Osteoarthritis and Cartilage, Год журнала: 2025, Номер unknown
Опубликована: Март 1, 2025
Язык: Английский
Science China Life Sciences, Год журнала: 2023, Номер 66(5), С. 893 - 1066
Опубликована: Апрель 11, 2023
Язык: Английский
Процитировано
197
Journal of Bone and Mineral Research, Год журнала: 2023, Номер 38(3), С. 359 - 369
Опубликована: Янв. 18, 2023
Bone remodeling in the adult skeleton facilitates removal and replacement of damaged old bone to maintain quality. Tight coordination resorption formation during crucially maintains skeletal mass. Increasing evidence suggests that many cell types beyond osteoclasts osteoblasts support remodeling, including macrophages other myeloid lineage cells. Herein, we discuss origin functions for microenvironment, tissue resident macrophages, osteomacs, as well newly identified osteomorphs result from osteoclast fission. We also touch on role inflammatory resorption. © 2023 The Authors. Journal Mineral Research published by Wiley Periodicals LLC behalf American Society (ASBMR).
Язык: Английский
Процитировано
60
Cell Metabolism, Год журнала: 2024, Номер 36(5), С. 1144 - 1163.e7
Опубликована: Апрель 3, 2024
Язык: Английский
Процитировано
17
Advanced Science, Год журнала: 2023, Номер 10(31)
Опубликована: Сен. 26, 2023
Abstract Blood vessels play a role in osteogenesis and osteoporosis; however, the of vascular metabolism these processes remains unclear. The present study finds that ovariectomized mice exhibit reduced blood vessel density bone expression endothelial glycolytic regulator pyruvate kinase M2 (PKM2). Endothelial cell (EC)‐specific deletion Pkm2 impairs worsens osteoporosis mice. This is attributed to impaired ability mesenchymal stem cells (BMSCs) differentiate into osteoblasts. Mechanistically, EC‐specific reduces serum lactate levels secreted by ECs, which affect histone lactylation BMSCs. Using joint CUT&Tag RNA sequencing analyses, collagen type I alpha 2 chain (COL1A2), cartilage oligomeric matrix protein (COMP), ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), transcription factor 7 like (TCF7L2) as osteogenic genes regulated H3K18la are identified. PKM2 overexpression addition, exercise restore phenotype PKM2‐deficient Furthermore, metabolomics indicate patients with have relatively low levels. Additionally, related BMSCs downregulated osteoporosis. In conclusion, glycolysis ECs fuels BMSC differentiation osteoblasts through lactylation, partially ameliorates increasing
Язык: Английский
Процитировано
32
Bone Research, Год журнала: 2024, Номер 12(1)
Опубликована: Авг. 5, 2024
Abstract Ageing as a natural irreversible process inherently results in the functional deterioration of numerous organ systems and tissues, including skeletal immune systems. Recent studies have elucidated intricate bidirectional interactions between these two In this review, we provide comprehensive synthesis molecular mechanisms cell ageing. We further discuss how age-related changes influence system consequent impact alterations on system. Finally, highlight clinical implications findings propose potential strategies to promote healthy ageing reduce pathologic both
Язык: Английский
Процитировано
13
Ageing Research Reviews, Год журнала: 2024, Номер 99, С. 102235 - 102235
Опубликована: Фев. 16, 2024
Язык: Английский
Процитировано
12
Signal Transduction and Targeted Therapy, Год журнала: 2024, Номер 9(1)
Опубликована: Май 29, 2024
Despite substantial evidence emphasizing the pleiotropic benefits of exercise for prevention and treatment various diseases, underlying biological mechanisms have not been fully elucidated. Several attributed to signaling molecules that are released in response by different tissues such as skeletal muscle, cardiac adipose, liver tissue. These molecules, which collectively termed exerkines, form a heterogenous group bioactive substances, mediating inter-organ crosstalk well structural functional tissue adaption. Numerous scientific endeavors focused on identifying characterizing new mediators with properties. Additionally, some investigations molecular targets exerkines cellular cascades trigger adaption processes. A detailed understanding tissue-specific downstream effects is crucial harness health-related mediated improve targeted programs health disease. Herein, we review current vivo exerkine-induced signal transduction across multiple target highlight preventive therapeutic value exerkine diseases. By aspects research, provide comprehensive overview (i) underpinnings secretion, (ii) receptor-dependent receptor-independent adaption, (iii) clinical implications these disease treatment.
Язык: Английский
Процитировано
12
Advanced Science, Год журнала: 2024, Номер 11(14)
Опубликована: Фев. 2, 2024
Abstract Chronic diabetic wounds are a serious complication of diabetes and often result in limb amputations confer high mortality rates. The proinflammatory secretome the wound perpetuates defective neovascularization contributes to dysregulated tissue repair. This study aims design gelatin methacrylamide (GelMA) hydrogel sustained release grancalcin‐neutralizing antibody (GCA‐NAb) evaluate it as potential scaffold promote healing. Results show that expression grancalcin(GCA), protein secreted by bone marrow‐derived immune cells, is elevated sites individuals animals with ulcers. Genetic inhibition grancalcin accelerates vascularization healing an animal model. Mechanistic studies binds transient receptor melastatin 8(TRPM8) partially inactivates its downstream signaling pathways, thereby impairing angiogenesis vitro ex vivo. Systemic or topical administration GCA‐NAb accelerate repair mice diabetes. data suggest GCA therapeutic target for treatment
Язык: Английский
Процитировано
10
Cell Research, Год журнала: 2025, Номер unknown
Опубликована: Янв. 29, 2025
Abstract Sleep deficiency is associated with obesity, but the mechanisms underlying this connection remain unclear. Here, we identify a sleep-inducible hypothalamic protein hormone in humans and mice that suppresses obesity. This cleaved from reticulocalbin-2 (RCN2), name it Raptin. Raptin release timed by circuit vasopressin-expressing neurons suprachiasmatic nucleus to RCN2-positive paraventricular nucleus. levels peak during sleep, which blunted sleep deficiency. binds glutamate metabotropic receptor 3 (GRM3) of hypothalamus stomach inhibit appetite gastric emptying, respectively. Raptin-GRM3 signaling mediates anorexigenic effects via PI3K-AKT signaling. Of note, verify connections between deficiencies sleeping state, impaired release, obesity patients Moreover, carrying an RCN2 nonsense variant present night eating syndrome These data define unique food intake prevents
Язык: Английский
Процитировано
1
JCI Insight, Год журнала: 2024, Номер 9(4)
Опубликована: Янв. 11, 2024
RNA-binding proteins (RBPs) interact with RNA and ubiquitously regulate transcripts during their life cycle, playing a fundamental role in the progression of angiogenesis-related diseases. In skeletal system, endothelium-dependent angiogenesis is indispensable for bone formation. However, RBPs formation unclear. Here, we show that RBP-Y-box-binding protein 1 (YBX1) was strongly reduced vasculature ovariectomy (OVX) mice. Endothelial cell-specific deletion Ybx1 impaired CD31-high, endomucin-high (CD31hiEMCNhi) endothelium morphology resulted low mass whereas overexpression promoted angiogenesis-dependent osteogenesis ameliorated loss. Mechanistically, YBX1 disrupted CD31, EMCN, morphogenetic 4 (BMP4) stability an m5C-dependent manner blocked endothelium-derived BMP4 release, thereby inhibiting osteogenic differentiation mesenchymal stromal cells. Administration recombinant restored Tail vein injection CD31-modified polyethylene glycol-poly (lactic-co-glycolic acid) carrying sciadopitysin, natural agonist, pharmacologically partially reversed CD31hiEMCNhi vessels' decline improved both OVX aging animals. These findings demonstrated RBP-YBX1 provided therapeutic approach ameliorating osteoporosis.
Язык: Английский
Процитировано
6