Comprehensive bioinformatics analysis of metabolism‑related microRNAs in high myopia in young and old adults with age‑related cataracts DOI Creative Commons
Fanfan Huang, Yanyi Chen,

Jiaxue Wu

и другие.

Molecular Medicine Reports, Год журнала: 2024, Номер 31(2)

Опубликована: Дек. 3, 2024

High myopia and age‑related cataracts are prevalent ocular disorders that compromise visual acuity. The molecular mechanisms underlying these conditions remain largely unclear. Here, microRNA (miRNA or miR) sequencing was performed on aqueous humor samples obtained from individuals with high (AH, n=9), young patients (YH, n=9) a control group of elderly cataracts, matched in terms sex age (AN, n=9). miRNA differential expression were performed. Intersecting miRNAs identified, as well metabolism‑related genes MsigDB intersected target genes. Functional enrichment disease targets predicted using DisGeNET. A protein‑protein interaction network built STRING, hub identified via Cytoscape. GeneMANIA analyzed genes, while drug predictions made Comparative Toxicogenomics Database. Long non‑coding RNAs transcription factors mirNet ChEA3. Results validated by RT‑qPCR. total 18 significantly expressed between AH AN group, which eight up‑ 10 downregulated. 23 the YH six 17 hsa‑miR‑490‑3p, hsa‑miR‑4423‑3p hsa‑miR‑4485‑3p may serve characteristic miRNAs. 289 predicted. analysis yielded 169 terms, 'herpes simplex virus 1 infection' most enriched. There 19 metabolism‑associated linked miRNAs, suggesting potential role metabolic processes pathogenesis conditions. biosynthetic process carbohydrate derivatives key during development myopia. Propionyl‑CoA Carboxylase Subunit β could potentially biomarker. Drugs modulate their function predicted; cyclosporine, tretinoin acetaminophen exert broad influence Hub gene networks based constructed to predict 44 associated long 98 factors. present findings offer novel insights into propose therapeutic targets.

Язык: Английский

Expression of SLC2A1 is upregulated in a mouse model of age-related hearing loss DOI
Jiali Liu,

Haisen Peng,

Yuehui Liu

и другие.

Cellular Signalling, Год журнала: 2025, Номер 131, С. 111776 - 111776

Опубликована: Март 30, 2025

Язык: Английский

Процитировано

0
Inhibition of lens-induced myopia in guinea pigs using a far-induced infrared ray material DOI Creative Commons

Weiwei Xu,

Jingxi Liu,

Guoji Erti

и другие.

Frontiers in Medicine, Год журнала: 2025, Номер 12

Опубликована: Март 31, 2025

Numerous studies have demonstrated a close relationship between choroidal thickness (ChT), sclera/choroidal hypoxia, and the onset progression of myopia. Far-infrared (FIR) therapy is traditional method used to enhance microcirculation. In this study, we estimated effectiveness FIR in myopia control explored its underlying mechanisms. Furthermore, compared efficacy from two different sources controlling Guinea pigs were divided into three groups, all which underwent minus lens induction for 4 weeks. Two groups received simultaneous intervention, either radiator (FIRR) lamp or an innovative material (FIRM). Refraction, axial length (AL), ChT, levels hypoxia-labeled pimonidazole choroid sclera measured. Both FIRR FIRM inhibited increases refraction AL attenuate decrease ChT. They also mitigated scleral hypoxia. Compared FIRR, greater effect on hypoxia attenuation. However, difference reduction was not statistically significant. effectively controls myopia, may represent breakthrough management near future.

Язык: Английский

Процитировано

0
The emerging role of protein l-lactylation in metabolic regulation and cell signalling DOI

Haowen Ren,

Yuwei Tang, Di Zhang

и другие.

Nature Metabolism, Год журнала: 2025, Номер unknown

Опубликована: Апрель 2, 2025

Язык: Английский

Процитировано

0
Emerging roles of lysine lactyltransferases and lactylation DOI
Zhi Zong,

Jiang Ren,

Bing Yang

и другие.

Nature Cell Biology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 4, 2025

Язык: Английский

Процитировано

0
Histone lactylation-augmented IRF4 is implicated in arsenite-induced liver fibrosis via modulating Th17 cell differentiation DOI

Weiyong Chen,

Peiwen Wang,

Yan Xie

и другие.

Chemico-Biological Interactions, Год журнала: 2025, Номер unknown, С. 111507 - 111507

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Metabolic reprogramming in skeletal cell differentiation DOI Creative Commons

J. Bertels,

Guangxu He, Fanxin Long

и другие.

Bone Research, Год журнала: 2024, Номер 12(1)

Опубликована: Окт. 11, 2024

Abstract The human skeleton is a multifunctional organ made up of multiple cell types working in concert to maintain bone and mineral homeostasis perform critical mechanical endocrine functions. From the beginning steps chondrogenesis that prefigures most skeleton, rapid accrual during skeletal growth, followed by remodeling mature differentiation integral health. While growth factors nuclear proteins influence have been extensively studied, role cellular metabolism just be uncovered. Besides energy production, metabolic pathways shown exert epigenetic regulation via key metabolites fate both cancerous normal tissues. In this review, we will assess transcription reprogramming meet energetic biosynthetic needs chondrocytes, osteoblasts, or osteoclasts. We also summarize emerging evidence linking changes modifications differentiation.

Язык: Английский

Процитировано

3
Lactate-mediated lactylation in human health and diseases: Progress and remaining challenges DOI Creative Commons

Xueting Hu,

Xiaofeng Wu, Jinyi Xu

и другие.

Journal of Advanced Research, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

Lactate was once considered as metabolic waste for a long time. In 2019, Professor Zhao Yingming's team from the University of Chicago found that lactate could also be used substrate to induce histone lactylation and regulate gene expression. Since then, researchers have discovered lactate-mediated play important regulatory roles in various physiological pathological processes.

Язык: Английский

Процитировано

3
Animal modeling for myopia DOI Creative Commons

Lingman Zheng,

Zhiyong Liao, Jian Zou

и другие.

Advances in Ophthalmology Practice and Research, Год журнала: 2024, Номер 4(4), С. 173 - 181

Опубликована: Июнь 5, 2024

Myopia is one of the most common eye diseases globally, and has become an increasingly serious health concern among adolescents. Understanding factors contributing to onset myopia strategies slow its progression critical reducing prevalence.

Язык: Английский

Процитировано

2
No Evidence of An Association between Genetic Factors Affecting Response to Vitamin A Supplementation and Myopia: A Mendelian Randomization Study and Meta-Analysis DOI Open Access
Xiaotong Xu, N N Liu,

Weihong Yu

и другие.

Nutrients, Год журнала: 2024, Номер 16(12), С. 1933 - 1933

Опубликована: Июнь 18, 2024

The relationship between vitamin A supplementation and myopia has been a topic of debate, with conflicting inconclusive findings. We aimed to determine whether there is causal the risk using Mendelian randomization (MR) meta-analytical methods. Genetic variants from UK Biobank FinnGen studies associated response were employed as instrumental variables evaluate myopia. Fixed-effects meta-analysis was then used combine MR estimates multiple sources for each outcome. results found no convincing evidence support direct (odds ratio (OR) = 0.99, 95% confidence interval (CI) 0.82–1.20, I2 0%, p 0.40). analysis three out four sets analyses indicated direction effect, whereas other set suggested that higher lower (OR 0.002, CI 1.17 × 10−6–3.099, 0.096). This comprehensive study did not find valid association Vitamin may have an independent effect on myopia, but intraocular processes indirectly contribute its development.

Язык: Английский

Процитировано

2
Histone H4K12 lactylation promotes malignancy progression in triple-negative breast cancer through SLFN5 downregulation DOI Creative Commons
Jingyi Li, Ziyu Chen, Mingming Jin

и другие.

Cellular Signalling, Год журнала: 2024, Номер 124, С. 111468 - 111468

Опубликована: Окт. 11, 2024

Lactylation, a newly identified post-translational modification, is uncertain in its implication triple-negative breast cancer (TNBC). In this study, we analyzed 60 TNBC samples using immunohistochemical staining and revealed elevated levels of pan-lactylated proteins specific histone H4K12 lactylation tumor tissues, correlating with progression. Lactate exposure cell lines significantly induced lysine at the site, leading to alterations gene profiles reduced apoptosis. These effects were attenuated by DCA or sodium Oxamate, inhibitors endogenous lactate production. Gene sequencing showed an increase Schlafen 5 (SLFN5) expression cells treated contrasting exposure. Analysis tissues negative correlation between SLFN5 protein levels. Overexpression countered on apoptosis growth, highlighting pivotal role malignancy. CUT&Tag indicated that lactylated potentially binds promoter region. Luciferase reporter assays further verified lactate-induced suppression activity mediated wild-type H4K12, but not R A mutants, both vitro vivo detection response Oxamate stimulation. results establish lactylation, cells, specifically suppresses expression, contributing Our findings illuminate critical lactylation-dependent carcinogenic pathway TNBC.

Язык: Английский

Процитировано

2