Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance

The Science of The Total Environment, Год журнала: 2021, Номер 805, С. 149877 - 149877

Опубликована: Авг. 25, 2021

Wastewater surveillance for pathogens using reverse transcription-polymerase chain reaction (RT-PCR) is an effective and resource-efficient tool gathering community-level public health information, including the incidence of coronavirus disease-19 (COVID-19). Surveillance Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) in wastewater can potentially provide early warning signal COVID-19 infections a community. The capacity world's environmental microbiology virology laboratories SARS-CoV-2 RNA characterization increasing rapidly. However, there are no standardized protocols or harmonized quality assurance control (QA/QC) procedures surveillance. This paper technical review factors that cause false-positive false-negative errors wastewater, culminating recommended strategies be implemented to identify mitigate some these errors. Recommendations include stringent QA/QC measures, representative sampling approaches, virus concentration efficient extraction, PCR inhibition assessment, inclusion sample processing controls, considerations RT-PCR assay selection data interpretation. Clear interpretation guidelines (e.g., determination positive negative samples) critical, particularly when low. Corrective confirmatory actions must place inconclusive results diverging from current trends initial onset reemergence community). It also prudent perform interlaboratory comparisons ensure results' reliability interpretability prospective retrospective analyses. this aim improve detection applications. A silver lining pandemic efficacy continues demonstrated during global crisis. In future, should play important role range other communicable diseases.

Язык: Английский

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Relationships between SARS-CoV-2 in Wastewater and COVID-19 Clinical Cases and Hospitalizations, with and without Normalization against Indicators of Human Waste

ACS ES&T Water, Год журнала: 2022, Номер 2(11), С. 1992 - 2003

Опубликована: Май 26, 2022

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in wastewater has been used to track community infections of coronavirus disease-2019 (COVID-19), providing critical information for public health interventions. Since levels are dependent upon human inputs, we hypothesize that tracking can be improved by normalizing concentrations against indicators waste [Pepper Mild Mottle Virus (PMMoV), β-2 Microglobulin (B2M), and fecal coliform]. In this study, analyzed SARS-CoV-2 from two sewersheds different scales: a University campus treatment plant. Wastewater data were combined with complementary COVID-19 case evaluate the efficiency surveillance forecasting new cases and, larger scale, hospitalizations. Results show normalization PMMoV B2M resulted correlations using volcano second generation (V2G)-qPCR chemistry (rs = 0.69 without normalization, rs 0.73 normalization). Mixed results obtained samples collected at scale. Overall benefits measures depend qPCR improves smaller sewershed We recommend further studies efficacy additional targets.

Язык: Английский

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Opportunities and limits of wastewater-based epidemiology for tracking global health and attainment of UN sustainable development goals

Environment International, Год журнала: 2022, Номер 163, С. 107217 - 107217

Опубликована: Март 27, 2022

Wastewater-based epidemiology (WBE) emerged as a powerful, actionable health management tool during the COVID-19 pandemic. Hypothesizing future uses, we explored its potential for real-time, tracking of progress in attaining United Nations Sustainable Development Goals (SDGs) globally non-expensive method using existing infrastructure. We inventoried (i) literature-documented sewerage infrastructure, (ii) demographics populations served, and (iii) WBE markers informative 9 SDGs. Among 17 different sustainable development goals listed by UN 2030 agenda, more than half these may be monitored monitoring at centralized treatment infrastructure tabulated this study. Driven mainly COVID-19, currently is practiced least 55 countries, reaching about 300 million people. Expansion to 109,000 + plants 129 countries would increase global coverage 9-fold 34.7% or 2.7 billion, leaving out 5 billion people not served systems. Associations between population present-day are explored, geospatial regions particularly vulnerable infectious disease outbreaks identified. The results suggest that difference differential outcomes well-being an outcome sanitation inequalities lack creates doubly disadvantaged risk poor hygiene cut off from early-warning benefits conventional WBE. This first study explore feasibility barriers use attainment SDGs with

Язык: Английский

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First detection of SARS-CoV-2 genetic material in the vicinity of COVID-19 isolation Centre in Bangladesh: Variation along the sewer network

The Science of The Total Environment, Год журнала: 2021, Номер 776, С. 145724 - 145724

Опубликована: Фев. 9, 2021

Язык: Английский

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Analytical methodologies for the detection of SARS-CoV-2 in wastewater: Protocols and future perspectives

TrAC Trends in Analytical Chemistry, Год журнала: 2020, Номер 134, С. 116125 - 116125

Опубликована: Ноя. 20, 2020

In March 2020 the World Health Organization announced a pandemic outbreak. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is causative pathogen for disease-19 (COVID-19) pandemic. The authorities worldwide use clinical science to identify infected people, but this approach not able track all symptomatic and asymptomatic cases due limited sampling capacity of testing laboratories. This drawback eliminated by Wastewater-Based Epidemiology (WBE) approach. review, we summarized peer-reviewed published literature (available as September 28, 2020), in field WBE. commonly used steps (sampling, storage, concentration, isolation, detection) analytical protocols were identified. potential limitations each stage good practices discussed. Finally, new methods efficient detection SARS-CoV-2 proposed.

Язык: Английский

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The COVID-19 pandemic and its implications on the environment

Environmental Research, Год журнала: 2021, Номер 201, С. 111648 - 111648

Опубликована: Июль 7, 2021

Язык: Английский

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A review of the presence of SARS-CoV-2 RNA in wastewater and airborne particulates and its use for virus spreading surveillance

Environmental Research, Год журнала: 2021, Номер 196, С. 110929 - 110929

Опубликована: Фев. 25, 2021

Язык: Английский

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Coronavirus in water media: Analysis, fate, disinfection and epidemiological applications

Journal of Hazardous Materials, Год журнала: 2021, Номер 415, С. 125580 - 125580

Опубликована: Март 5, 2021

Considerable attention has been recently given to possible transmission of SARS-CoV-2 via water media. This review addresses this issue and examines the fate coronaviruses (CoVs) in systems, with particular available information on novel SARS-CoV-2. The methods for determination viable virus particles quantification CoVs and, particular, wastewater are discussed regard concentration emerging detection. analysis environmental stability CoVs, SARS-CoV-2, efficacy disinfection extensively reviewed as well. provides a broad view state-of-the-art researchers involved investigation aquatic poses basis further analyses discussions risk associated presence examined data indicates that detection natural bodies potentially powerful tool quantitative microbiological assessment (QMRA) wastewater-based epidemiology (WBE) evaluation level circulation population. Assays virions media provide integrity, capability replication (in suitable host species) potential infectivity. Challenges critical issues relevant different matrixes both direct surrogate well implementation epidemiological tools presented critically discussed.

Язык: Английский

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Biosensors for the detection of disease outbreaks through wastewater-based epidemiology

TrAC Trends in Analytical Chemistry, Год журнала: 2022, Номер 155, С. 116585 - 116585

Опубликована: Март 7, 2022

Язык: Английский

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A 30-day follow-up study on the prevalence of SARS-COV-2 genetic markers in wastewater from the residence of COVID-19 patient and comparison with clinical positivity

The Science of The Total Environment, Год журнала: 2022, Номер 858, С. 159350 - 159350

Опубликована: Окт. 18, 2022

Wastewater based epidemiology (WBE) is an important tool to fight against COVID-19 as it provides insights into the health status of targeted population from a small single house large municipality in cost-effective, rapid, and non-invasive way. The implementation wastewater surveillance (WBS) could reduce burden on public system, management pandemics, help make informed decisions, protect health. In this study, with patients was for monitoring prevalence SARS-CoV-2 genetic markers samples (WS) clinical specimens (CS) period 30 days. RT-qPCR technique employed target nonstructural (ORF1ab) structural-nucleocapsid (N) protein genes SARS-CoV-2, according validated experimental protocol. Physiological, environmental, biological parameters were also measured following American Public Health Association (APHA) standard protocols. viral shedding peaked when highest number cases clinically diagnosed. Throughout study period, 7450 23,000 gene copies/1000 mL detected, where we identified 47 % (57/120) positive WS 35 (128/360) CS. When patient lowest (2), CT value (39.4; i.e., copy number) WS. On other hand, (6), (25.2 numbers) obtained An advance signal increased load found earlier than Using customized primer sets traditional PCR approach, confirmed that all variants both CS Delta (B.1.617.2). To our knowledge, first follow-up determine temporal relationship between their discharge RNA including family members sampling developing country (Bangladesh), proper sewage system lacking. salient findings indicate virus identify cases, which reduces during pandemics.

Язык: Английский

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