Analytical Chemistry,
Год журнала:
2022,
Номер
94(11), С. 4787 - 4793
Опубликована: Март 11, 2022
Bladder
cancer
(BC)
is
one
of
the
most
common
cancers
in
world,
with
high
morbidity
and
mortality.
It
essential
to
develop
a
non-invasive,
highly
accurate,
simple
method
for
BC
diagnosis.
This
work
proposed
fluorescent
biosensor
based
on
inorganic
nanoflares
combined
DNAzyme
walker
simultaneous
detection
exosomal
microRNAs
(miRNAs).
was
constructed
Au
nanoparticle
(AuNP)
modified
carbon
dot
(CD)-labeled
substrates
strands
(AuNP@CDs
nanoflares-DNAzyme,
APCD).
In
presence
target
miRNAs,
activated
then
cleaved
CD-labeled
automatically
walked
along
AuNP,
allowing
fluorescence
recovery.
Due
structure
functional
composition,
APCD
biosensors
demonstrated
sensitivity
specificity,
reached
limit
single
miRNA
at
femtomolar
level
wide
linear
range
from
50
fM
10
nM.
Furthermore,
analysis
BC-related
miR-133b
miR-135b
clinical
serum
specimens
achieved
consistent
qRT-PCR,
suggesting
it
potential
diagnosis
other
cancers.
The Journal of Cell Biology,
Год журнала:
2023,
Номер
222(3)
Опубликована: Фев. 16, 2023
Mammalian
genes
were
long
thought
to
be
constrained
within
somatic
cells
in
most
cell
types.
This
concept
was
challenged
recently
when
cellular
organelles
including
mitochondria
shown
move
between
mammalian
culture
via
cytoplasmic
bridges.
Recent
research
animals
indicates
transfer
of
cancer
and
during
lung
injury
vivo,
with
considerable
functional
consequences.
Since
these
pioneering
discoveries,
many
studies
have
confirmed
horizontal
mitochondrial
(HMT)
its
characteristics
consequences
been
described.
Additional
support
for
this
phenomenon
has
come
from
phylogenetic
studies.
Apparently,
trafficking
occurs
more
frequently
than
previously
contributes
diverse
processes
bioenergetic
crosstalk
homeostasis,
disease
treatment
recovery,
development
resistance
therapy.
Here
we
highlight
current
knowledge
HMT
cells,
focusing
primarily
on
vivo
systems,
contend
that
process
is
not
only
(patho)physiologically
relevant,
but
also
can
exploited
the
design
novel
therapeutic
approaches.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Март 19, 2024
Abstract
Targeting
ferroptosis,
an
iron-dependent
form
of
regulated
cell
death
triggered
by
the
lethal
overload
lipid
peroxides,
in
cancer
therapy
is
impeded
our
limited
understanding
intersection
tumour’s
metabolic
feature
and
ferroptosis
vulnerability.
In
present
study,
arginine
identified
as
a
ferroptotic
promoter
using
metabolites
library.
This
effect
mainly
achieved
through
arginine’s
conversion
to
polyamines,
which
exerts
their
potent
ferroptosis-promoting
property
H
2
O
-dependent
manner.
Notably,
expression
ornithine
decarboxylase
1
(ODC1),
critical
enzyme
catalysing
polyamine
synthesis,
significantly
activated
signal——iron
overload——through
WNT/MYC
signalling,
well
subsequent
elevated
thus
forming
ferroptosis-iron
overload-WNT/MYC-ODC1-polyamine-H
positive
feedback
loop
that
amplifies
ferroptosis.
Meanwhile,
we
notice
cells
release
enhanced
polyamine-containing
extracellular
vesicles
into
microenvironment,
thereby
further
sensitizing
neighbouring
accelerating
“spread”
tumour
region.
Besides,
supplementation
also
sensitizes
or
xenograft
tumours
radiotherapy
chemotherapy
inducing
Considering
are
often
characterized
intracellular
pools,
results
indicate
metabolism
exposes
targetable
vulnerability
represents
exciting
opportunity
for
therapeutic
strategies
cancer.
ACS Nano,
Год журнала:
2024,
Номер
18(5), С. 4269 - 4286
Опубликована: Янв. 25, 2024
The
repair
of
diabetic
wounds
remains
challenging,
primarily
due
to
the
high-glucose-derived
immune
inhibition
which
often
leads
excessive
inflammatory
response,
impaired
angiogenesis,
and
heightened
susceptibility
infection.
However,
means
reduce
immunosuppression
regulate
conversion
M2
phenotype
macrophages
under
a
high-glucose
microenvironment
using
advanced
biomaterials
for
are
not
yet
fully
understood.
Herein,
we
report
two-dimensional
carbide
(MXene)-M2
macrophage
exosome
(Exo)
nanohybrids
(FM-Exo)
promoting
wound
by
overcoming
inhibition.
FM-Exo
showed
sustained
release
macrophage-derived
exosomes
(M2-Exo)
up
7
days
exhibited
broad-spectrum
antibacterial
activity.
In
microenvironment,
relative
single
Exo,
could
significantly
induce
optimized
M2a/M2c
polarization
ratio
activating
PI3K/Akt
signaling
pathway,
proliferation,
migration
fibroblasts,
angiogenic
ability
endothelial
cells.
full-thickness
model,
effectively
regulated
status
promoted
their
transition
phenotype,
thereby
inhibiting
inflammation,
angiogenesis
through
VEGF
secretion,
improving
proper
collagen
deposition.
As
result,
healing
process
was
accelerated,
leading
better
outcome
with
reduced
scarring.
Therefore,
this
study
introduced
promising
approach
address
developing
bioactive
nanomaterials
in
environment.
Theranostics,
Год журнала:
2024,
Номер
14(5), С. 2099 - 2126
Опубликована: Янв. 1, 2024
Exosomes,
which
are
small
vesicles
enclosed
by
a
lipid
bilayer
and
released
many
cell
types,
widely
dispersed
have
garnered
increased
attention
in
the
field
of
regenerative
medicine
due
to
their
ability
serve
as
indicators
diseases
agents
with
therapeutic
potential.
Exosomes
play
crucial
role
mediating
intercellular
communication
through
transfer
biomolecules,
including
proteins,
lipids,
RNA,
other
molecular
constituents,
between
cells.
The
targeted
transport
proteins
nucleic
acids
specific
cells
has
potential
enhance
or
impair
biological
functions.
applications,
they
can
be
used
alone
combination
approaches.
examination
unique
attributes
functions
these
factors
emerged
prominent
study
realm
biomedical
research.
This
manuscript
summarizes
origins
properties
exosomes,
structural,
biological,
physical,
chemical
aspects.
paper
offers
complete
recent
progress
tissue
repair
medicine,
emphasizing
possible
implications
methods
forthcoming
regeneration
attempts.
ACS Nano,
Год журнала:
2024,
Номер
18(12), С. 8777 - 8797
Опубликована: Март 15, 2024
Stem
cell-derived
extracellular
vesicles
(EVs)
show
great
potential
for
promoting
bone
tissue
regeneration.
However,
normal
EVs
(Nor-EVs)
have
a
limited
ability
to
direct
tissue-specific
Therefore,
it
is
necessary
optimize
the
osteogenic
capacity
of
EV-based
systems
repairing
extensive
defects.
Herein,
we
that
hydrogels
loaded
with
osteoinductive
dental
pulp
stem
(Ost-EVs)
enhanced
remodeling,
resulting
in
2.23
±
0.25-fold
increase
expression
morphogenetic
protein
2
(BMP2)
compared
hydrogel
control
group.
Moreover,
Ost-EVs
led
higher
alkaline
phosphatase
(ALP)
(1.88
0.16
relative
Nor-EVs)
and
formation
orange-red
calcium
nodules
(1.38
0.10
vitro.
RNA
sequencing
revealed
showed
significantly
high
miR-1246
expression.
An
ideal
implant
should
also
adhere
surrounding
moist
tissues.
In
this
study,
were
drawn
mussel-inspired
adhesive
modification,
where
carrier
was
crafted
from
hyaluronic
acid
(HA)
polyethylene
glycol
derivatives,
showcasing
impressive
adhesion,
self-healing
capabilities,
promote
growth.
The
modified
HA
(mHA)
responsive
environmental
stimuli,
making
an
effective
delivering
EVs.
ectopic
osteogenesis
animal
model,
Ost-EV/hydrogel
system
effectively
alleviated
inflammation,
accelerated
revascularization,
promoted
mineralization.
We
further
used
rat
femoral
condyle
defect
model
evaluate
situ
Ost-EVs/hydrogel
system.
Collectively,
our
results
suggest
combined
biomaterial-based
hold
promising
treating
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Июнь 6, 2024
Abstract
Adipose-derived
stem
cells
(ADSCs)
are
a
subset
of
mesenchymal
(MSCs)
isolated
from
adipose
tissue.
They
possess
remarkable
properties,
including
multipotency,
self-renewal,
and
easy
clinical
availability.
ADSCs
also
capable
promoting
tissue
regeneration
through
the
secretion
various
cytokines,
factors,
extracellular
vesicles
(EVs).
ADSC-derived
EVs
(ADSC-EVs)
act
as
intercellular
signaling
mediators
that
encapsulate
range
biomolecules.
These
have
been
found
to
mediate
therapeutic
activities
donor
by
proliferation
migration
effector
cells,
facilitating
angiogenesis,
modulating
immunity,
performing
other
specific
functions
in
different
tissues.
Compared
themselves,
ADSC-EVs
offer
advantages
such
fewer
safety
concerns
more
convenient
transportation
storage
for
application.
As
result,
these
received
significant
attention
cell-free
agents
with
potential
future
application
regenerative
medicine.
In
this
review,
we
focus
on
recent
research
progress
regarding
medical
use
across
conditions,
wound
healing,
chronic
limb
ischemia,
myocardial
infarction,
diabetic
nephropathy,
fat
graft
survival,
bone
regeneration,
cartilage
tendinopathy
tendon
peripheral
nerve
acute
lung
injury,
among
others.
We
discuss
underlying
mechanisms
responsible
inducing
effects.
believe
deciphering
biological
effects,
associated
will
provide
foundation
developing
novel
approach
Graphical
Journal of the American Chemical Society,
Год журнала:
2024,
Номер
146(14), С. 10093 - 10102
Опубликована: Март 28, 2024
Real-time
monitoring
of
the
development
atherosclerosis
(AS)
is
key
to
management
cardiovascular
disease
(CVD).
However,
existing
laboratory
approaches
lack
sensitivity
and
specificity,
mostly
due
dearth
reliable
AS
biomarkers.
Herein,
we
developed
an
in
vivo
fluorescent
labeling
strategy
that
allows
specific
staining
foam
cell-derived
extracellular
vesicles
(EVs)
atherosclerotic
plaques,
which
are
released
into
blood
as
circulating
biomarkers
for
vitro
detection
AS.
This
relies
on
a
self-assembled
nanoprobe
could
recognize
cells
specifically,
where
probe
degraded
by
intracellular
HClO
produce
trifluoromethyl-bearing
boron-dipyrromethene
fluorophore
(termed
B-CF3),
lipophilic
dye
can
be
transferred
exosomal
membranes.
These
B-CF3-stained
EVs
detected
directly
fluorescence
spectrometer
or
microplate
reader
without
resorting
any
sophisticated
analytical
method.
liquid-biopsy
format
enables
early
real-time
differentiation
lesion
vulnerability
during
progression,
facilitating
effective
CVD
management.
ACS Nano,
Год журнала:
2024,
Номер
18(10), С. 7504 - 7520
Опубликована: Фев. 27, 2024
The
essential
role
of
the
neural
network
in
enhancing
bone
regeneration
has
often
been
overlooked
biomaterial
design,
leading
to
delayed
or
compromised
healing.
Engineered
mesenchymal
stem
cells
(MSCs)-derived
exosomes
are
becoming
increasingly
recognized
as
potent
cell-free
agents
for
manipulating
cellular
behavior
and
improving
therapeutic
effectiveness.
Herein,
MSCs
stimulated
with
nerve
growth
factor
(NGF)
regulate
exosomal
cargoes
improve
neuro-promotive
potential
facilitate
innervated
regeneration.
In
vitro
cell
experiments
showed
that
NGF-stimulated
MSCs-derived
(N-Exos)
obviously
improved
function
neurotrophic
effects
cells,
consequently,
osteogenic
osteo-reparative
cells.
Bioinformatic
analysis
by
miRNA
sequencing
pathway
enrichment
revealed
beneficial
N-Exos
may
partly
be
ascribed
NGF-elicited
multicomponent
miRNAs
subsequent
regulation
activation
MAPK
PI3K-Akt
signaling
pathways.
On
this
basis,
were
delivered
on
micropores
3D-printed
hierarchical
porous
scaffold
accomplish
sustained
release
profile
extended
bioavailability.
a
rat
model
distal
femoral
defect,
N-Exos-functionalized
significantly
induced
neurovascular
structure
formation
This
study
provided
feasible
strategy
modulate
functional
acquire
desirable
potential.
Furthermore,
developed
represent
promising
neurovascular-promotive
reparative
clinical
translation.
