Techniques
that
enable
precise
manipulations
of
subsets
neurons
in
the
fly
central
nervous
system
have
greatly
facilitated
our
understanding
neural
basis
behavior.
Split-GAL4
driver
lines
allow
specific
targeting
cell
types
Drosophila
melanogaster
and
other
species.
We
describe
here
a
collection
3060
range
adult
1373
characterized
third-instar
larvae.
These
tools
functional,
transcriptomic,
proteomic
studies
based
on
anatomical
targeting.
NeuronBridge
search
relate
light
microscopy
images
these
split-GAL4
to
connectomes
reconstructed
from
electron
images.
The
collections
are
result
screening
over
77,000
split
hemidriver
combinations.
In
addition
stocks
for
well-characterized
lines,
we
make
available
300,000
new
3D
lines.
PLoS ONE,
Год журнала:
2020,
Номер
15(12), С. e0236495 - e0236495
Опубликована: Дек. 31, 2020
The
fruit
fly
Drosophila
melanogaster
is
an
important
model
organism
for
neuroscience
with
a
wide
array
of
genetic
tools
that
enable
the
mapping
individual
neurons
and
neural
subtypes.
Brain
templates
are
essential
comparative
biological
studies
because
they
analyzing
many
individuals
in
common
reference
space.
Several
central
brain
exist
Drosophila,
but
every
one
either
biased,
uses
sub-optimal
tissue
preparation,
imaged
at
low
resolution,
or
does
not
account
artifacts.
No
publicly
available
ventral
nerve
cord
template
currently
exists.
In
this
work,
we
created
high-resolution
using
best-available
technologies
imaging,
artifact
correction,
stitching,
construction
groupwise
registration.
We
evaluated
our
against
four
most
competitive,
demonstrate
ours
enables
more
accurate
registration
fewer
local
deformations
shorter
time.
Current Opinion in Insect Science,
Год журнала:
2022,
Номер
54, С. 100968 - 100968
Опубликована: Сен. 13, 2022
Methods
to
acquire
and
process
synaptic-resolution
electron-microscopy
datasets
have
progressed
very
rapidly,
allowing
production
annotation
of
larger,
more
complete
connectomes.
More
accurate
neuronal
matching
techniques
are
enriching
cell
type
data
with
gene
expression,
neuron
activity,
behaviour
developmental
information,
providing
ways
test
hypotheses
circuit
function.
In
a
variety
behaviours
such
as
learned
innate
olfaction,
navigation
sexual
behaviour,
connectomics
has
already
revealed
interconnected
modules
hierarchical
structure,
recurrence
integration
sensory
streams.
Comparing
individual
connectomes
determine
which
features
robust
variable
is
one
key
research
area;
new
work
in
comparative
across
development,
experience,
sex
species
will
establish
strong
links
between
connectivity
brain
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 10, 2024
Abstract
Techniques
that
enable
precise
manipulations
of
subsets
neurons
in
the
fly
central
nervous
system
have
greatly
facilitated
our
understanding
neural
basis
behavior.
Split-GAL4
driver
lines
allow
specific
targeting
cell
types
Drosophila
melanogaster
and
other
species.
We
describe
here
a
collection
3060
range
adult
1373
characterized
third-instar
larvae.
These
tools
functional,
transcriptomic,
proteomic
studies
based
on
anatomical
targeting.
NeuronBridge
search
relate
light
microscopy
images
these
split-GAL4
to
connectomes
reconstructed
from
electron
images.
The
collections
are
result
screening
over
77,000
split
hemidriver
combinations.
Previously
published
new
included,
all
validated
for
expression
curated
optimal
type
specificity
across
diverse
types.
In
addition
stocks
well-characterized
lines,
we
make
available
300,000
3D
lines.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 6, 2024
In
most
complex
nervous
systems
there
is
a
clear
anatomical
separation
between
the
nerve
cord,
which
contains
of
final
motor
outputs
necessary
for
behaviour,
and
brain.
insects,
neck
connective
both
physical
information
bottleneck
connecting
brain
ventral
cord
(VNC,
spinal
analogue)
comprises
diverse
populations
descending
(DN),
ascending
(AN)
sensory
neurons,
are
crucial
sensorimotor
signalling
control.
Integrating
three
separate
EM
datasets,
we
now
provide
complete
connectomic
description
neurons
female
system
Proceedings of the National Academy of Sciences,
Год журнала:
2024,
Номер
121(10)
Опубликована: Фев. 27, 2024
Connectomics
research
has
made
it
more
feasible
to
explore
how
neural
circuits
can
generate
multiple
outputs.
Female
sexual
drive
provides
a
good
model
for
understanding
reversible,
long-term
functional
changes
in
motivational
circuits.
After
emerging,
female
flies
avoid
male
courtship,
but
they
become
sexually
receptive
over
2
d.
Mating
causes
females
reject
further
mating
several
days.
Here,
we
report
that
pC1
neurons,
which
process
courtship
and
regulate
copulation
behavior,
exhibit
increased
CREB
(cAMP
response
element
binding
protein)
activity
during
maturation
decreased
after
mating.
This
requires
the
neuropeptide
Dh44
(Diuretic
hormone
44)
its
receptors.
A
subset
of
neurons
secretes
Dh44,
stimulates
increases
expression
TRP
channel
Pyrexia
(Pyx)
neurons.
This,
turn,
excitability
drive.
suppresses
pyx
excitability.
is
orthologous
conserved
corticotrophin-releasing
family,
suggesting
similar
roles
other
species.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 2, 2024
Abstract
Locomotion
steering
control
enables
animals
to
pursue
targets,
evade
threats,
avoid
obstacles,
and
explore
their
environment.
Steering
commands
are
generated
in
the
brain
communicated
via
descending
neurons
leg
or
wing
motor
circuits.
The
diversity
of
ways
which
turns
triggered
executed
has
led
view
that
might
rely
on
distributed
neural
processing
across
multiple
Here,
however,
we
present
evidence
for
a
central
circuit
Drosophila
is
used
both
goal-directed
exploratory
capable
eliciting
ranging
from
subtle
course
corrections
rapid
saccades.
organized
hierarchy,
top
layer
comprises
reciprocally
connected
DNa03
LAL013
neurons.
Our
data
suggest
initiated
by
reinforced
stabilized
through
winner-take-all
mechanism
involving
LAL013.
DNa11
form
an
intermediate
layer.
They
receive
input
target
circuits
directly
as
well
indirectly
subordinate
activation
coordinately
changes
stepping
directions
all
six
legs
generate
saccadic
turns.
Together,
these
define
flexibly
fly
exploits
explores
its
Mated
females
reallocate
resources
to
offspring
production,
causing
changes
nutritional
requirements
and
challenges
energy
homeostasis.
Although
observed
across
species,
the
neural
endocrine
mechanisms
that
regulate
needs
of
mated
are
not
well
understood.
Here,
we
find
