Neuronal wiring diagram of an adult brain

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Июнь 30, 2023

Abstract Connections between neurons can be mapped by acquiring and analyzing electron microscopic (EM) brain images. In recent years, this approach has been applied to chunks of brains reconstruct local connectivity maps that are highly informative, yet inadequate for understanding function more globally. Here, we present the first neuronal wiring diagram a whole adult brain, containing 5×10 7 chemical synapses ∼130,000 reconstructed from female Drosophila melanogaster . The resource also incorporates annotations cell classes types, nerves, hemilineages, predictions neurotransmitter identities. Data products available download, programmatic access, interactive browsing made interoperable with other fly data resources. We show how derive projectome, map projections regions, connectome. demonstrate tracing synaptic pathways analysis information flow inputs (sensory ascending neurons) outputs (motor, endocrine, descending neurons), across both hemispheres, central optic lobes. Tracing subset photoreceptors all way motor illustrates structure uncover putative circuit mechanisms underlying sensorimotor behaviors. technologies open ecosystem FlyWire Consortium set stage future large-scale connectome projects in species.

Язык: Английский

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A searchable image resource of Drosophila GAL4 driver expression patterns with single neuron resolution

eLife, Год журнала: 2023, Номер 12

Опубликована: Фев. 23, 2023

Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of

Язык: Английский

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NeuronBridge: an intuitive web application for neuronal morphology search across large data sets

BMC Bioinformatics, Год журнала: 2024, Номер 25(1)

Опубликована: Март 15, 2024

Abstract Background Neuroscience research in Drosophila is benefiting from large-scale connectomics efforts using electron microscopy (EM) to reveal all the neurons a brain and their connections. To exploit this knowledge base, researchers relate connectome’s structure neuronal function, often by studying individual neuron cell types. Vast libraries of fly driver lines expressing fluorescent reporter genes sets have been created imaged confocal light (LM), enabling targeting for experimentation. However, creating line driving gene expression within single found an EM connectome remains challenge, as it typically requires identifying pair where only interest expressed both. This task other emerging scientific workflows require finding similar across large data different modalities. Results Here, we present NeuronBridge, web application easily rapidly putative morphological matches between We describe functionality construction NeuronBridge service, including its user-friendly graphical user interface (GUI), extensible model, serverless cloud architecture, massively parallel image search engine. Conclusions fills critical gap workflow used hundreds neuroscience around world. offer our software code, open APIs, processed integration reuse, provide service at http://neuronbridge.janelia.org .

Язык: Английский

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Descending neuron population dynamics during odor-evoked and spontaneous limb-dependent behaviors

eLife, Год журнала: 2022, Номер 11

Опубликована: Окт. 25, 2022

Deciphering how the brain regulates motor circuits to control complex behaviors is an important, long-standing challenge in neuroscience. In fly, Drosophila melanogaster , this coordinated by a population of ~ 1100 descending neurons (DNs). Activating only few DNs known be sufficient drive like walking and grooming. However, what additional role larger plays during natural remains largely unknown. For example, they may modulate core behavioral commands or comprise parallel pathways that are engaged depending on sensory context. We evaluated these possibilities recording populations nearly 100 individual tethered flies while generated limb-dependent behaviors, including found largest fraction recorded encode fewer active head grooming resting. A large walk-encoding turning far weakly speed. Although odor context does not determine which behavior-encoding recruited, odors rather than behaviors. Lastly, we illustrate one can identify from DN recordings using their spatial, functional, morphological properties. These results set stage for comprehensive, population-level understanding brain’s signals regulate actions.

Язык: Английский

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A highly conserved A-to-I RNA editing event within the glutamate-gated chloride channel GluClα is necessary for olfactory-based behaviors in Drosophila

Science Advances, Год журнала: 2024, Номер 10(36)

Опубликована: Сен. 4, 2024

A-to-I RNA editing is a cellular mechanism that generates transcriptomic and proteomic diversity, which essential for neuronal immune functions. It involves the conversion of specific adenosines in molecules to inosines, are recognized as guanosines by machinery. Despite vast number sites observed across animal kingdom, pinpointing critical understanding their vivo functions remains challenging. Here, we study function an evolutionary conserved site Drosophila , located glutamate-gated chloride channel ( GluCl α). Our findings reveal flies lacking at this exhibit reduced olfactory responses odors impaired pheromone-dependent social interactions. Moreover, demonstrate crucial proper processing information projection neurons. results highlight value using conservation criterion identifying events with potential functional significance paves way elucidating intricate link between modification, physiology, behavior.

Язык: Английский

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Descending control of motor sequences in

Current Opinion in Neurobiology, Год журнала: 2023, Номер 84, С. 102822 - 102822

Опубликована: Дек. 13, 2023

The descending neurons connecting the fly's brain to its ventral nerve cord respond sensory stimuli and evoke motor programs of varying complexity. Anatomical characterization their synaptic connections suggests how these circuits organize movements, while optogenetic manipulation activity reveals what behaviors they can induce. Monitoring responses or during behavior performance indicates information may encode. Recent advances in all three approaches make an excellent place better understand sensorimotor integration transformation required for nervous systems govern sequences that constitute animal behavior.

Язык: Английский

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NeuronBridge: an intuitive web application for neuronal morphology search across large data sets

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Июль 21, 2022

Abstract Neuroscience research in Drosophila is benefiting from large-scale connectomics efforts using electron microscopy (EM) to reveal all the neurons a brain and their connections. In order exploit this knowledge base, researchers target individual study function. Therefore, vast libraries of fly driver lines expressing fluorescent reporter genes sets have been created imaged confocal light (LM). However, creating line for driving gene expression within single neuron found EM connectome remains challenge, as it typically requires identifying pair where only interest expressed both. This task other emerging scientific workflows require finding similar across large data different modalities. Here, we present NeuronBridge, web application easily rapidly putative morphological matches between datasets We describe functionality construction NeuronBridge service, including its user-friendly GUI, model, serverless cloud architecture, massively parallel image search engine. openly accessible at http://neuronbridge.janelia.org/ .

Язык: Английский

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A searchable image resource ofDrosophilaGAL4-driver expression patterns with single neuron resolution

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2020, Номер unknown

Опубликована: Май 30, 2020

Abstract Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of Drosophila neuroscience. Neuronal targeting typically documented using light microscopy full GAL4 expression patterns, which generally lack the single-cell resolution required for reliable cell type identification. Here we use stochastic labeling with MultiColor FlpOut approach generate cellular confocal images at large scale. We are releasing aligned 74,000 such adult central nervous systems. An anticipated this resource bridge gap between identified by electron or microscopy. Identifying individual that make up each pattern improves prediction split-GAL4 combinations particular neurons. To end have made searchable on NeuronBridge website. demonstrate potential rapidly effectively identify neuron matches based morphology across imaging modalities datasets.

Язык: Английский

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Homeostatic synaptic plasticity rescues neural coding reliability

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Май 24, 2023

Язык: Английский

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Neuronal mechanisms regulating locomotion in adult Drosophila

Journal of Neuroscience Research, Год журнала: 2024, Номер 102(4)

Опубликована: Апрель 1, 2024

Abstract The coordinated action of multiple leg joints and muscles is required even for the simplest movements. Understanding neuronal circuits mechanisms that generate precise movements essential comprehending basis locomotion to infer underlying several locomotor‐related diseases. Drosophila melanogaster provides an excellent model system investigating motor behaviors due its simple nervous genetic accessibility. This review discusses current methods studying locomotor their function in adult . We highlight recently identified pathways modulate distinct forward backward describe control swing stance phases freely moving flies. also report various automated tracking measure motion parameters define inter‐leg coordination, gait speed Finally, we emphasize role proprioceptive signals central coordination. Together, this highlights utility as a uncover circuitry functional organization governs correct movement.

Язык: Английский

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