Journal of Pharmaceutical Analysis,
Год журнала:
2022,
Номер
12(5), С. 701 - 710
Опубликована: Май 17, 2022
With
the
modernization
and
internationalization
of
traditional
Chinese
medicine
(TCM),
requirement
for
quality
control
has
increased.
The
marker
(Q-marker)
is
an
important
standard
in
this
field
been
implemented
with
remarkable
success
recent
years.
However,
establishment
Q-markers
remains
fragmented
process
lacks
systematicity,
resulting
inconsistent
insufficient
correlation
clinical
efficacy
safety
TCM.
This
review
introduces
four
multimodal
integrated
approaches
that
contribute
to
discovery
more
comprehensive
accurate
Q-markers,
thus
aiding
new
patterns
based
on
characteristics
principles
These
include
whole-process
strategy,
chemical-activity-based
screening
method,
efficacy,
safety,
consistent
combination
TCM
theory-guided
approach.
Furthermore,
methodologies
representative
examples
these
strategies
are
described,
future
directions
questions
also
proposed.
Molecules,
Год журнала:
2023,
Номер
28(5), С. 2216 - 2216
Опубликована: Фев. 27, 2023
The
rhizome
of
Atractylodes
lancea
(RAL)
is
a
well-known
Chinese
herbal
medicine
(CHM)
that
has
been
applied
in
clinical
settings
for
thousands
years.
In
the
past
two
decades,
cultivated
RAL
gradually
replaced
wild
and
become
mainstream
practice.
quality
CHM
significantly
influenced
by
its
geographical
origin.
To
date,
limited
studies
have
compared
composition
from
different
origins.
As
essential
oil
primary
active
component
RAL,
strategy
combining
gas
chromatography-mass
spectrometry
(GC-MS)
chemical
pattern
recognition
was
first
to
compare
(RALO)
regions
China.
Total
ion
chromatography
(TIC)
revealed
RALO
origins
had
similar
composition;
however,
relative
content
main
compounds
varied
significantly.
addition,
26
samples
obtained
various
were
divided
into
three
categories
hierarchical
cluster
analysis
(HCA)
principal
(PCA).
Combined
with
location
analysis,
producing
classified
areas.
vary
depending
on
production
Furthermore,
one-way
variance
(ANOVA)
there
significant
differences
six
compounds,
including
modephene,
caryophyllene,
γ-elemene,
atractylon,
hinesol,
atractylodin,
between
Hinesol,
β-eudesmol
selected
as
potential
markers
distinguishing
areas
orthogonal
partial
least
squares
discriminant
(OPLS-DA).
conclusion,
GC-MS
this
research
identified
variations
across
developed
an
effective
method
geographic
origin
tracking
based
oils.
Molecules,
Год журнала:
2022,
Номер
27(20), С. 6944 - 6944
Опубликована: Окт. 16, 2022
Both
the
bulbs
and
flowers
of
Fritillaria
thunbergii
Miq.
(BFT
FFT)
are
widely
applied
as
expectorants
antitussives
in
traditional
Chinese
medicine,
but
few
studies
have
been
conducted
to
compare
chemical
compositions
these
plant
parts.
In
this
study,
50%
methanol
extracts
BFT
FFT
were
analyzed
via
UHPLC-Q-Exactive
Orbitrap
MS/MS,
feasibility
using
non-targeted
UHPLC-HRMS
metabolomics
molecular
networking
address
authentication
bulb
flower
samples
was
evaluated.
Principal
component
analysis
(PCA),
Orthogonal
Partial
Least
Squares-Discriminant
Analysis
(OPLS-DA),
heat
map
showed
there
dissimilar
metabolites
FFT.
As
a
result,
252
107
peaks
positive
ion
mode
negative
mode,
respectively,
considered
represent
significant
difference
variables
between
Then,
MS/MS-based
constructed
perform
an
in-depth
characterization
different
variables.
A
total
31
alkaloids
with
differences
annotated
paper,
including
seven
cis-D/E-vevanine
without
C20-OH
one
trans-D/E-cevanine
C20-OH,
thirteen
five
cevanine
N-oxide,
veratramine.
Among
alkaloids,
eight
had
higher
than
contents,
while
all
flavonoids
identified
our
work
greater
contents.
The
influence
ingredients
on
pharmacological
activities
should
be
investigated
future
studies.
