Analytical Sciences,
Год журнала:
2023,
Номер
39(6), С. 989 - 998
Опубликована: Фев. 24, 2023
In
this
paper,
an
amphiphilic
calix[8]arene
with
polyethylene
glycol
unit
branches
(C8A-PEG)
was
synthesized
and
applied
for
capillary
gas
chromatography
(GC).
The
C8A-PEG
coated
on
the
inner
wall
of
a
column
by
static
method
efficiency
3165
plates/m
polar
nature.
As
demonstrated,
has
excellent
physicochemical
properties
separation
performance
since
it
π-electron-rich
3D
cavity
which
combines
PEG
units.
Compared
two
columns
corresponding
to
construction
units
C8A
PEG,
shows
distinctly
advantageous
mixture
22
components
diverse
types.
Impressively,
satisfactory
resolution
positional
isomers
cis-/trans-
isomers,
especially
challenging
toluidine
dimethylaniline.
outstanding
distinguishing
capability
stationary
phase
is
mainly
attributed
abundant
molecular
recognition
interactions,
including
van
der
Waals,
dipole-dipole,
H-bonding
π-π
stacking
interactions.
This
work
proved
that
new
GC
phases
constructed
different
can
complement
each
other's
advantages,
improve
their
performance,
have
broad
application
prospects
in
chromatographic
analysis.
TrAC Trends in Analytical Chemistry,
Год журнала:
2023,
Номер
166, С. 117166 - 117166
Опубликована: Июль 8, 2023
Multi-dimensional
liquid
chromatography
(MD-LC)
continues
to
gain
in
popularity
for
applications
where
conventional
one-dimensional
is
insufficient
solve
the
analytical
problem
at
hand.
In
this
review
we
have
focused
on
articles
published
years
2019
early
2023
and
look
trends
using
our
previous
2018
as
a
baseline.
We
also
explored
usage
patterns
related
involvement
of
industrial
laboratories
research.
The
two
major
areas
technical
development
been
continued
work
modulation
strategies
that
help
mitigate
problems
associated
with
mobile
phase
mismatch
when
coupling
complementary
separation
mechanisms,
computer-aided
method
strategies.
Progress
these
making
2D-LC
easier
use,
it
appears
translating
shift
toward
more
by
laboratories.
Indeed,
over
34%
than
200
publications
last
four
had
least
one-industry
affiliated
author.
A
recent
inter-laboratory
comparison
study
performance
sophisticated
multi-stage,
multi-dimensional
therapeutic
protein
characterization
an
exemplary
indication
increasing
investment
MD-LC,
expect
trend
continue
foreseeable
future.
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(2), С. 1339 - 1353
Опубликована: Янв. 6, 2024
Two
offline
multidimensional
chromatography/high-resolution
mass
spectrometry
systems
(method
1:
fractionation
and
online
two-dimensional
liquid
chromatography,
2D-LC;
method
2:
2D-LC)
were
established
to
characterize
the
metabolites
simultaneously
from
three
Glycyrrhiza
species.
Ion
exchange
chromatography
in
first-dimensional
(1D)
separation
was
well
fractionated
between
acidic
(mainly
triterpenoids)
weakly
components
(flavonoids).
These
obtained
subsamples
got
sophisticated
by
second
(2D)
third
dimension
(3D)
of
either
reversed-phase
×
(RPC
RPC)
or
hydrophilic
interaction
RPC
(HILIC
RPC).
Orthogonality
for
2D/3D
separations
reached
0.73
1
0.81
2,
respectively.
We
could
1097
compounds
species
based
on
an
in-house
library
33
reference
standards,
involving
618
668
They
exhibited
a
differentiated
performance
complementarity
identifying
multiple
subclasses
components.
Chinese Herbal Medicines,
Год журнала:
2023,
Номер
16(1), С. 27 - 41
Опубликована: Ноя. 14, 2023
Traditional
Chinese
medicines
(TCMs)
possess
a
rich
historical
background,
unique
theoretical
framework,
remarkable
therapeutic
efficacy,
and
abundant
resources.
However,
the
modernization
internationalization
of
TCMs
have
faced
significant
obstacles
due
to
their
diverse
ingredients
unknown
mechanisms.
To
gain
deeper
insights
into
phytochemicals
ensure
quality
control
TCMs,
there
is
an
urgent
need
enhance
analytical
techniques.
Currently,
two-dimensional
(2D)
chromatography,
which
incorporates
two
independent
separation
mechanisms,
demonstrates
superior
capabilities
compared
traditional
one-dimensional
(1D)
system
when
analyzing
samples.
Over
past
decade,
new
techniques
been
continuously
developed
actionable
from
complex
This
review
presents
recent
advancements
in
application
multidimensional
chromatography
for
evaluation
encompassing
2D-gas
(GC),
2D-liquid
(LC),
as
well
emerging
three-dimensional
(3D)-GC,
3D-LC,
associated
data-processing
approaches.
These
studies
highlight
promising
potential
chromatographic
future
phytochemical
analysis.
Nevertheless,
increased
capability
has
resulted
higher-order
data
sets
greater
demands
tools.
Considering
that
still
relatively
nascent
research
field,
further
hardware
enhancements
implementation
chemometric
methods
are
necessary
foster
its
robust
development.
Arabian Journal of Chemistry,
Год журнала:
2023,
Номер
17(2), С. 105512 - 105512
Опубликована: Дек. 5, 2023
Compatibility
with
the
varying
prescription
ratios
is
a
unique
feature
in
Chinese
medicine
practice.
Sishen
formula
(SSF;
containing
Psoraleae
Fructus-PF,
Myristicae
Semen-MyS,
Schisandrae
Chinensis
Fructus-SCF,
and
Euodiae
Fructus-EF)
different
proportions
has
been
used
to
treat
irritable
bowel
syndrome,
however,
underlying
chemical
difference
needs
be
clarified,
particularly
influence
on
extracting
those
known
toxic
components.
To
tackle
this
issue,
an
integrated
strategy
was
developed:
1)
systematic
multicomponent
characterization
by
off-line
two-dimensional
liquid
chromatography/ion
mobility
time-of-flight
mass
spectrometry
(2D-LC/IM-QTOF-MS)
approach
HDMSE-HDDDA
hybrid
scan;
2)
discovery
of
potential
markers
associated
SSF
proportion
variation
untargeted
metabolomics;
3)
quantitative
assays
representative
multiple
reaction
monitoring
(MRM)
unveil
effects
dissolution
Consequently,
orthogonal
separation,
high
sensitivity
detection,
intelligent
data
interpretation,
enabled
233
compounds
from
decoction.
Totally
50
differential
components
were
discovered
16
high-definition
LC-MS
profiling
multivariate
statistical
analysis.
An
MRM
validated
offering
absolute
content
alternation
18
SSF.
Increasing
SCF,
MyS,
EF
moderately
could
reduce
main
coumarins
PF,
while
increasing
SCF
promoted
alkaloids
EF,
which
restrained
MyS.
Conclusively,
in-depth
achieved,
disparities
compatibility
various
exposed,
may
bridge
functions/toxicity
variations
clinic.
Journal of Separation Science,
Год журнала:
2023,
Номер
46(19)
Опубликована: Авг. 15, 2023
A
challenge
in
the
quality
control
of
traditional
Chinese
medicine
is
systematic
multicomponent
characterization
compound
formulae.
Jiawei
Fangji
Huangqi,
a
modified
form
prescription
comprising
seven
herbal
medicines.
To
address
chemical
complexity
Huangqi
decoction,
we
integrated
ion
mobility-quadrupole
time-of-flight
high-definition
MSE
coupled
to
ultra-high-performance
liquid
chromatography
and
intelligent
data
processing
workflows
available
UNIFI
software
package.
Good
chromatographic
separation
was
achieved
on
CORTECS
UPLC
T3
column
within
52
min,
high-accuracy
MS2
were
acquired
using
negative
positive
modes.
library
1250
compounds
created
incorporated
into
enable
automatic
peak
annotation
data.
We
identified
or
tentatively
characterize
430
decoction.
The
potential
superiority
over
conventional
MS
acquisition
approaches
revealed
its
spectral
(MS2
),
differentiation
isomers,
coeluting
compounds,
target
mass
coverage.
multiple
components
decoction
elucidated,
offering
insight
improved
pharmacological
action
compared
with
that
formula.
PLoS ONE,
Год журнала:
2023,
Номер
18(8), С. e0290696 - e0290696
Опубликована: Авг. 25, 2023
Ephedra
is
one
of
the
world's
most
important
plants,
used
in
medicine,
plants
and
ecology.
Most
grows
plain
areas
stable.
But
plateau
environment
special,
with
change
altitude,
variety
difference
saxatilis
very
obvious.
E.
metabolism
on
Tibetan
Plateau
not
only
affected
by
but
also
environmental
conditions
such
as
climate
different
soil
components.
However,
mechanism
alkaloids
special
ecological
still
unclear.
Therefore,
we
analyzed
metabolic
altitude
species
Plateau.
Through
functional
analysis
Kyoto
Metabolism
Metabolomic
Encyclopedia
(KEGG),
can
determine
that
number
metabolites
decreases
increase
there
are
differences
among
three
mountains.
This
was
confirmed
univariate
top
five
pathways.
Based
metabolomics,
it
found
water
content
a
factor
affecting
metabolism.
According
to
vertical
height
gradient,
ephedrine
pseudephedrine
showed
same
under
Ephedrine
increased
gradient
increased,
pseudoephedrine
decreased
decreased.
Our
results
provide
valuable
information
for
further
study
efficacy
stability.
It
provides
useful
reference
research
planting
area.
