Immortalization of epidural fat-derived mesenchymal stem cells: In vitro characterization and adipocyte differentiation potential DOI
Seoung-Woo Lee, Young‐Ju Lim, Hee-Yeon Kim

et al.

World Journal of Stem Cells, Journal Year: 2025, Volume and Issue: 17(1)

Published: Jan. 17, 2025

Mesenchymal stem cells (MSCs) are promising candidates for regenerative therapy due to their self-renewal capability, multilineage differentiation potential, and immunomodulatory effects. The molecular characteristics of MSCs influenced by location. Recently, epidural fat (EF) EF-derived (EF-MSCs) have garnered attention potential benefits the spinal microenvironment high expression neural SC markers. However, clinical applications limited cell senescence accessibility EF. Although many studies attempted establish an immortalized, stable line, immortalized EF-MSCs remain be clarified. To analyze EF-MSCs. phenotypes were analyzed using optical microscopy. Cell immortalization was performed lentiviral vectors. biomolecular immunoblotting, quantitative PCR, proteomics. demonstrated a significantly extended lifespan compared control group, with well-preserved adipogenic surface marker expression. Introduction human telomerase reverse transcriptase genes markedly increased Proteomics analysis revealed substantial increase in DNA replication pathway components Immortalized exhibited enhanced proliferative capacity, retained upregulated components.

Language: Английский

Physiological and pathological roles of lipogenesis DOI
Yong Geun Jeon, Ye Young Kim, Gung Lee

et al.

Nature Metabolism, Journal Year: 2023, Volume and Issue: 5(5), P. 735 - 759

Published: May 4, 2023

Language: Английский

Citations

112
Adipose tissue at single-cell resolution DOI Creative Commons
Babukrishna Maniyadath, Qianbin Zhang, Rana K. Gupta

et al.

Cell Metabolism, Journal Year: 2023, Volume and Issue: 35(3), P. 386 - 413

Published: March 1, 2023

Language: Английский

Citations

84
Enhancing adipose tissue plasticity: progenitor cell roles in metabolic health DOI
Simon Lecoutre,

Clémentine Rebière,

Salwan Maqdasy

et al.

Nature Reviews Endocrinology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

Language: Английский

Citations

2
SARS-CoV-2 infects adipose tissue in a fat depot- and viral lineage-dependent manner DOI Creative Commons
Tatiana D. Saccon, Felippe Mousovich‐Neto, Raissa G. Ludwig

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Sept. 29, 2022

Abstract Visceral adiposity is a risk factor for severe COVID-19, and link between adipose tissue infection disease progression has been proposed. Here we demonstrate that SARS-CoV-2 infects human undergoes productive in fat cells. However, susceptibility to the cellular response depends on anatomical origin of cells viral lineage. express more ACE2 are susceptible than their subcutaneous counterparts. leads inhibition lipolysis cells, while visceral it results higher expression pro-inflammatory cytokines. Viral load attenuated when infected with gamma variant. A similar degree cell death occurs 4-days after infection, regardless or Hence, replicating altering function viability depot- lineage-dependent fashion.

Language: Английский

Citations

53
Mesenchymal stromal cells for improvement of cardiac function following acute myocardial infarction: a matter of timing DOI
Stéphanie Barrère‐Lemaire, Anne Vincent, Christian Jørgensen

et al.

Physiological Reviews, Journal Year: 2023, Volume and Issue: 104(2), P. 659 - 725

Published: Aug. 17, 2023

Acute myocardial infarction (AMI) is the leading cause of cardiovascular death and remains most common heart failure. Reopening occluded artery, i.e., reperfusion, only way to save myocardium. However, expected benefits reducing infarct size are disappointing due reperfusion paradox, which also induces specific cell death. These ischemia-reperfusion (I/R) lesions can account for up 50% final size, a major determinant both mortality risk failure (morbidity). In this review, we provide detailed description inflammation mechanisms as features I/R injury cardioprotective strategies such ischemic postconditioning well their underlying mechanisms. Due biological properties, use mesenchymal stromal/stem cells (MSCs) has been considered potential therapeutic approach in AMI. Despite promising results evidence safety preclinical studies using MSCs, effects reported clinical trials not conclusive even inconsistent. discrepancies were attributed many parameters donor age, vitro culture, storage time injection window after AMI, alter MSC properties. context future directions will be generate MSCs with enhanced properties limit tissue thereby reduce improve healing phase increase postinfarct performance.

Language: Английский

Citations

38
Adipogenic and SWAT cells separate from a common progenitor in human brown and white adipose depots DOI Creative Commons
Nagendra P. Palani,

Carla Horváth,

Pascal Timshel

et al.

Nature Metabolism, Journal Year: 2023, Volume and Issue: 5(6), P. 996 - 1013

Published: June 19, 2023

Adipocyte function is a major determinant of metabolic disease, warranting investigations regulating mechanisms. We show at single-cell resolution that progenitor cells from four human brown and white adipose depots separate into two main cell fates, an adipogenic structural branch, developing common progenitor. The gene signature contains mitochondrial activity genes, associates with genome-wide association study traits for fat distribution. Based on extracellular matrix developmental signature, we name the branch Wnt-regulated tissue-resident (SWAT) cells. When stripped cells, SWAT display multipotent phenotype by reverting towards state or differentiating new dependent media. Label transfer algorithms recapitulate types in tissue datasets. In conclusion, provide differentiation map adipocytes define cell, providing perspective regulation.

Language: Английский

Citations

29
Mitochondrial respiration is decreased in visceral but not subcutaneous adipose tissue in obese individuals with fatty liver disease DOI
Kalliopi Pafili, S. Kahl, Lucia Mastrototaro

et al.

Journal of Hepatology, Journal Year: 2022, Volume and Issue: 77(6), P. 1504 - 1514

Published: Aug. 19, 2022

Language: Английский

Citations

34
Cold-Induced Reprogramming of Subcutaneous White Adipose Tissue Assessed by Single-Cell and Single-Nucleus RNA Sequencing DOI Creative Commons
Qing Liu,

Qiaoyun Long,

Jiayu Zhao

et al.

Research, Journal Year: 2023, Volume and Issue: 6

Published: Jan. 1, 2023

Adipose browning has demonstrated therapeutic potentials in several diseases. Here, by conducting transcriptomic profiling at the single-cell and single-nucleus resolution, we reconstituted cellular atlas mouse inguinal subcutaneous white adipose tissue (iWAT) thermoneutrality or chronic cold condition. All major nonimmune cells within iWAT, including stem progenitor (ASPCs), mature adipocytes, endothelial cells, Schwann smooth muscle were recovered, allowing us to uncover an overall detailed blueprint for transcriptomes intercellular cross-talks dynamics during brown remodeling. Our findings also unravel existence of subpopulations ASPCs, as well new insights on their interconversion reprogramming response cold. The adipocyte subpopulation competent histocompatibility complex class II (MHCII) antigen presentation is potentiated. Furthermore, a subcluster ASPC with CD74 expression was identified precursor this MHCII+ adipocyte. Beige adipocytes are transdifferented from preexisting lipid generating which exhibit developmental trajectory de novo differentiation amphiregulin (Aregs). Two distinct immune-like present iWAT responsive data reveal fundamental changes cold-evoked browning.

Language: Английский

Citations

18
Fibroblast-derived CXCL14 aggravates crystalline silica–induced pulmonary fibrosis by mediating polarization and recruitment of interstitial macrophages DOI
Yichuan You, Haoyang Yuan, Min Hui

et al.

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 460, P. 132489 - 132489

Published: Sept. 8, 2023

Language: Английский

Citations

18
Estradiol cycling drives female obesogenic adipocyte hyperplasia DOI Creative Commons
Rocío del M. Saavedra-Peña, Natalia Taylor, Clare A. Flannery

et al.

Cell Reports, Journal Year: 2023, Volume and Issue: 42(4), P. 112390 - 112390

Published: April 1, 2023

White adipose tissue (WAT) distribution is sex dependent. Adipocyte hyperplasia contributes to WAT in mice driven by cues the microenvironment, with females displaying subcutaneous and visceral WAT, while males ovariectomized have (VWAT)-specific hyperplasia. However, mechanism underlying sex-specific remains elusive. Here, transcriptome analysis female shows that high-fat diet (HFD) induces estrogen signaling adipocyte precursor cells (APCs). Analysis of APCs throughout estrous cycle demonstrates increased proliferation only when proestrus (high estrogen) coincides onset HFD feeding. We further show receptor α (ERα) required for this estradiol treatment at feeding sufficient drive it. This influence on APC leads obesity These data indicate drives ERα-dependent obesogenic females, exacerbating contributing differential fat between sexes.

Language: Английский

Citations

17