The
COVID-19
pandemic
over
the
past
years
has
shown
a
great
need
for
rapid,
low-cost
and
on-site
detection
of
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2).
In
this
paper,
an
aptamer-based
colloidal
gold
nanoparticle
lateral
flow
test
strip
was
well
developed
to
realize
visual
wild
type
SARS-CoV-2
spike
protein
(SP)
multiple
variants.
Under
optimal
reaction
conditions,
low
limit
S
0.68
nM
acquired,
actual
recovery
83.3%
108.8%
real
world
samples.
This
suggests
potential
tool
prompt
with
good
sensitivity
accuracy,
new
method
development
alternative
antibody
strips
other
viral
targets.
Environment International,
Journal Year:
2022,
Volume and Issue:
166, P. 107357 - 107357
Published: June 18, 2022
Conventional
techniques
(e.g.,
culture-based
method)
for
bacterial
detection
typically
require
a
central
laboratory
and
well-trained
technicians,
which
may
take
several
hours
or
days.
However,
recent
developments
within
various
disciplines
of
science
engineering
have
led
to
major
paradigm
shift
in
how
microorganisms
can
be
detected.
The
analytical
sensors
are
widely
used
medical
applications
the
literature
being
extended
rapid
on-site
monitoring
pathogens
food,
water
environment.
Especially,
low-resource
settings
such
as
low
middle-income
countries,
due
advantages
cost,
rapidness
potential
field-testing,
their
use
is
indispensable
sustainable
development
regions.
Within
this
context,
paper
discusses
methods
biosensors
ensure
food
safety,
quality
environmental
monitoring.
In
brief,
most
our
discussion
focused
on
including
microfluidic
chips.
performances
sensitivity,
specificity
usability
these
sensors,
well
brief
comparison
with
conventional
bacteria
detection,
form
core
part
discussion.
Furthermore,
we
provide
holistic
viewpoint
future
research
should
focus
exploring
synergy
different
sensing
technologies
by
developing
an
integrated
multiplexed,
sensitive
accurate
that
will
enable
Microorganisms,
Journal Year:
2022,
Volume and Issue:
10(6), P. 1193 - 1193
Published: June 10, 2022
COVID-19
emerged
in
late
2019
China
and
quickly
spread
across
the
globe,
causing
over
521
million
cases
of
infection
6.26
deaths
to
date.
After
2
years,
numerous
advances
have
been
made.
First
all,
preventive
vaccine,
which
has
implemented
record
time,
is
effective
more
than
95%
cases.
Additionally,
diagnostic
field,
there
are
molecular
antigenic
kits
that
equipped
with
high
sensitivity
specificity.
Real
Time-PCR-based
assays
for
detection
viral
RNA
currently
considered
gold-standard
method
SARS-CoV-2
diagnosis
can
be
used
efficiently
on
pooled
nasopharyngeal,
or
oropharyngeal
samples
widespread
screening.
Moreover,
additional,
advanced
methods
such
as
droplet-digital
PCR
(ddPCR),
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)
next-generation
sequencing
(NGS),
under
development
detect
RNA.
However,
number
subjects
infected
continuously
increases
globally,
health
care
systems
being
placed
increased
stress.
Thus,
clinical
laboratory
plays
an
important
role,
helping
select
especially
asymptomatic
individuals
who
actively
carrying
live
replicating
virus,
fast
non-invasive
technologies.
Recent
strategies,
other
methods,
adopted
either
antigens,
i.e.,
antigen-based
immunoassays,
human
anti-SARS-CoV-2
antibodies,
antibody-based
nasal
swabs,
well
blood
saliva
samples.
role
mucosal
sIgAs,
essential
control
viruses
entering
body
through
surfaces,
remains
elucidated,
particular
immune
response
counteracting
infection,
primarily
at
site(s)
virus
entry
appears
promising.
Apmis,
Journal Year:
2023,
Volume and Issue:
131(3), P. 128 - 132
Published: Jan. 17, 2023
In
Denmark,
vaccination
against
Severe
Acute
Respiratory
Syndrome
Corona
Virus
2
(SARS-CoV-2)
has
been
with
the
Pfizer-BioNTech
(BTN162b2)
or
Moderna
(mRNA-1273)
mRNA
vaccines.
Patients
chronic
hepatitis
C
virus
(HCV)
infection
followed
in
our
clinic
received
vaccinations
according
to
Danish
roll-out
plan.
To
monitor
HCV
infection,
RNA
was
extracted
from
patient
plasma
and
sequencing
performed
on
Illumina
platform.
10
of
108
samples,
full-length
traces
SARS-CoV-2
spike
vaccine
sequences
were
found
blood
up
28
days
after
COVID-19
vaccination.
Detection
adds
important
knowledge
regarding
this
technology
should
lead
further
research
into
design
lipid-nanoparticles
half-life
these
vaccines
humans.
American Journal of Transplantation,
Journal Year:
2021,
Volume and Issue:
22(3), P. 801 - 812
Published: Dec. 3, 2021
Severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
mRNA
vaccination
may
fail
to
sufficiently
protect
transplant
recipients
against
disease
2019
(COVID-19).
We
retrospectively
evaluated
COVID-19
in
kidney
(n
=
226)
after
BNT162b2
vaccine
administration.
The
control
group
consisted
of
unvaccinated
patients
194)
during
the
previous
pandemic
wave.
measured
anti-spike
protein
immunoglobulin
G
(IgG)
levels
and
cellular
responses,
using
enzyme-linked
immunosorbent
spot
assay,
a
prospective
cohort
31)
recovery
from
19).
was
diagnosed
37
(16%)
vaccinated
43
(22%)
patients.
severity
similar
both
groups,
with
exhibiting
comparable
need
for
hospitalization
(41%
vs.
40%,
p
1.000)
mortality
(14%
9%,
.726).
Short
posttransplant
periods
were
associated
(p
<
.001).
Only
5
achieved
positive
SARS-CoV-2
IgG
vaccination,
17
(89%,
.001)
recovered
(median
levels,
0.6
52.5
AU/ml,
A
response
following
present
majority
22,
71%),
an
increase
interleukin
secreting
T
cells
Despite
detectable
cell
immunity
remained
at
high
risk
severe
COVID-19.
Humoral
responses
induced
by
significantly
lower
than
that
Annals of Laboratory Medicine,
Journal Year:
2022,
Volume and Issue:
42(4), P. 391 - 397
Published: Feb. 17, 2022
Korean
Society
for
Laboratory
Medicine
and
the
Korea
Disease
Prevention
Control
Agency
have
announced
guidelines
diagnosing
coronavirus
disease
(COVID-19)
in
clinical
laboratories
Korea.
With
ongoing
pandemic,
we
propose
an
update
of
previous
based
on
new
scientific
data.
This
includes
recommendations
tests
that
were
not
included
guidelines,
including
rapid
molecular
test,
antigen
antibody
self-collected
specimens,
a
revision
recommendations.
will
aid
performing
laboratory
COVID-19.
Cells,
Journal Year:
2022,
Volume and Issue:
11(7), P. 1182 - 1182
Published: March 31, 2022
The
outbreak
of
the
novel
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
is
cause
for
ongoing
global
public
health
emergency.
It
more
commonly
known
as
disease
2019
(COVID-19);
pandemic
threat
continues
to
spread
aroundthe
world
with
fluctuating
emergence
its
new
variants.
severity
COVID-19
ranges
from
asymptomatic
serious
distress
(ARDS),
which
has
led
a
high
human
mortality
rate
and
disruption
socioeconomic
well-being.
For
restoration
pre-pandemic
normalcy,
international
scientific
community
been
conducting
research
on
war
footing
limit
extremely
pathogenic
through
diagnosis,
treatment,
immunization.
Since
first
report
viral
infection,
an
array
laboratory-based
point-of-care
(POC)
approaches
have
emerged
diagnosing
understanding
status
outbreak.
RT-PCR-based
nucleic
acid
test
(NAT)
one
rapidly
developed
most
used
detection
approaches.
Notably,
current
forbidding
requires
development
safe,
targeted
vaccines/vaccine
injections
(shots)
that
can
reduce
associated
morbidity
mortality.
Massive
accelerated
vaccination
campaigns
would
be
effective
ultimate
hope
end
pandemic.
SARS-CoV-2
virus
outbreak,
emerging
biotechnologies
their
multidisciplinary
molecular
details
well
wide
range
diagnostics
potential
vaccine
candidates,
are
indispensable
combating
highly
contagious
COVID-19.
Several
candidates
completed
phase
III
clinical
studies
reported
in
immunizing
against
after
rollout
via
emergency
use
authorization
(EUA).
However,
optimizing
type
route
delivery
works
best
control
crucial
face
threatening
variants
expected
emerge
over
time.
In
conclusion,
insights
this
review
facilitate
likely
ideal
vaccines
