Biosensors for rapid detection of bacterial pathogens in water, food and environment

Environment International, Год журнала: 2022, Номер 166, С. 107357 - 107357

Опубликована: Июнь 18, 2022

Conventional techniques (e.g., culture-based method) for bacterial detection typically require a central laboratory and well-trained technicians, which may take several hours or days. However, recent developments within various disciplines of science engineering have led to major paradigm shift in how microorganisms can be detected. The analytical sensors are widely used medical applications the literature being extended rapid on-site monitoring pathogens food, water environment. Especially, low-resource settings such as low middle-income countries, due advantages cost, rapidness potential field-testing, their use is indispensable sustainable development regions. Within this context, paper discusses methods biosensors ensure food safety, quality environmental monitoring. In brief, most our discussion focused on including microfluidic chips. performances sensitivity, specificity usability these sensors, well brief comparison with conventional bacteria detection, form core part discussion. Furthermore, we provide holistic viewpoint future research should focus exploring synergy different sensing technologies by developing an integrated multiplexed, sensitive accurate that will enable

Язык: Английский

---

Lateral flow assays for viruses diagnosis: Up-to-date technology and future prospects

TrAC Trends in Analytical Chemistry, Год журнала: 2022, Номер 157, С. 116725 - 116725

Опубликована: Июль 5, 2022

Язык: Английский

---

Advanced Molecular and Immunological Diagnostic Methods to Detect SARS-CoV-2 Infection

Microorganisms, Год журнала: 2022, Номер 10(6), С. 1193 - 1193

Опубликована: Июнь 10, 2022

COVID-19 emerged in late 2019 China and quickly spread across the globe, causing over 521 million cases of infection 6.26 deaths to date. After 2 years, numerous advances have been made. First all, preventive vaccine, which has implemented record time, is effective more than 95% cases. Additionally, diagnostic field, there are molecular antigenic kits that equipped with high sensitivity specificity. Real Time-PCR-based assays for detection viral RNA currently considered gold-standard method SARS-CoV-2 diagnosis can be used efficiently on pooled nasopharyngeal, or oropharyngeal samples widespread screening. Moreover, additional, advanced methods such as droplet-digital PCR (ddPCR), clustered regularly interspaced short palindromic repeats (CRISPR) next-generation sequencing (NGS), under development detect RNA. However, number subjects infected continuously increases globally, health care systems being placed increased stress. Thus, clinical laboratory plays an important role, helping select especially asymptomatic individuals who actively carrying live replicating virus, fast non-invasive technologies. Recent strategies, other methods, adopted either antigens, i.e., antigen-based immunoassays, human anti-SARS-CoV-2 antibodies, antibody-based nasal swabs, well blood saliva samples. role mucosal sIgAs, essential control viruses entering body through surfaces, remains elucidated, particular immune response counteracting infection, primarily at site(s) virus entry appears promising.

Язык: Английский

---

SARS‐CoV‐2 spike mRNA vaccine sequences circulate in blood up to 28 days after COVID‐19 vaccination

Apmis, Год журнала: 2023, Номер 131(3), С. 128 - 132

Опубликована: Янв. 17, 2023

In Denmark, vaccination against Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) has been with the Pfizer-BioNTech (BTN162b2) or Moderna (mRNA-1273) mRNA vaccines. Patients chronic hepatitis C virus (HCV) infection followed in our clinic received vaccinations according to Danish roll-out plan. To monitor HCV infection, RNA was extracted from patient plasma and sequencing performed on Illumina platform. 10 of 108 samples, full-length traces SARS-CoV-2 spike vaccine sequences were found blood up 28 days after COVID-19 vaccination. Detection adds important knowledge regarding this technology should lead further research into design lipid-nanoparticles half-life these vaccines humans.

Язык: Английский

---

Insufficient response to mRNA SARS-CoV-2 vaccine and high incidence of severe COVID-19 in kidney transplant recipients during pandemic

American Journal of Transplantation, Год журнала: 2021, Номер 22(3), С. 801 - 812

Опубликована: Дек. 3, 2021

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mRNA vaccination may fail to sufficiently protect transplant recipients against disease 2019 (COVID-19). We retrospectively evaluated COVID-19 in kidney (n = 226) after BNT162b2 vaccine administration. The control group consisted of unvaccinated patients 194) during the previous pandemic wave. measured anti-spike protein immunoglobulin G (IgG) levels and cellular responses, using enzyme-linked immunosorbent spot assay, a prospective cohort 31) recovery from 19). was diagnosed 37 (16%) vaccinated 43 (22%) patients. severity similar both groups, with exhibiting comparable need for hospitalization (41% vs. 40%, p 1.000) mortality (14% 9%, .726). Short posttransplant periods were associated (p < .001). Only 5 achieved positive SARS-CoV-2 IgG vaccination, 17 (89%, .001) recovered (median levels, 0.6 52.5 AU/ml, A response following present majority 22, 71%), an increase interleukin secreting T cells Despite detectable cell immunity remained at high risk severe COVID-19. Humoral responses induced by significantly lower than that

Язык: Английский

---

The use of nanotechnology in the fight against viruses: A critical review

Coordination Chemistry Reviews, Год журнала: 2022, Номер 464, С. 214559 - 214559

Опубликована: Апрель 13, 2022

Язык: Английский

---

Severe Acute Respiratory Syndrome Coronavirus‐2

Опубликована: Ноя. 22, 2024

Язык: Английский

---

Update of Guidelines for Laboratory Diagnosis of COVID-19 in Korea

Annals of Laboratory Medicine, Год журнала: 2022, Номер 42(4), С. 391 - 397

Опубликована: Фев. 17, 2022

Korean Society for Laboratory Medicine and the Korea Disease Prevention Control Agency have announced guidelines diagnosing coronavirus disease (COVID-19) in clinical laboratories Korea. With ongoing pandemic, we propose an update of previous based on new scientific data. This includes recommendations tests that were not included guidelines, including rapid molecular test, antigen antibody self-collected specimens, a revision recommendations. will aid performing laboratory COVID-19.

Язык: Английский

---

Biotechnological Perspectives to Combat the COVID-19 Pandemic: Precise Diagnostics and Inevitable Vaccine Paradigms

Cells, Год журнала: 2022, Номер 11(7), С. 1182 - 1182

Опубликована: Март 31, 2022

The outbreak of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is cause for ongoing global public health emergency. It more commonly known as disease 2019 (COVID-19); pandemic threat continues to spread aroundthe world with fluctuating emergence its new variants. severity COVID-19 ranges from asymptomatic serious distress (ARDS), which has led a high human mortality rate and disruption socioeconomic well-being. For restoration pre-pandemic normalcy, international scientific community been conducting research on war footing limit extremely pathogenic through diagnosis, treatment, immunization. Since first report viral infection, an array laboratory-based point-of-care (POC) approaches have emerged diagnosing understanding status outbreak. RT-PCR-based nucleic acid test (NAT) one rapidly developed most used detection approaches. Notably, current forbidding requires development safe, targeted vaccines/vaccine injections (shots) that can reduce associated morbidity mortality. Massive accelerated vaccination campaigns would be effective ultimate hope end pandemic. SARS-CoV-2 virus outbreak, emerging biotechnologies their multidisciplinary molecular details well wide range diagnostics potential vaccine candidates, are indispensable combating highly contagious COVID-19. Several candidates completed phase III clinical studies reported in immunizing against after rollout via emergency use authorization (EUA). However, optimizing type route delivery works best control crucial face threatening variants expected emerge over time. In conclusion, insights this review facilitate likely ideal vaccines

Язык: Английский

---

An update on lateral flow immunoassay for the rapid detection of SARS-CoV-2 antibodies

AIMS Microbiology, Год журнала: 2023, Номер 9(2), С. 375 - 401

Опубликована: Янв. 1, 2023

Over the last three years, after outbreak of COVID-19 pandemic, an unprecedented number novel diagnostic tests have been developed. Assays to evaluate immune response SARS-CoV-2 widely considered as part control strategy. The lateral flow immunoassay (LFIA), detect both IgM and IgG against SARS-CoV-2, has studied a point-of-care (POC) test. Compared laboratory tests, LFIAs are faster, cheaper user-friendly, thus available also in areas with low economic resources. Soon onset numerous kits for rapid antibody detection were put on market emergency use authorization. However, since then, scientists tried better define accuracy these their usefulness different contexts. In fact, while during first phase pandemic auxiliary molecular diagnosis COVID-19, successively became tool seroprevalence surveillance address infection policies. When 2021 massive vaccination campaign was implemented worldwide, interest LFIA reemerged due need establish extent longevity immunization vaccinated population priorities guide health policies low-income countries limited access vaccines. Here, we summarize accuracy, advantages limits POC detection, highlighting efforts that made improve this technology over few years.

Язык: Английский

---