Transmission of SARS-CoV-2 infections and exposure in surfaces, points and wastewaters: A global one health perspective DOI Creative Commons
Dinh‐Toi Chu, Vijai Singh,

Suong-Mai Vu Ngoc

et al.

Case Studies in Chemical and Environmental Engineering, Journal Year: 2022, Volume and Issue: 5, P. 100184 - 100184

Published: Jan. 24, 2022

The persistence of SARS-CoV-2 or its RNA on surfaces, points, wastewaters may increase the risk transmission this virus. Therefore, we conducted review to discuss places and surfaces with highest potential for infection spread Several common public areas, hospitals, elevators, transport, local markets, such as toilets, door handles, untreated treated wastewaters, wastewater plants, washrooms are also considered major points spreading SARS-CoV-2. Highly contaminated often have materials contain items made which virus can persist (e.g., metal, wood, plastic). For example, exist up 4 days doorknobs by those materials. transports, crowding enclosed spaces source transmission. measures using copper alloy instead metal disinfectants, suitable personal protective equipment been suggested. Our research could be basis help develop studies existence transmissibility well take prevent limit harmful effects COVID-19 pandemic.

Language: Английский

Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance DOI Creative Commons
Warish Ahmed, Stuart L. Simpson, Paul M. Bertsch

et al.

The Science of The Total Environment, Journal Year: 2021, Volume and Issue: 805, P. 149877 - 149877

Published: Aug. 25, 2021

Wastewater surveillance for pathogens using reverse transcription-polymerase chain reaction (RT-PCR) is an effective and resource-efficient tool gathering community-level public health information, including the incidence of coronavirus disease-19 (COVID-19). Surveillance Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) in wastewater can potentially provide early warning signal COVID-19 infections a community. The capacity world's environmental microbiology virology laboratories SARS-CoV-2 RNA characterization increasing rapidly. However, there are no standardized protocols or harmonized quality assurance control (QA/QC) procedures surveillance. This paper technical review factors that cause false-positive false-negative errors wastewater, culminating recommended strategies be implemented to identify mitigate some these errors. Recommendations include stringent QA/QC measures, representative sampling approaches, virus concentration efficient extraction, PCR inhibition assessment, inclusion sample processing controls, considerations RT-PCR assay selection data interpretation. Clear interpretation guidelines (e.g., determination positive negative samples) critical, particularly when low. Corrective confirmatory actions must place inconclusive results diverging from current trends initial onset reemergence community). It also prudent perform interlaboratory comparisons ensure results' reliability interpretability prospective retrospective analyses. this aim improve detection applications. A silver lining pandemic efficacy continues demonstrated during global crisis. In future, should play important role range other communicable diseases.

Language: Английский

Citations

226
Relationships between SARS-CoV-2 in Wastewater and COVID-19 Clinical Cases and Hospitalizations, with and without Normalization against Indicators of Human Waste DOI Creative Commons

Qingyu Zhan,

Kristina M. Babler,

Mark Sharkey

et al.

ACS ES&T Water, Journal Year: 2022, Volume and Issue: 2(11), P. 1992 - 2003

Published: May 26, 2022

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in wastewater has been used to track community infections of coronavirus disease-2019 (COVID-19), providing critical information for public health interventions. Since levels are dependent upon human inputs, we hypothesize that tracking can be improved by normalizing concentrations against indicators waste [Pepper Mild Mottle Virus (PMMoV), β-2 Microglobulin (B2M), and fecal coliform]. In this study, analyzed SARS-CoV-2 from two sewersheds different scales: a University campus treatment plant. Wastewater data were combined with complementary COVID-19 case evaluate the efficiency surveillance forecasting new cases and, larger scale, hospitalizations. Results show normalization PMMoV B2M resulted correlations using volcano second generation (V2G)-qPCR chemistry (rs = 0.69 without normalization, rs 0.73 normalization). Mixed results obtained samples collected at scale. Overall benefits measures depend qPCR improves smaller sewershed We recommend further studies efficacy additional targets.

Language: Английский

Citations

82
Opportunities and limits of wastewater-based epidemiology for tracking global health and attainment of UN sustainable development goals DOI Creative Commons
Sangeet Adhikari, Rolf U. Halden

Environment International, Journal Year: 2022, Volume and Issue: 163, P. 107217 - 107217

Published: March 27, 2022

Wastewater-based epidemiology (WBE) emerged as a powerful, actionable health management tool during the COVID-19 pandemic. Hypothesizing future uses, we explored its potential for real-time, tracking of progress in attaining United Nations Sustainable Development Goals (SDGs) globally non-expensive method using existing infrastructure. We inventoried (i) literature-documented sewerage infrastructure, (ii) demographics populations served, and (iii) WBE markers informative 9 SDGs. Among 17 different sustainable development goals listed by UN 2030 agenda, more than half these may be monitored monitoring at centralized treatment infrastructure tabulated this study. Driven mainly COVID-19, currently is practiced least 55 countries, reaching about 300 million people. Expansion to 109,000 + plants 129 countries would increase global coverage 9-fold 34.7% or 2.7 billion, leaving out 5 billion people not served systems. Associations between population present-day are explored, geospatial regions particularly vulnerable infectious disease outbreaks identified. The results suggest that difference differential outcomes well-being an outcome sanitation inequalities lack creates doubly disadvantaged risk poor hygiene cut off from early-warning benefits conventional WBE. This first study explore feasibility barriers use attainment SDGs with

Language: Английский

Citations

76
First detection of SARS-CoV-2 genetic material in the vicinity of COVID-19 isolation Centre in Bangladesh: Variation along the sewer network DOI Open Access
Firoz Ahmed, Md. Aminul Islam, Manish Kumar

et al.

The Science of The Total Environment, Journal Year: 2021, Volume and Issue: 776, P. 145724 - 145724

Published: Feb. 9, 2021

Language: Английский

Citations

100
Analytical methodologies for the detection of SARS-CoV-2 in wastewater: Protocols and future perspectives DOI Creative Commons
‪Nikiforos Alygizakis, Athina Markou, Nikolaos I. Rousis

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2020, Volume and Issue: 134, P. 116125 - 116125

Published: Nov. 20, 2020

In March 2020 the World Health Organization announced a pandemic outbreak. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is causative pathogen for disease-19 (COVID-19) pandemic. The authorities worldwide use clinical science to identify infected people, but this approach not able track all symptomatic and asymptomatic cases due limited sampling capacity of testing laboratories. This drawback eliminated by Wastewater-Based Epidemiology (WBE) approach. review, we summarized peer-reviewed published literature (available as September 28, 2020), in field WBE. commonly used steps (sampling, storage, concentration, isolation, detection) analytical protocols were identified. potential limitations each stage good practices discussed. Finally, new methods efficient detection SARS-CoV-2 proposed.

Language: Английский

Citations

99
The COVID-19 pandemic and its implications on the environment DOI Open Access
Alessio Facciolà, Pasqualina Laganà, Gabriella Caruso

et al.

Environmental Research, Journal Year: 2021, Volume and Issue: 201, P. 111648 - 111648

Published: July 7, 2021

Language: Английский

Citations

69
A review of the presence of SARS-CoV-2 RNA in wastewater and airborne particulates and its use for virus spreading surveillance DOI Open Access

Uttpal Anand,

Bashir Adelodun, Alberto Pivato

et al.

Environmental Research, Journal Year: 2021, Volume and Issue: 196, P. 110929 - 110929

Published: Feb. 25, 2021

Language: Английский

Citations

67
Coronavirus in water media: Analysis, fate, disinfection and epidemiological applications DOI Creative Commons
Antonio Buonerba, Mary Vermi Aizza Corpuz, Florencio Ballesteros

et al.

Journal of Hazardous Materials, Journal Year: 2021, Volume and Issue: 415, P. 125580 - 125580

Published: March 5, 2021

Considerable attention has been recently given to possible transmission of SARS-CoV-2 via water media. This review addresses this issue and examines the fate coronaviruses (CoVs) in systems, with particular available information on novel SARS-CoV-2. The methods for determination viable virus particles quantification CoVs and, particular, wastewater are discussed regard concentration emerging detection. analysis environmental stability CoVs, SARS-CoV-2, efficacy disinfection extensively reviewed as well. provides a broad view state-of-the-art researchers involved investigation aquatic poses basis further analyses discussions risk associated presence examined data indicates that detection natural bodies potentially powerful tool quantitative microbiological assessment (QMRA) wastewater-based epidemiology (WBE) evaluation level circulation population. Assays virions media provide integrity, capability replication (in suitable host species) potential infectivity. Challenges critical issues relevant different matrixes both direct surrogate well implementation epidemiological tools presented critically discussed.

Language: Английский

Citations

60
Biosensors for the detection of disease outbreaks through wastewater-based epidemiology DOI
Mildred G. Jiménez-Rodríguez,

Fernando Silva-Lance,

Lizeth Parra-Arroyo

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2022, Volume and Issue: 155, P. 116585 - 116585

Published: March 7, 2022

Language: Английский

Citations

49
A 30-day follow-up study on the prevalence of SARS-COV-2 genetic markers in wastewater from the residence of COVID-19 patient and comparison with clinical positivity DOI Creative Commons
Md. Aminul Islam, Arifur Rahman, Md. Jakariya

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 858, P. 159350 - 159350

Published: Oct. 18, 2022

Wastewater based epidemiology (WBE) is an important tool to fight against COVID-19 as it provides insights into the health status of targeted population from a small single house large municipality in cost-effective, rapid, and non-invasive way. The implementation wastewater surveillance (WBS) could reduce burden on public system, management pandemics, help make informed decisions, protect health. In this study, with patients was for monitoring prevalence SARS-CoV-2 genetic markers samples (WS) clinical specimens (CS) period 30 days. RT-qPCR technique employed target nonstructural (ORF1ab) structural-nucleocapsid (N) protein genes SARS-CoV-2, according validated experimental protocol. Physiological, environmental, biological parameters were also measured following American Public Health Association (APHA) standard protocols. viral shedding peaked when highest number cases clinically diagnosed. Throughout study period, 7450 23,000 gene copies/1000 mL detected, where we identified 47 % (57/120) positive WS 35 (128/360) CS. When patient lowest (2), CT value (39.4; i.e., copy number) WS. On other hand, (6), (25.2 numbers) obtained An advance signal increased load found earlier than Using customized primer sets traditional PCR approach, confirmed that all variants both CS Delta (B.1.617.2). To our knowledge, first follow-up determine temporal relationship between their discharge RNA including family members sampling developing country (Bangladesh), proper sewage system lacking. salient findings indicate virus identify cases, which reduces during pandemics.

Language: Английский

Citations

45