Cell Reports, Год журнала: 2025, Номер 44(2), С. 115232 - 115232
Опубликована: Янв. 23, 2025
Язык: Английский
Cell Reports, Год журнала: 2025, Номер 44(2), С. 115232 - 115232
Опубликована: Янв. 23, 2025
Язык: Английский
Nature, Год журнала: 2023, Номер 617(7960), С. 360 - 368
Опубликована: Май 3, 2023
Abstract Mapping behavioural actions to neural activity is a fundamental goal of neuroscience. As our ability record large and data increases, there growing interest in modelling dynamics during adaptive behaviours probe representations 1–3 . In particular, although latent embeddings can reveal underlying correlates behaviour, we lack nonlinear techniques that explicitly flexibly leverage joint behaviour uncover 3–5 Here, fill this gap with new encoding method, CEBRA, jointly uses (supervised) hypothesis- or (self-supervised) discovery-driven manner produce both consistent high-performance spaces. We show consistency be used as metric for uncovering meaningful differences, the inferred latents decoding. validate its accuracy demonstrate tool’s utility calcium electrophysiology datasets, across sensory motor tasks simple complex species. It allows single- multi-session datasets hypothesis testing label free. Lastly, CEBRA mapping space, kinematic features, production spaces two-photon Neuropixels data, provide rapid, high-accuracy decoding natural videos from visual cortex.
Язык: Английский
Процитировано
175Nature Communications, Год журнала: 2021, Номер 12(1)
Опубликована: Авг. 27, 2021
To produce consistent sensory perception, neurons must maintain stable representations of input. However, in many regions exhibit progressive drift across days. Longitudinal studies have found responses to artificial stimuli sessions visual areas, but it is unclear whether this stability extends naturalistic stimuli. We performed chronic 2-photon imaging mouse V1 populations directly compare the representational versus over weeks. Responses gratings were highly sessions. neural movies exhibited Differential was present cortical layers, inhibitory interneurons, and could not be explained by differential response strength or higher order stimulus statistics. accompanied similar changes local population correlation structure. These results suggest stimulus-dependent may relate differences preexisting circuit architecture co-tuned neurons.
Язык: Английский
Процитировано
124Current Opinion in Neurobiology, Год журнала: 2022, Номер 76, С. 102609 - 102609
Опубликована: Авг. 5, 2022
Язык: Английский
Процитировано
98Neuron, Год журнала: 2023, Номер 111(15), С. 2357 - 2366.e5
Опубликована: Июнь 13, 2023
Язык: Английский
Процитировано
69Neuron, Год журнала: 2023, Номер 111(15), С. 2348 - 2356.e4
Опубликована: Июнь 13, 2023
Язык: Английский
Процитировано
54Cell, Год журнала: 2023, Номер 186(11), С. 2475 - 2491.e22
Опубликована: Май 1, 2023
Holistic understanding of physio-pathological processes requires noninvasive 3D imaging in deep tissue across multiple spatial and temporal scales to link diverse transient subcellular behaviors with long-term physiogenesis. Despite broad applications two-photon microscopy (TPM), there remains an inevitable tradeoff among spatiotemporal resolution, volumes, durations due the point-scanning scheme, accumulated phototoxicity, optical aberrations. Here, we harnessed concept synthetic aperture radar TPM achieve aberration-corrected dynamics at a millisecond scale for over 100,000 large volumes tissue, three orders magnitude reduction photobleaching. With its advantages, identified direct intercellular communications through migrasome generation following traumatic brain injury, visualized formation process germinal center mouse lymph node, characterized heterogeneous cellular states visual cortex, opening up horizon intravital understand organizations functions biological systems holistic level.
Язык: Английский
Процитировано
52Nature Neuroscience, Год журнала: 2024, Номер 27(3), С. 561 - 572
Опубликована: Янв. 19, 2024
Episodic memories are encoded by experience-activated neuronal ensembles that remain necessary and sufficient for recall. However, the temporal evolution of memory engrams after initial encoding is unclear. In this study, we employed computational experimental approaches to examine how neural composition selectivity change with consolidation. Our spiking network model yielded testable predictions: transition from unselective selective as neurons drop out into engrams; inhibitory activity during recall essential selectivity; synaptic plasticity consolidation critical become selective. Using activity-dependent labeling, longitudinal calcium imaging a combination optogenetic chemogenetic manipulations in mouse dentate gyrus, conducted contextual fear conditioning experiments supported our model's predictions. results reveal dynamic changes engram mediated crucial emergence selectivity.
Язык: Английский
Процитировано
24eLife, Год журнала: 2021, Номер 10
Опубликована: Июль 30, 2021
Neuronal ensembles, coactive groups of neurons found in spontaneous and evoked cortical activity, are causally related to memories perception, but it is still unknown how stable or flexible they over time. We used two-photon multiplane calcium imaging track weeks the activity same pyramidal layer 2/3 visual cortex from awake mice recorded their visually responses. Less than half remained active across any two sessions. These formed ensembles that lasted weeks, some were also transient appeared only one single session. Stable preserved most for up 46 days, our longest imaged period, these 'core' cells had stronger functional connectivity. Our results demonstrate neuronal can last could, principle, serve as a substrate long-lasting representation perceptual states memories.
Язык: Английский
Процитировано
67Nature, Год журнала: 2022, Номер 605(7911), С. 713 - 721
Опубликована: Май 19, 2022
Язык: Английский
Процитировано
60eLife, Год журнала: 2022, Номер 11
Опубликована: Авг. 30, 2022
Neuronal responses to similar stimuli change dynamically over time, raising the question of how internal representations can provide a stable substrate for neural coding. Recent work has suggested large degree drift in even sensory cortices, which are believed store external world. While these is mostly characterized relation stimuli, behavioural state animal (for instance, level arousal) also known strongly modulate activity. We therefore asked variability such modulatory mechanisms contribute representational changes. analysed large-scale recording activity from Allen Brain Observatory, was used before document mouse visual cortex. found that, within datasets, significantly contributes This effect broadcasted across various cortical areas mouse, including primary cortex, higher order areas, and regions not primarily linked vision like hippocampus. Our computational modelling suggests that results consistent with independent modulation by behaviour slower timescales. Importantly, our analysis reliable but variable be misinterpreted as if neuronal only stimulus space marginalized parameters.
Язык: Английский
Процитировано
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