World Journal of Stem Cells,
Год журнала:
2025,
Номер
17(1)
Опубликована: Янв. 17, 2025
Mesenchymal
stem
cells
(MSCs)
are
promising
candidates
for
regenerative
therapy
due
to
their
self-renewal
capability,
multilineage
differentiation
potential,
and
immunomodulatory
effects.
The
molecular
characteristics
of
MSCs
influenced
by
location.
Recently,
epidural
fat
(EF)
EF-derived
(EF-MSCs)
have
garnered
attention
potential
benefits
the
spinal
microenvironment
high
expression
neural
SC
markers.
However,
clinical
applications
limited
cell
senescence
accessibility
EF.
Although
many
studies
attempted
establish
an
immortalized,
stable
line,
immortalized
EF-MSCs
remain
be
clarified.
To
analyze
EF-MSCs.
phenotypes
were
analyzed
using
optical
microscopy.
Cell
immortalization
was
performed
lentiviral
vectors.
biomolecular
immunoblotting,
quantitative
PCR,
proteomics.
demonstrated
a
significantly
extended
lifespan
compared
control
group,
with
well-preserved
adipogenic
surface
marker
expression.
Introduction
human
telomerase
reverse
transcriptase
genes
markedly
increased
Proteomics
analysis
revealed
substantial
increase
in
DNA
replication
pathway
components
Immortalized
exhibited
enhanced
proliferative
capacity,
retained
upregulated
components.
Nature Communications,
Год журнала:
2022,
Номер
13(1)
Опубликована: Сен. 29, 2022
Abstract
Visceral
adiposity
is
a
risk
factor
for
severe
COVID-19,
and
link
between
adipose
tissue
infection
disease
progression
has
been
proposed.
Here
we
demonstrate
that
SARS-CoV-2
infects
human
undergoes
productive
in
fat
cells.
However,
susceptibility
to
the
cellular
response
depends
on
anatomical
origin
of
cells
viral
lineage.
express
more
ACE2
are
susceptible
than
their
subcutaneous
counterparts.
leads
inhibition
lipolysis
cells,
while
visceral
it
results
higher
expression
pro-inflammatory
cytokines.
Viral
load
attenuated
when
infected
with
gamma
variant.
A
similar
degree
cell
death
occurs
4-days
after
infection,
regardless
or
Hence,
replicating
altering
function
viability
depot-
lineage-dependent
fashion.
Physiological Reviews,
Год журнала:
2023,
Номер
104(2), С. 659 - 725
Опубликована: Авг. 17, 2023
Acute
myocardial
infarction
(AMI)
is
the
leading
cause
of
cardiovascular
death
and
remains
most
common
heart
failure.
Reopening
occluded
artery,
i.e.,
reperfusion,
only
way
to
save
myocardium.
However,
expected
benefits
reducing
infarct
size
are
disappointing
due
reperfusion
paradox,
which
also
induces
specific
cell
death.
These
ischemia-reperfusion
(I/R)
lesions
can
account
for
up
50%
final
size,
a
major
determinant
both
mortality
risk
failure
(morbidity).
In
this
review,
we
provide
detailed
description
inflammation
mechanisms
as
features
I/R
injury
cardioprotective
strategies
such
ischemic
postconditioning
well
their
underlying
mechanisms.
Due
biological
properties,
use
mesenchymal
stromal/stem
cells
(MSCs)
has
been
considered
potential
therapeutic
approach
in
AMI.
Despite
promising
results
evidence
safety
preclinical
studies
using
MSCs,
effects
reported
clinical
trials
not
conclusive
even
inconsistent.
discrepancies
were
attributed
many
parameters
donor
age,
vitro
culture,
storage
time
injection
window
after
AMI,
alter
MSC
properties.
context
future
directions
will
be
generate
MSCs
with
enhanced
properties
limit
tissue
thereby
reduce
improve
healing
phase
increase
postinfarct
performance.
Nature Metabolism,
Год журнала:
2023,
Номер
5(6), С. 996 - 1013
Опубликована: Июнь 19, 2023
Adipocyte
function
is
a
major
determinant
of
metabolic
disease,
warranting
investigations
regulating
mechanisms.
We
show
at
single-cell
resolution
that
progenitor
cells
from
four
human
brown
and
white
adipose
depots
separate
into
two
main
cell
fates,
an
adipogenic
structural
branch,
developing
common
progenitor.
The
gene
signature
contains
mitochondrial
activity
genes,
associates
with
genome-wide
association
study
traits
for
fat
distribution.
Based
on
extracellular
matrix
developmental
signature,
we
name
the
branch
Wnt-regulated
tissue-resident
(SWAT)
cells.
When
stripped
cells,
SWAT
display
multipotent
phenotype
by
reverting
towards
state
or
differentiating
new
dependent
media.
Label
transfer
algorithms
recapitulate
types
in
tissue
datasets.
In
conclusion,
provide
differentiation
map
adipocytes
define
cell,
providing
perspective
regulation.
Adipose
browning
has
demonstrated
therapeutic
potentials
in
several
diseases.
Here,
by
conducting
transcriptomic
profiling
at
the
single-cell
and
single-nucleus
resolution,
we
reconstituted
cellular
atlas
mouse
inguinal
subcutaneous
white
adipose
tissue
(iWAT)
thermoneutrality
or
chronic
cold
condition.
All
major
nonimmune
cells
within
iWAT,
including
stem
progenitor
(ASPCs),
mature
adipocytes,
endothelial
cells,
Schwann
smooth
muscle
were
recovered,
allowing
us
to
uncover
an
overall
detailed
blueprint
for
transcriptomes
intercellular
cross-talks
dynamics
during
brown
remodeling.
Our
findings
also
unravel
existence
of
subpopulations
ASPCs,
as
well
new
insights
on
their
interconversion
reprogramming
response
cold.
The
adipocyte
subpopulation
competent
histocompatibility
complex
class
II
(MHCII)
antigen
presentation
is
potentiated.
Furthermore,
a
subcluster
ASPC
with
CD74
expression
was
identified
precursor
this
MHCII+
adipocyte.
Beige
adipocytes
are
transdifferented
from
preexisting
lipid
generating
which
exhibit
developmental
trajectory
de
novo
differentiation
amphiregulin
(Aregs).
Two
distinct
immune-like
present
iWAT
responsive
data
reveal
fundamental
changes
cold-evoked
browning.
Cell Reports,
Год журнала:
2023,
Номер
42(4), С. 112390 - 112390
Опубликована: Апрель 1, 2023
White
adipose
tissue
(WAT)
distribution
is
sex
dependent.
Adipocyte
hyperplasia
contributes
to
WAT
in
mice
driven
by
cues
the
microenvironment,
with
females
displaying
subcutaneous
and
visceral
WAT,
while
males
ovariectomized
have
(VWAT)-specific
hyperplasia.
However,
mechanism
underlying
sex-specific
remains
elusive.
Here,
transcriptome
analysis
female
shows
that
high-fat
diet
(HFD)
induces
estrogen
signaling
adipocyte
precursor
cells
(APCs).
Analysis
of
APCs
throughout
estrous
cycle
demonstrates
increased
proliferation
only
when
proestrus
(high
estrogen)
coincides
onset
HFD
feeding.
We
further
show
receptor
α
(ERα)
required
for
this
estradiol
treatment
at
feeding
sufficient
drive
it.
This
influence
on
APC
leads
obesity
These
data
indicate
drives
ERα-dependent
obesogenic
females,
exacerbating
contributing
differential
fat
between
sexes.
