Tailoring Peptide Coacervates for Advanced Biotechnological Applications: Enhancing Control, Encapsulation, and Antioxidant Properties DOI Creative Commons
Daniel Boas, Muhammad Taha, Edit Y. Tshuva

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Апрель 28, 2025

The increasing interest in protein and peptide coacervates is accompanied by the development of various applications, from drug delivery to biosensor preparation. However, impact end groups charges on coacervation remains unclear. For this purpose, we designed four derivatives with varying net charges. These inherently fluorescent peptides readily formed solution or during evaporation. ability control process, coacervate's appearance, encapsulation capabilities were thoroughly investigated. displayed significant antioxidant properties, protecting encapsulated material. Additionally, deposition surfaces was achieved. abilities highlight potential these biotechnological including compounds such as drugs dietary supplements. Exploiting dynamic characteristics unique properties underscores their practical advantages.

Язык: Английский

Peptide-based coacervates in therapeutic applications DOI Creative Commons

Lilusi Ma,

Xiaocui Fang, Chen Wang

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2023, Номер 10

Опубликована: Янв. 4, 2023

Coacervates are droplets formed by liquid‒liquid phase separation. An increasing number of studies have reported that coacervates play an important role in living cells, such as the generation membraneless organelles, and peptides contribute to condensate droplet formation. Peptides with versatile functional groups special secondary structures, including α-helices, β-sheets intrinsically disordered regions, provide novel insights into coacervation, biomimetic protocells, neurodegenerative diseases, modulations signal transmission, drug delivery systems. In this review, we introduce different types peptide-based principles their interactions. Additionally, summarize thermodynamic kinetic mechanisms associated factors, salt, pH, temperature, affecting separation process. We illustrate recent on modulating functions applied biological diseases. Finally, propose promising broad applications describe challenges future.

Язык: Английский

Процитировано

17

Membrane and Lumen-Compartmentalized Polymersomes for Biocatalysis and Cell Mimics DOI
Qingmei Sun, Junqiu Shi, Hui Sun

и другие.

Biomacromolecules, Год журнала: 2023, Номер 24(11), С. 4587 - 4604

Опубликована: Окт. 16, 2023

Compartmentalization is a crucial feature of natural cell, manifested in cell membrane and inner lumen. Inspired by the cellular structure, multicompartment polymersomes (MCPs), including membrane-compartmentalized lumen-compartmentalized (polymersomes-in-polymersomes), have aroused great expectations for biological applications such as biocatalysis mimics past decades. Compared with traditional polymersomes, MCPs advantages encapsulating multiple enzymes separately multistep enzymatic cascade reactions. In this review, first, design principles preparation methods are summarized. Next, recent advances nanoreactors to mimic subcellular organelles or artificial cells discussed. Finally, future research directions prospected.

Язык: Английский

Процитировано

14

Applications of Microbial Organophosphate-Degrading Enzymes to Detoxification of Organophosphorous Compounds for Medical Countermeasures against Poisoning and Environmental Remediation DOI Open Access
Tatiana N. Pashirova, Rym Salah-Tazdaït, Djaber Tazdaït

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(14), С. 7822 - 7822

Опубликована: Июль 17, 2024

Mining of organophosphorous (OPs)-degrading bacterial enzymes in collections known strains and natural biotopes are important research fields that lead to the isolation novel OP-degrading enzymes. Then, implementation strategies methods protein engineering nanobiotechnology allow large-scale production enzymes, displaying improved catalytic properties for medical uses protection environment. For applications, enzyme formulations must be stable bloodstream upon storage not susceptible induce iatrogenic effects. This, particular, includes nanoencapsulation bioscavengers origin. In application field bioremediation, these play a crucial role environmental cleanup by initiating degradation OPs, such as pesticides, contaminated environments. microbial cell configuration, can break down chemical bonds OPs usually convert them into less toxic metabolites through biotransformation process or contribute their complete mineralization. purified state, they exhibit higher pollutant efficiencies ability operate under different conditions. Thus, this review provides clear overview current knowledge about applications OP-reacting It presents works focusing on use various bioremediation mitigate pollution medicine alternative therapeutic means against OP poisoning.

Язык: Английский

Процитировано

6

Responsive microgels and microgel assemblies in biocatalytic applications DOI Open Access
Nidhi C. Dubey, Divya Gaur, Bijay P. Tripathi

и другие.

Journal of Polymer Science, Год журнала: 2023, Номер 61(16), С. 1730 - 1748

Опубликована: Апрель 25, 2023

Abstract Microgels are soft particles that offer excellent colloidal stability, large surface area, and fluid‐like transport characteristics, making them ideal for enzyme immobilization reaction regulation. In recent years, the use of microgels in processes has rapidly increased reviewed. Here this review, we have provided a comprehensive overview strategies involved microgel synthesis, practical aspects microgel–enzyme conjugate formation, effects on stabilization activities. end, discussed important biocatalytic applications enzyme. The aim review is to provide detailed understanding enzyme–microgel conjugates their potential applications, which can aid design new microgels, responsive platforms, biochemical applications.

Язык: Английский

Процитировано

12

Polymer vesicles as nanoreactors for biomedical applications DOI Creative Commons

Zhuo Zhang,

Zhen Fan, Jianzhong Du

и другие.

Precision medicine and engineering., Год журнала: 2024, Номер 1(1), С. 100004 - 100004

Опубликована: Авг. 13, 2024

The vesicle, which consists of the cytoplasm encapsulated by lipid bilayer, has acted as an important mediator intercellular communication a potential tool for disease treatment during past decade. In addition to ability biological amphiphiles form vesicles, artificial vesicles can also be formed from synthetic polymers, have been widely applied in transport, temporary storage and chemical reaction chambers. this review, we summarized progress use polymer nanoreactors biomedical applications. First, various preparation methods, including hydration, polymerization-induced self-assembly, solvent exchange, emulsion phase transfer, microfluidics flash nanoprecipitation, are compared here. Second, applications nanoreactors, such stimuli-responsive drug delivery, blood–brain barrier penetration, enhanced physiological stability, diseases, highlighted review. addition, existing obstacles future development directions proposed broader clinical translations.

Язык: Английский

Процитировано

5

Physically and Chemically Compartmentalized Polymersomes for Programmed Delivery and Biological Applications DOI

Yaning Gao,

Chenchen Gao,

Yirong Fan

и другие.

Biomacromolecules, Год журнала: 2023, Номер 24(12), С. 5511 - 5538

Опубликована: Ноя. 7, 2023

Multicompartment polymersomes (MCPs) refer to that not only contain one single compartment, either in the membrane or internal cavity, but also mimic compartmentalized structure of living cells, attracting much attention programmed delivery and biological applications. The investigation MCPs may promote application soft nanomaterials biomedicine. This Review seeks highlight recent advances design principles, synthetic strategies, biomedical applications MCPs. compartmentalization types including chemical, physical, hybrid are discussed. Subsequently, controlled synthesis by self-assembly amphiphilic polymers, double emulsification, coprecipitation, microfluidics particle assembly, etc. summarized. Furthermore, diverse various cargoes cancer therapy, antimicrobials, regulation blood glucose levels highlighted. Finally, future perspectives from aspects proposed.

Язык: Английский

Процитировано

11

Advances in synthetic biology-based drug delivery systems for disease treatment DOI
Yujie Li, Yanan Wang,

Yin-Gen Luo

и другие.

Chinese Chemical Letters, Год журнала: 2024, Номер 35(11), С. 109576 - 109576

Опубликована: Янв. 29, 2024

Язык: Английский

Процитировано

4

From Single‐Compartment Artificial Cells to Tissue‐Like Materials DOI
Isabella N. Westensee, Paula De Dios Andres, Brigitte Städler

и другие.

Advanced Materials Technologies, Год журнала: 2024, Номер 9(18)

Опубликована: Май 1, 2024

Abstract Designing and assembling artificial cells (ACs) is a core direction in bottom‐up synthetic biology. Here, the advancements past 3 years engineering ACs with focus on compartmentalization surface modifications aim for their integration semi‐synthetic tissue are outlined. Compartmentalization vesicles, coacervates hydrogels discussed encapsulated catalysis or cytoskeleton formation including use of components mammalian to increase ACs’ complexity. Following on, modification reviewed due its relevance when into goal. Finally, interaction cellular communication fabrication toward therapeutic opportunities outlined, before short perspective provided.

Язык: Английский

Процитировано

4

Current Perspectives on Synthetic Compartments for Biomedical Applications DOI Open Access
Lukas Heuberger, Maria Korpidou, Olivia M. Eggenberger

и другие.

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(10), С. 5718 - 5718

Опубликована: Май 20, 2022

Nano- and micrometer-sized compartments composed of synthetic polymers are designed to mimic spatial temporal divisions found in nature. Self-assembly into such as polymersomes, giant unilamellar vesicles (GUVs), layer-by-layer (LbL) capsules, capsosomes, or polyion complex (PICsomes) allows for the separation defined environments from exterior. These can be further engineered through incorporation (bio)molecules within lumen membrane, while membrane decorated with functional moieties produce catalytic structures functions. Nanometer-sized used imaging, theranostic, therapeutic applications a more mechanically stable alternative liposomes, encapsulation molecules, i.e., enzymes, localize act vivo. On micrometer scale, biohybrid systems encapsulate model proteins form multicompartmentalized combination multiple compartments, reaching closer creation artificial organelles cells. Significant progress modeling strategies has been achieved both tailored properties functionalizations novel techniques their assembly.

Язык: Английский

Процитировано

15

Reversible crowdedness of pH-responsive and host–guest active polymersomes: Mimicking µm-sized cell structures DOI
Kehu Zhang, Yang Zhou, Sílvia Moreno

и другие.

Journal of Colloid and Interface Science, Год журнала: 2023, Номер 654, С. 1469 - 1482

Опубликована: Окт. 6, 2023

Язык: Английский

Процитировано

9