Research Square (Research Square),
Год журнала:
2022,
Номер
unknown
Опубликована: Сен. 22, 2022
Abstract
In
vitro
gamma
ray
(60Co)
mutagenesis
is
a
powerful
tool
to
achieve
variability
in
commercial
rice
lines
used
by
farmers,
such
as
Lazarroz
FL.
We
previously
reported
the
optimized
system
for
FL
Indica
callus.
As
continuation,
present
study,
we
targeted
ACC2
gene
that
provides
tolerance
aryloxy-phenoxy-propionate
(APP)
fluazifop-P-butyl
model
show
system's
potential
create
while
providing
solution
weed
management.
The
DL50
of
was
calculated
calli
DL50=
6,93
mg/L
(0,425
-
15,743
mg/L,
R2
=
0,402,
1000n)
and
regenerated
vitroplants
at
an
LD50
3.771
(R2
1,
290n).
5
selection
agent
second
round
10
(3000
)
resulted
one
survivor
plant
when
using
starting
material.
putative
tolerant
also
tolerated
150
greenhouse.
sequenced,
heteroecious
mutation,
T2222I/T2222M,
discovered
may
be
linked
tolerance.
improved
seeds
irradiation
point
instead
embryogenic
calli,
followed
induction,
regeneration,
exposure
agent.
modification
allowed
higher
doses
with
350
Gy
thirty-one
plants.
showed
gamma-ray
mutants
from
recalcitrant
indica
materials
are
possible,
use
agents
APP
can
help
useful
breeding
more
resilient
rice.
Plants,
Год журнала:
2022,
Номер
11(18), С. 2386 - 2386
Опубликована: Сен. 13, 2022
Rice
production
needs
to
be
sustained
in
the
coming
decades,
as
changeable
climatic
conditions
are
becoming
more
conducive
disease
outbreaks.
The
majority
of
rice
diseases
cause
enormous
economic
damage
and
yield
instability.
Among
them,
blast
caused
by
Magnaportheoryzae
is
a
serious
fungal
considered
one
major
threats
world
production.
This
pathogen
can
infect
above-ground
tissues
plants
at
any
growth
stage
causes
complete
crop
failure
under
favorable
conditions.
Therefore,
management
essentially
required
sustain
global
food
When
looking
drawback
chemical
strategy,
development
durable,
resistant
varieties
most
sustainable,
economic,
environment-friendly
approaches
counter
outbreaks
blasts.
Interestingly,
several
blast-resistant
cultivars
have
been
developed
with
help
breeding
biotechnological
methods.
In
addition,
146
R
genes
identified,
37
among
them
molecularly
characterized
date.
Further,
than
500
loci
identified
for
resistance
which
enhances
resources
developing
through
marker-assisted
selection
(MAS),
backcross
(MABB),
genome
editing
tools.
Apart
from
these,
better
understanding
pathogens,
infection
process
pathogen,
genetics
immune
response
host
plant
very
important
effective
disease.
high
throughput
phenotyping
screening
protocols
played
significant
roles
easy
comprehension
mechanism
spread.
present
review
critically
emphasizes
pathogenesis,
pathogenomics,
techniques,
traditional
molecular
approaches,
transgenic
tools
develop
broad
spectrum
durable
against
rice.
updated
comprehensive
information
presented
this
would
definitely
helpful
researchers,
breeders,
students
planning
execution
program
pathogen.
Plants,
Год журнала:
2022,
Номер
11(17), С. 2273 - 2273
Опубликована: Авг. 31, 2022
Sources
of
new
genetic
variability
have
been
limited
to
existing
germplasm
in
the
past.
Wheat
has
studied
extensively
for
various
agronomic
traits
located
throughout
genome.
The
large
size
chromosomes
and
ability
its
polyploid
genome
tolerate
addition
or
loss
facilitated
rapid
progress
early
study
wheat
genetics
using
cytogenetic
techniques.
At
same
time,
characterization
studies
focused
on
diploid
species,
with
a
small
engineering
procedures
already
developed.
Today,
transformation
gene
editing
offer
attractive
alternatives
conventional
techniques
breeding
because
they
allow
one
more
genes
be
introduced
altered
into
an
elite
cultivar
without
affecting
background.
Recently,
significant
advances
made
regenerating
plant
tissues,
providing
essential
basis
transgenic
plants.
In
addition,
Agrobacterium-mediated,
biolistic,
planta
particle
bombardment
(iPB)
delivery
developed
advanced
development.
As
result,
several
useful
are
now
available
that
transferred
would
helpful
current
traditional
effort
improve
trait
values,
such
as
resistance
abiotic
biotic
factors,
grain
quality,
architecture.
Furthermore,
method
will
significantly
contribute
social
implementation
genome-edited
crops
innovate
pipeline
leverage
unique
climate
adaptations.
Plants,
Год журнала:
2025,
Номер
14(3), С. 425 - 425
Опубликована: Фев. 1, 2025
Mutagenesis
breeding,
combined
with
the
application
of
corresponding
herbicides
to
develop
herbicide-resistant
rice
germplasm,
provides
great
promise
for
management
weeds
and
weedy
rice.
In
this
study,
a
topramezone-resistant
mutant,
TZR1,
was
developed
from
indica
line
Chuangyu
9H
(CY9H)
through
radiation
mutagenesis
topramezone
selection.
Dose–response
curves
revealed
that
resistance
index
TZR1
1.94-fold
compared
CY9H.
The
mechanism
not
due
target-site
resistance.
This
could
be
reversed
by
specific
inhibitor
glutathione
S-transferase
(GST).
activity
antioxidant
enzymes
analyzed.
SNPs
Indels
were
detected
using
whole-genome
resequencing;
differentially
expressed
genes
identified
RNA
sequencing.
Then,
they
underwent
Gene
Ontology
Kyoto
Encyclopedia
Genes
Genomes
enrichment
analyses.
Key
candidate
associated
validated
via
real-time
quantitative
PCR
assay.
Five
GST
genes,
two
UDP-glycosyltransferase
three
ATP-binding
cassette
transporter
as
potential
contributors
detoxification
in
TZR1.
Overall,
these
findings
suggest
possibly
play
an
important
role
topramezone.
study
will
provide
valuable
information
scientific
4-hydroxyphenylpyruvate
dioxygenase
inhibitors
paddy
fields
future.
Plants,
Год журнала:
2023,
Номер
12(11), С. 2146 - 2146
Опубликована: Май 29, 2023
Modeling
biological
processes
and
genetic-regulatory
networks
using
in
silico
approaches
provides
a
valuable
framework
for
understanding
how
genes
associated
allelic
genotypic
differences
result
specific
traits.
Submergence
tolerance
is
significant
agronomic
trait
rice;
however,
the
gene–gene
interactions
linked
with
this
polygenic
remain
largely
unknown.
In
study,
we
constructed
network
of
57
transcription
factors
involved
seed
germination
coleoptile
elongation
under
submergence.
The
were
based
on
co-expression
profiles
presence
factor
binding
sites
promoter
region
target
genes.
We
also
incorporated
published
experimental
evidence,
wherever
available,
to
support
gene–gene,
gene–protein,
protein–protein
interactions.
data
obtained
by
re-analyzing
publicly
available
transcriptome
from
rice.
Notably,
includes
OSH1,
OSH15,
OSH71,
Sub1B,
ERFs,
WRKYs,
NACs,
ZFP36,
TCPs,
etc.,
which
play
key
regulatory
roles
germination,
submergence
response,
mediate
gravitropic
signaling
regulating
OsLAZY1
and/or
IL2.
was
manually
biocurated
submitted
Plant
Reactome
Knowledgebase
make
it
accessible.
expect
work
will
facilitate
re-analysis/re-use
OMICs
aid
genomics
research
accelerate
crop
improvement.
Crop and Environment,
Год журнала:
2022,
Номер
1(3), С. 173 - 181
Опубликована: Авг. 17, 2022
Developing
high-yielding
rice
varieties
is
critical
to
ensure
global
food
security.
To
date,
selection
of
promising
genotypes
based
on
empirical
evaluation
for
grain
yield,
but
the
relationship
agronomic
traits
yield
in
tropical
breeding
largely
unknown.
This
study
aimed
determine
extent
variation
19
and
interrelationships
among
an
irrigated
program
at
International
Rice
Research
Institute,
Philippines.
A
large
set
elite
germplasm
comprising
advanced
derived
from
pipeline
was
evaluated
during
dry
wet
seasons.
The
broad-sense
heritability
ranged
0.35
0.99
all
both
Grain
whole
plot
(plot
yield)
positively
correlated
with
per
plant,
1000
weight,
width
season,
width,
number
panicles
panicle
exertion
rate
season.
Path
analysis
showed
that
highest
direct
positive
effect
0.25
0.45
season
0.22
0.88
Heat
map
bi-cluster
assigned
into
three
main
clusters
seasons,
while
were
grouped
five
respectively.
cluster
spikelets
panicle,
filled
grains
plant
key
contributing
traits.
Identification
highly
could
be
useful
developing
new
adapted
environments.
Abstract
Rice
(
Oryza
sativa
L.)
is
one
of
the
most
important
food
crops
worldwide.
The
utilisation
heterosis
(hybrid
vigour)
has
played
a
significant
role
in
increasing
rice
yield
and
ensuring
supply.
Over
past
50
years,
first-generation
three-line
system
based
on
cytoplasmic
male
sterility,
second-generation
two-line
environment-sensitive
genic
sterility
(EGMS),
have
been
widely
applied
hybrid
production.
However,
restricted
by
matching
relationship
among
three
parental
lines
allows
only
~
2–5%
germplasms
to
be
explored
for
elite
combinations.
environmental
sensitivity
EGMS
posed
serious
risks
production
seeds.
These
factors
hindered
development
applications
rice.
Third-generation
technology
(TGHRT)
environment-insensitive
which
can
effectively
overcome
intrinsic
problems
systems.
Since
establishment
TGHRT,
numerous
findings
innovations
reported.
This
paper
gives
brief
review
traditional
technologies
discusses
technical
future
research
that
necessary
promote
wide
application
TGHRT
