SARS-CoV-2 antibody and T cell responses one year after COVID-19 and the booster effect of vaccination: A prospective cohort study

Journal of Infection, Год журнала: 2021, Номер 84(2), С. 171 - 178

Опубликована: Дек. 9, 2021

Язык: Английский

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Regenerable and high-throughput surface plasmon resonance assay for rapid screening of anti-SARS-CoV-2 antibody in serum samples

Analytica Chimica Acta, Год журнала: 2022, Номер 1208, С. 339830 - 339830

Опубликована: Апрель 13, 2022

Язык: Английский

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Serological prevalence of SARS-CoV-2 antibody among children and young age group (between 2 and 17 years) in India: An interim result from a large multicentric population-based seroepidemiological study

Journal of Family Medicine and Primary Care, Год журнала: 2022, Номер 11(6), С. 2816 - 2823

Опубликована: Июнь 1, 2022

Estimating seroepidemiolgical prevalence of SARS-CoV-2 antibody is an essential public health strategy. There insufficient evidence among those belonging to young age population in India.To compare the seropositivity rate between children and adults selected sites from India.This was a multicentric population-based seroepidemiological study conducted urban rural areas five four states (Delhi, Odisha, Uttar Pradesh, Tripura) India. Participants aged ≥1 year were included different clusters each area. Total serum against virus assessed qualitatively by using standard enzyme-linked immunosorbent assay (ELISA) kit.Data collection period 15 March 2021 10 June 2021. available data 4509 participants, whom 700 <18 years 3809 ≥18 age. The site-wise number 2-17 92, 189, 165, 146 108 for Delhi urban, rural, Bhubaneswar Gorakhpur Agartala area, respectively. seroprevalence 55.7% group 63.5% group. female 58% male 53%.SARS-CoV-2 high comparable that adult population. Hence, it unlikely any future third wave prevailing variant would disproportionately infect 2 or older.

Язык: Английский

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COVID-19 diagnostics: Molecular biology to nanomaterials

Clinica Chimica Acta, Год журнала: 2022, Номер 538, С. 139 - 156

Опубликована: Ноя. 18, 2022

Язык: Английский

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From Detection to Protection: Antibodies and Their Crucial Role in Diagnosing and Combatting SARS-CoV-2

Vaccines, Год журнала: 2024, Номер 12(5), С. 459 - 459

Опубликована: Апрель 25, 2024

Understanding the antibody response to SARS-CoV-2, virus responsible for COVID-19, is crucial comprehending disease progression and significance of vaccine therapeutic development. The emergence highly contagious variants poses a significant challenge humoral immunity, underscoring necessity grasping intricacies specific antibodies. This review emphasizes pivotal role antibodies in shaping immune responses their implications diagnosing, preventing, treating SARS-CoV-2 infection. It delves into kinetics characteristics explores current antibody-based diagnostics, discussing strengths, clinical utility, limitations. Furthermore, we underscore potential SARS-CoV-2-specific antibodies, various therapies such as monoclonal polyclonal anti-cytokines, convalescent plasma, hyperimmunoglobulin-based therapies. Moreover, offer insights vaccines, emphasizing neutralizing order confer immunity along with emerging concern (VOCs) circulating Omicron subvariants. We also highlight challenges field, risks antibody-dependent enhancement (ADE) shed light on associated original antigenic sin (OAS) effect long COVID. Overall, this intends provide valuable insights, which are advancing sensitive diagnostic tools, identifying efficient therapeutics, developing effective vaccines combat evolving threat global scale.

Язык: Английский

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A Paper-Based Multiplexed Serological Test to Monitor Immunity against SARS-COV-2 Using Machine Learning

ACS Nano, Год журнала: 2024, Номер 18(26), С. 16819 - 16831

Опубликована: Июнь 18, 2024

The rapid spread of SARS-CoV-2 caused the COVID-19 pandemic and accelerated vaccine development to prevent virus control disease. Given sustained high infectivity evolution SARS-CoV-2, there is an ongoing interest in developing serology tests monitor population-level immunity. To address this critical need, we designed a paper-based multiplexed vertical flow assay (xVFA) using five structural proteins detecting IgG IgM antibodies changes immunity levels. Our platform not only tracked longitudinal levels but also categorized into three groups: protected, unprotected, infected, based on antibodies. We operated two xVFAs parallel detect total 40 μL human serum sample <20 min per test. After assay, images sensor panel were captured mobile phone-based custom-designed optical reader then processed by neural network-based serodiagnostic algorithm. algorithm was trained with 120 measurements/tests 30 samples from 7 randomly selected individuals blindly tested 31 8 different individuals, collected before vaccination as well after or infection, achieving accuracy 89.5%. competitive performance xVFA, along its portability, cost-effectiveness, operation, makes it promising computational point-of-care (POC) test for monitoring immunity, aiding timely decisions administration booster vaccines general public health policies protect vulnerable populations.

Язык: Английский

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Immunogenicity and immune-persistence of the CoronaVac or Covilo inactivated COVID-19 Vaccine: a 6-month population-based cohort study

Frontiers in Immunology, Год журнала: 2022, Номер 13

Опубликована: Авг. 12, 2022

Background Owing to the coronavirus disease 2019 (COVID-19) pandemic and emergency use of different types COVID-19 vaccines, there is an urgent need consider effectiveness persistence vaccines. Methods We investigated immunogenicity CoronaVac Covilo, two inactivated vaccines against that each contain severe acute respiratory syndrome 2 (SARS-CoV-2). The levels neutralizing antibodies live SARS-CoV-2 inhibition rates pseudovirus, as well immunoglobulin (Ig)G IgM responses towards spike (S) nucleocapsid (N) protein at 180 days after two-dose vaccination were detected. Results Covilo induced similar antibody responses. Regarding SARS-CoV-2, 77.9% vaccine recipients 78.3% (aged 18–59 years) seroconverted by 28 second dose. SARS-CoV-2-specific antibodies, 97.1% 95.7% a pseudovirus Delta variant significantly lower compared with those wildtype SARS-CoV-2. Associated participant characteristics levels, persons in older age group basic disease, especially chronic tended have anti-SARS-CoV-2 seroconversion rates. Conclusion Antibodies elicited these appeared wane following their peak dose, but they persisted detectable through 6 months was than which suggests attention must be paid protective effectiveness, its persistence, on variants.

Язык: Английский

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Label-free optical biosensors in the pandemic era

Nanophotonics, Год журнала: 2022, Номер 11(18), С. 4159 - 4181

Опубликована: Авг. 11, 2022

The research in the field of optical biosensors is continuously expanding, thanks both to introduction brand new technologies and ingenious use established methods. A awareness on potential societal impact this has arisen as a consequence Covid-19 pandemic. availability generation analytical tools enabling more accurate understanding bio-molecular processes or development distributed diagnostic devices with improved performance now greater demand clearly envisioned, but not yet achieved. In review, we focus emerging innovation opportunities conveyed by label-free biosensors. We review most recent innovations biosensor technology consideration their competitive selected application areas. operational simplicity implicit detection can be exploited novel rapid compact for applications. adaptability any molecular recognition conformational process facilitates integration DNA nanostructures carrying functions. high sensitivity nanoscale objects stimulates ultrasensitive systems down digital single binding events enhanced nanoparticles direct enumeration bio-nanoparticles like viruses.

Язык: Английский

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Clinical Application of Antibody Immunity Against SARS-CoV-2: Comprehensive Review on Immunoassay and Immunotherapy

Clinical Reviews in Allergy & Immunology, Год журнала: 2022, Номер 64(1), С. 17 - 32

Опубликована: Янв. 15, 2022

Язык: Английский

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Flexible Terahertz Metamaterial Biosensor for Ultra-Sensitive Detection of Hepatitis B Viral DNA Based on the Metal-Enhanced Sandwich Assay

Frontiers in Bioengineering and Biotechnology, Год журнала: 2022, Номер 10

Опубликована: Авг. 5, 2022

The high sensitivity and specificity of terahertz (THz) biosensing are both promising challenging in DNA sample detection. This study produced refined a flexible THz MM biosensor for ultrasensitive detection HBV clinical serum samples based on gold magnetic nanoparticle-mediated rolling circle amplification (GMNPs@RCA) sandwich assay under isothermal conditions. Typically, solid-phase RCA reactions mediated by circular padlock probes (PLPs) triggered conditions the presence DNA, resulting long single-stranded (ssDNA) with fidelity specificity. Then, resultant ssDNA was conjugated (DPs) immobilized nanoparticles (DP@AuNPs) to form GMNPs-RCA-AuNPs complexes. concentrations were quantified introducing complexes into metasurface metamaterial-based chip red shift resonance peak metamaterials. can lead highly specific sensitive one-base mismatch discrimination limit (LOD) down 1.27E + 02 IU/ml from samples. concentration linearly correlated frequency metamaterials within range 02∼1.27E 07 IU/ml, illustrating applicability accuracy our real strategy constitutes sensing method identify virus DNA. In future, it is hoped assist pathogen identification diagnosis.

Язык: Английский

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