Exosomes secreted from cardiomyocytes suppress the sensitivity of tumor ferroptosis in ischemic heart failure DOI Creative Commons
Ye Yuan,

Zhongting Mei,

Zhezhe Qu

и другие.

Signal Transduction and Targeted Therapy, Год журнала: 2023, Номер 8(1)

Опубликована: Март 27, 2023

Abstract Heart failure (HF) patients in general have a higher risk of developing cancer. Several animal studies indicated that cardiac remodeling and HF remarkably accelerate tumor progression, highlighting cause-and-effect relationship between these two disease entities. Targeting ferroptosis, prevailing form non-apoptotic cell death, has been considered promising therapeutic strategy for human cancers. Exosomes critically contribute to proximal distant organ-organ communications play crucial roles regulating diseases paracrine manner. However, whether exosomes control the sensitivity cancer ferroptosis via cardiomyocyte-tumor crosstalk ischemic not yet explored. Here, we demonstrate myocardial infarction (MI) decreased cells canonical activator erastin or imidazole ketone mouse model xenograft tumor. Post-MI plasma potently blunted inducers both vitro Lewis lung carcinoma line LLC osteosarcoma K7M2 vivo with tumorigenesis model. The expression miR-22-3p cardiomyocytes plasma-exosomes was significantly upregulated failing hearts mice chronic MI as well. Incubation isolated from post-MI overexpression alone abrogated erastin-induced ferroptotic death vitro. Cardiomyocyte-enriched packaged transferred into cells. Inhibition cardiomyocyte-specific by AAV9 sponge increased ferroptosis. ACSL4, pro-ferroptotic gene, experimentally established target Taken together, our findings uncovered first time suppresses through releasing miR-22-3p-enriched derived cardiomyocytes. Therefore, targeting exosome-mediated cardiomyocyte/tumor pathological communication may offer novel approach ferroptosis-based antitumor therapy.

Язык: Английский

miRNAs as Biomarkers in Disease: Latest Findings Regarding Their Role in Diagnosis and Prognosis DOI Creative Commons

Carmen Elena Condrat,

Dana Claudia Thompson, Mădălina Gabriela Barbu

и другие.

Cells, Год журнала: 2020, Номер 9(2), С. 276 - 276

Опубликована: Янв. 23, 2020

MicroRNAs (miRNAs) represent a class of small, non-coding RNAs with the main roles regulating mRNA through its degradation and adjusting protein levels. In recent years, extraordinary progress has been made in terms identifying origin exact functions miRNA, focusing on their potential use both research clinical field. This review aims at improving current understanding these molecules applicability medical A thorough analysis literature consulting resources available online databases such as NCBI, PubMed, Medline, ScienceDirect, UpToDate was performed. There is promising evidence that spite lack standardized protocols regarding miRNAs practice, they constitute reliable tool for future use. These meet most required criteria being an ideal biomarker, accessibility, high specificity, sensitivity. Despite present limitations, biomarkers various conditions remain impressive As techniques evolve, we anticipate will become routine approach development personalized patient profiles, thus permitting more specific therapeutic interventions.

Язык: Английский

Процитировано

1032
MicroRNA sequence codes for small extracellular vesicle release and cellular retention DOI
Rubén García-Martín,

Guoxiao Wang,

Bruna B. Brandão

и другие.

Nature, Год журнала: 2021, Номер 601(7893), С. 446 - 451

Опубликована: Дек. 22, 2021

Язык: Английский

Процитировано

503
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets DOI Creative Commons
Qin Zhang, Dennis K. Jeppesen,

James N. Higginbotham

и другие.

Nature Cell Biology, Год журнала: 2021, Номер 23(12), С. 1240 - 1254

Опубликована: Дек. 1, 2021

Abstract Extracellular vesicles and exomere nanoparticles are under intense investigation as sources of clinically relevant cargo. Here we report the discovery a distinct extracellular nanoparticle, termed supermere. Supermeres morphologically from exomeres display markedly greater uptake in vivo compared with small exomeres. The protein RNA composition supermeres differs highly enriched cargo involved multiple cancers (glycolytic enzymes, TGFBI, miR-1246, MET, GPC1 AGO2), Alzheimer’s disease (APP) cardiovascular (ACE2, ACE PCSK9). majority is associated rather than Cancer-derived increase lactate secretion, transfer cetuximab resistance decrease hepatic lipids glycogen vivo. This study identifies functional nanoparticle replete potential circulating biomarkers therapeutic targets for host human diseases.

Язык: Английский

Процитировано

292
Roles and mechanisms of exosomal non-coding RNAs in human health and diseases DOI Creative Commons
Chen Li, Yu‐Qing Ni, Hui Xu

и другие.

Signal Transduction and Targeted Therapy, Год журнала: 2021, Номер 6(1)

Опубликована: Ноя. 10, 2021

Exosomes play a role as mediators of cell-to-cell communication, thus exhibiting pleiotropic activities to homeostasis regulation. Exosomal non-coding RNAs (ncRNAs), mainly microRNAs (miRNAs), long (lncRNAs), and circular (circRNAs), are closely related variety biological functional aspects human health. When the exosomal ncRNAs undergo tissue-specific changes due diverse internal or external disorders, they can cause tissue dysfunction, aging, diseases. In this review, we comprehensively discuss underlying regulatory mechanisms exosomes in addition, explore current knowledge on roles miRNAs, lncRNAs, circRNAs health diseases, including cancers, metabolic neurodegenerative cardiovascular autoimmune infectious determine their potential implication biomarker identification therapeutic exploration.

Язык: Английский

Процитировано

261
MicroRNA-mediated regulation of glucose and lipid metabolism DOI

Pamela Agbu,

Richard W. Carthew

Nature Reviews Molecular Cell Biology, Год журнала: 2021, Номер 22(6), С. 425 - 438

Опубликована: Март 26, 2021

Язык: Английский

Процитировано

245
A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs DOI Open Access
He Yan, Yunjie Wen,

Zimu Tian

и другие.

Nature Biomedical Engineering, Год журнала: 2023, Номер 7(12), С. 1583 - 1601

Опубликована: Апрель 27, 2023

Язык: Английский

Процитировано

169
Circulating Exosomal miR‐20b‐5p Inhibition Restores Wnt9b Signaling and Reverses Diabetes‐Associated Impaired Wound Healing DOI Creative Commons
Yuan Xiong, Lang Chen,

Chenchen Yan

и другие.

Small, Год журнала: 2019, Номер 16(3)

Опубликована: Дек. 23, 2019

Abstract At present, developing therapeutic strategies to improve wound healing in individuals with diabetes remains challenging. Exosomes represent a promising nanomaterial from which microRNAs (miRNAs) can be isolated. These miRNAs have the potential exert effects, and thus, determining contributions of specific circulating exosomes is great importance. In present study, exosomal are identified diabetic patients assessed for their roles context healing. A significant upregulation miR‐20b‐5p observed isolated type 2 mellitus (T2DM), this miRNA able suppress human umbilical vein endothelial cell angiogenesis via regulation Wnt9b/β‐catenin signaling. It found that application either or sites sufficient slow angiogenesis. mice, it knocking out significantly enhances promotes Together, these findings thus provide strong evidence highly enriched T2DM transferred cells vascular endothelium, where targets Wnt9b signaling negatively regulate functionality

Язык: Английский

Процитировано

167
Electrochemical biosensor for ultrasensitive exosomal miRNA analysis by cascade primer exchange reaction and MOF@Pt@MOF nanozyme DOI
Xinyu Li, Xinmin Li, Dandan Li

и другие.

Biosensors and Bioelectronics, Год журнала: 2020, Номер 168, С. 112554 - 112554

Опубликована: Авг. 25, 2020

Язык: Английский

Процитировано

160
Extracellular vesicles as a drug delivery system: A systematic review of preclinical studies DOI Creative Commons
Pol Escudé Martinez de Castilla, Lingjun Tong, Chenyuan Huang

и другие.

Advanced Drug Delivery Reviews, Год журнала: 2021, Номер 175, С. 113801 - 113801

Опубликована: Май 18, 2021

Язык: Английский

Процитировано

157
The crosstalk: exosomes and lipid metabolism DOI Creative Commons
Wei Wang, Neng Zhu, Tao Yan

и другие.

Cell Communication and Signaling, Год журнала: 2020, Номер 18(1)

Опубликована: Авг. 3, 2020

Abstract Exosomes have been considered as novel and potent vehicles of intercellular communication, instead “cell dust”. are consistent with anucleate cells, organelles lipid bilayer consisting the proteins abundant lipid, enhancing their “rigidity” “flexibility”. Neighboring cells or distant capable exchanging genetic metabolic information via exosomes binding to recipient cell releasing bioactive molecules, such lipids, proteins, nucleic acids. Of note, exert remarkable effects on metabolism, including synthesis, transportation degradation lipid. The disorder metabolism mediated by leads occurrence progression diseases, atherosclerosis, cancer, non-alcoholic fatty liver disease (NAFLD), obesity Alzheimer’s diseases so on. More importantly, can also affect production secretion exosomes, well interactions cells. Therefore, may be applied effective targets for diagnosis treatment diseases.

Язык: Английский

Процитировано

146