Biomedicines,
Год журнала:
2022,
Номер
10(4), С. 784 - 784
Опубликована: Март 27, 2022
Enzymatic
nanoreactors
are
enzyme-encapsulated
nanobodies
that
capable
of
performing
biosynthetic
or
catabolic
reactions.
For
this
paper,
we
focused
on
therapeutic
enzyme
for
the
neutralization
toxicants,
paying
special
attention
to
inactivation
organophosphorus
compounds
(OP).
Therapeutic
enzymes
detoxifying
OPs
known
as
bioscavengers.
The
encapsulation
injectable
bioscavengers
by
nanoparticles
was
first
used
prevent
fast
clearance
and
immune
response
heterologous
enzymes.
aim
is
also
provide
a
high
concentration
reactive
in
stable
nanocontainers.
Under
these
conditions,
detoxification
reaction
takes
place
inside
compartment,
where
much
higher
than
toxicant
diffusing
across
nanoreactor
membrane.
Thus,
determination
encapsulated
an
important
issue
biotechnology.
implications
second-order
nanoreactor's
permeability
terms
substrates,
products
their
possible
osmotic,
viscosity,
crowding
effects
examined.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2023,
Номер
10
Опубликована: Янв. 4, 2023
Coacervates
are
droplets
formed
by
liquid‒liquid
phase
separation.
An
increasing
number
of
studies
have
reported
that
coacervates
play
an
important
role
in
living
cells,
such
as
the
generation
membraneless
organelles,
and
peptides
contribute
to
condensate
droplet
formation.
Peptides
with
versatile
functional
groups
special
secondary
structures,
including
α-helices,
β-sheets
intrinsically
disordered
regions,
provide
novel
insights
into
coacervation,
biomimetic
protocells,
neurodegenerative
diseases,
modulations
signal
transmission,
drug
delivery
systems.
In
this
review,
we
introduce
different
types
peptide-based
principles
their
interactions.
Additionally,
summarize
thermodynamic
kinetic
mechanisms
associated
factors,
salt,
pH,
temperature,
affecting
separation
process.
We
illustrate
recent
on
modulating
functions
applied
biological
diseases.
Finally,
propose
promising
broad
applications
describe
challenges
future.
Biomacromolecules,
Год журнала:
2023,
Номер
24(11), С. 4587 - 4604
Опубликована: Окт. 16, 2023
Compartmentalization
is
a
crucial
feature
of
natural
cell,
manifested
in
cell
membrane
and
inner
lumen.
Inspired
by
the
cellular
structure,
multicompartment
polymersomes
(MCPs),
including
membrane-compartmentalized
lumen-compartmentalized
(polymersomes-in-polymersomes),
have
aroused
great
expectations
for
biological
applications
such
as
biocatalysis
mimics
past
decades.
Compared
with
traditional
polymersomes,
MCPs
advantages
encapsulating
multiple
enzymes
separately
multistep
enzymatic
cascade
reactions.
In
this
review,
first,
design
principles
preparation
methods
are
summarized.
Next,
recent
advances
nanoreactors
to
mimic
subcellular
organelles
or
artificial
cells
discussed.
Finally,
future
research
directions
prospected.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(14), С. 7822 - 7822
Опубликована: Июль 17, 2024
Mining
of
organophosphorous
(OPs)-degrading
bacterial
enzymes
in
collections
known
strains
and
natural
biotopes
are
important
research
fields
that
lead
to
the
isolation
novel
OP-degrading
enzymes.
Then,
implementation
strategies
methods
protein
engineering
nanobiotechnology
allow
large-scale
production
enzymes,
displaying
improved
catalytic
properties
for
medical
uses
protection
environment.
For
applications,
enzyme
formulations
must
be
stable
bloodstream
upon
storage
not
susceptible
induce
iatrogenic
effects.
This,
particular,
includes
nanoencapsulation
bioscavengers
origin.
In
application
field
bioremediation,
these
play
a
crucial
role
environmental
cleanup
by
initiating
degradation
OPs,
such
as
pesticides,
contaminated
environments.
microbial
cell
configuration,
can
break
down
chemical
bonds
OPs
usually
convert
them
into
less
toxic
metabolites
through
biotransformation
process
or
contribute
their
complete
mineralization.
purified
state,
they
exhibit
higher
pollutant
efficiencies
ability
operate
under
different
conditions.
Thus,
this
review
provides
clear
overview
current
knowledge
about
applications
OP-reacting
It
presents
works
focusing
on
use
various
bioremediation
mitigate
pollution
medicine
alternative
therapeutic
means
against
OP
poisoning.
Precision medicine and engineering.,
Год журнала:
2024,
Номер
1(1), С. 100004 - 100004
Опубликована: Авг. 13, 2024
The
vesicle,
which
consists
of
the
cytoplasm
encapsulated
by
lipid
bilayer,
has
acted
as
an
important
mediator
intercellular
communication
a
potential
tool
for
disease
treatment
during
past
decade.
In
addition
to
ability
biological
amphiphiles
form
vesicles,
artificial
vesicles
can
also
be
formed
from
synthetic
polymers,
have
been
widely
applied
in
transport,
temporary
storage
and
chemical
reaction
chambers.
this
review,
we
summarized
progress
use
polymer
nanoreactors
biomedical
applications.
First,
various
preparation
methods,
including
hydration,
polymerization-induced
self-assembly,
solvent
exchange,
emulsion
phase
transfer,
microfluidics
flash
nanoprecipitation,
are
compared
here.
Second,
applications
nanoreactors,
such
stimuli-responsive
drug
delivery,
blood–brain
barrier
penetration,
enhanced
physiological
stability,
diseases,
highlighted
review.
addition,
existing
obstacles
future
development
directions
proposed
broader
clinical
translations.
Journal of Polymer Science,
Год журнала:
2023,
Номер
61(16), С. 1730 - 1748
Опубликована: Апрель 25, 2023
Abstract
Microgels
are
soft
particles
that
offer
excellent
colloidal
stability,
large
surface
area,
and
fluid‐like
transport
characteristics,
making
them
ideal
for
enzyme
immobilization
reaction
regulation.
In
recent
years,
the
use
of
microgels
in
processes
has
rapidly
increased
reviewed.
Here
this
review,
we
have
provided
a
comprehensive
overview
strategies
involved
microgel
synthesis,
practical
aspects
microgel–enzyme
conjugate
formation,
effects
on
stabilization
activities.
end,
discussed
important
biocatalytic
applications
enzyme.
The
aim
review
is
to
provide
detailed
understanding
enzyme–microgel
conjugates
their
potential
applications,
which
can
aid
design
new
microgels,
responsive
platforms,
biochemical
applications.
Advanced Materials Technologies,
Год журнала:
2024,
Номер
9(18)
Опубликована: Май 1, 2024
Abstract
Designing
and
assembling
artificial
cells
(ACs)
is
a
core
direction
in
bottom‐up
synthetic
biology.
Here,
the
advancements
past
3
years
engineering
ACs
with
focus
on
compartmentalization
surface
modifications
aim
for
their
integration
semi‐synthetic
tissue
are
outlined.
Compartmentalization
vesicles,
coacervates
hydrogels
discussed
encapsulated
catalysis
or
cytoskeleton
formation
including
use
of
components
mammalian
to
increase
ACs’
complexity.
Following
on,
modification
reviewed
due
its
relevance
when
into
goal.
Finally,
interaction
cellular
communication
fabrication
toward
therapeutic
opportunities
outlined,
before
short
perspective
provided.
Biomacromolecules,
Год журнала:
2023,
Номер
24(12), С. 5511 - 5538
Опубликована: Ноя. 7, 2023
Multicompartment
polymersomes
(MCPs)
refer
to
that
not
only
contain
one
single
compartment,
either
in
the
membrane
or
internal
cavity,
but
also
mimic
compartmentalized
structure
of
living
cells,
attracting
much
attention
programmed
delivery
and
biological
applications.
The
investigation
MCPs
may
promote
application
soft
nanomaterials
biomedicine.
This
Review
seeks
highlight
recent
advances
design
principles,
synthetic
strategies,
biomedical
applications
MCPs.
compartmentalization
types
including
chemical,
physical,
hybrid
are
discussed.
Subsequently,
controlled
synthesis
by
self-assembly
amphiphilic
polymers,
double
emulsification,
coprecipitation,
microfluidics
particle
assembly,
etc.
summarized.
Furthermore,
diverse
various
cargoes
cancer
therapy,
antimicrobials,
regulation
blood
glucose
levels
highlighted.
Finally,
future
perspectives
from
aspects
proposed.
International Journal of Molecular Sciences,
Год журнала:
2022,
Номер
23(10), С. 5718 - 5718
Опубликована: Май 20, 2022
Nano-
and
micrometer-sized
compartments
composed
of
synthetic
polymers
are
designed
to
mimic
spatial
temporal
divisions
found
in
nature.
Self-assembly
into
such
as
polymersomes,
giant
unilamellar
vesicles
(GUVs),
layer-by-layer
(LbL)
capsules,
capsosomes,
or
polyion
complex
(PICsomes)
allows
for
the
separation
defined
environments
from
exterior.
These
can
be
further
engineered
through
incorporation
(bio)molecules
within
lumen
membrane,
while
membrane
decorated
with
functional
moieties
produce
catalytic
structures
functions.
Nanometer-sized
used
imaging,
theranostic,
therapeutic
applications
a
more
mechanically
stable
alternative
liposomes,
encapsulation
molecules,
i.e.,
enzymes,
localize
act
vivo.
On
micrometer
scale,
biohybrid
systems
encapsulate
model
proteins
form
multicompartmentalized
combination
multiple
compartments,
reaching
closer
creation
artificial
organelles
cells.
Significant
progress
modeling
strategies
has
been
achieved
both
tailored
properties
functionalizations
novel
techniques
their
assembly.
