Faecalibacterium
failed
establish
post‐FMT.
These
highlight
challenges
replicating
underscore
importance
selecting
appropriate
based
desired
outcomes.
provides
valuable
insights
into
dynamics
mice,
contributing
development
more
strategies
treatment.
Gastroenterology,
Год журнала:
2024,
Номер
166(3), С. 409 - 434
Опубликована: Фев. 21, 2024
Fecal
microbiota-based
therapies
include
conventional
fecal
microbiota
transplant
and
US
Food
Drug
Administration-approved
therapies,
live-jslm
spores
live-brpk.
The
American
Gastroenterological
Association
(AGA)
developed
this
guideline
to
provide
recommendations
on
the
use
of
in
adults
with
recurrent
Clostridioides
difficile
infection;
severe
fulminant
C
inflammatory
bowel
diseases,
including
pouchitis;
irritable
syndrome.
Clinical Microbiology Reviews,
Год журнала:
2024,
Номер
37(2)
Опубликована: Май 8, 2024
SUMMARYGiven
the
importance
of
gut
microbial
homeostasis
in
maintaining
health,
there
has
been
considerable
interest
developing
innovative
therapeutic
strategies
for
restoring
microbiota.
One
such
approach,
fecal
microbiota
transplantation
(FMT),
is
main
"whole
microbiome
replacement"
strategy
and
integrated
into
clinical
practice
guidelines
treating
recurrent
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Фев. 21, 2024
The
bidirectional
communication
between
the
gut
and
brain
or
gut-brain
axis
is
regulated
by
several
microbes
microbial
derived
metabolites,
such
as
short-chain
fatty
acids,
trimethylamine
N-oxide,
lipopolysaccharides.
Gut
microbiota
(GM)
produce
neuroactives,
specifically
neurotransmitters
that
modulates
local
central
neuronal
functions.
An
imbalance
intestinal
commensals
pathobionts
leads
to
a
disruption
in
dysbiosis,
which
affects
barrier
integrity
gut-immune
neuroimmune
systems.
Currently,
fecal
transplantation
(FMT)
recommended
for
treatment
of
recurrent
ABSTRACT
Dysbiosis
refers
to
the
disruption
of
gut
microbiota
balance
and
is
pathological
basis
various
diseases.
The
main
pathogenic
mechanisms
include
impaired
intestinal
mucosal
barrier
function,
inflammation
activation,
immune
dysregulation,
metabolic
abnormalities.
These
involve
dysfunctions
in
gut–brain
axis,
gut–liver
others
cause
broader
effects.
Although
association
between
diseases
caused
by
dysbiosis
has
been
extensively
studied,
many
questions
remain
regarding
specific
treatment
strategies.
This
review
begins
examining
causes
summarizes
potential
representative
imbalance.
It
integrates
clinical
evidence
explore
preventive
therapeutic
strategies
targeting
emphasizing
importance
understanding
dysbiosis.
Finally,
we
summarized
development
artificial
intelligence
(AI)
research
suggested
that
it
will
play
a
critical
role
future
studies
on
combining
multiomics
technologies
AI
further
uncover
complex
drive
personalized
Abstract
Background
Fecal
microbiota
transplantation
(FMT)
is
a
therapeutic
intervention
used
to
treat
diseases
associated
with
the
gut
microbiome.
In
human
microbiome,
phages
have
been
implicated
in
influencing
health,
successful
engraftment
of
donor
correlated
FMT
treatment
efficacy.
The
impact
that
gastrointestinal
exert
on
health
has
primarily
connected
their
ability
modulate
bacterial
communities
gut.
Nonetheless,
how
affects
recipients’
phage
populations,
and
turn,
this
influences
environment,
not
yet
fully
understood.
study,
we
investigated
effects
phageome
composition
participants
within
Gut
Bugs
Trial
(GBT),
double-blind,
randomized,
placebo-controlled
trial
efficacy
treating
obesity
comorbidities
adolescents.
Stool
samples
collected
from
donors
at
time
recipients
four
points
(i.e.,
baseline
6
weeks,
12
26
weeks
post-intervention),
underwent
shotgun
metagenomic
sequencing.
Phage
sequences
were
identified
characterized
silico
examine
evidence
assess
extent
FMT-induced
alterations
composition.
Results
Donor
engrafted
stably
following
FMT,
composing
significant
proportion
for
entire
course
study
(33.8
±
1.2%
females
33.9
3.7%
males).
varied
between
was
positively
alpha
diversity.
caused
shift
toward
donors’
increased
diversity
variability
over
time.
Conclusions
significantly
altered
recipients'
and,
overall,
microbial
populations.
increase
consistent
population
dynamics.
This
proposes
play
critical
role
modulating
environment
suggests
novel
approaches
understanding
altering
recipient’s
registration
registered
Australian
New
Zealand
Clinical
Trials
Registry
(ACTR
N12615001351505).
protocol:
protocol
available
https://bmjopen.bmj.com/content/9/4/e026174
.
