Neural signal propagation atlas of Caenorhabditis elegans

Nature, Год журнала: 2023, Номер 623(7986), С. 406 - 414

Опубликована: Ноя. 1, 2023

Abstract Establishing how neural function emerges from network properties is a fundamental problem in neuroscience 1 . Here, to better understand the relationship between structure and of nervous system, we systematically measure signal propagation 23,433 pairs neurons across head nematode Caenorhabditis elegans by direct optogenetic activation simultaneous whole-brain calcium imaging. We sign (excitatory or inhibitory), strength, temporal causal direction these create functional atlas. find that differs model predictions are based on anatomy. Using mutants, show extrasynaptic signalling not visible anatomy contributes this difference. identify many instances dense-core-vesicle-dependent signalling, including timescales less than second, evoke acute transients—often where no wired connection exists but relevant neuropeptides receptors expressed. propose that, such cases, extrasynaptically released serve similar classical neurotransmitters. Finally, our measured atlas predicts dynamics spontaneous activity do models conclude both synaptic drive short timescales, measurements evoked crucial for interpreting function.

Язык: Английский

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Nonlocal Models in Biology and Life Sciences: Sources, Developments, and Applications

Physics of Life Reviews, Год журнала: 2025, Номер 53, С. 24 - 75

Опубликована: Фев. 27, 2025

Язык: Английский

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Deep learning-based aberration compensation improves contrast and resolution in fluorescence microscopy

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 2, 2025

Язык: Английский

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A single neuron in C. elegans orchestrates multiple motor outputs through parallel modes of transmission

Current Biology, Год журнала: 2023, Номер 33(20), С. 4430 - 4445.e6

Опубликована: Сен. 27, 2023

Animals generate a wide range of highly coordinated motor outputs, which allows them to execute purposeful behaviors. Individual neurons in the circuits that behaviors have remarkable capacity for flexibility as they exhibit multiple axonal projections, transmitter systems, and modes neural activity. How these multi-functional properties enable generation adaptive remains unknown. Here, we show HSN neuron C. elegans evokes programs over different timescales suite behavioral changes during egg laying. Using activity perturbations vivo calcium imaging, acutely increases laying locomotion while also biasing animals toward low-speed dwelling behavior minutes. The acute effects on high-speed are mediated by separate sets transmitters compartments. long-lasting part release serotonin, is taken up re-released NSM, another serotonergic class directly dwelling. Our results how single allow it induce reveal can borrow serotonin from one control behavior.

Язык: Английский

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Neuropeptide signaling network of Caenorhabditis elegans: from structure to behavior

Genetics, Год журнала: 2024, Номер unknown

Опубликована: Сен. 30, 2024

Abstract Neuropeptides are abundant signaling molecules that control neuronal activity and behavior in all animals. Owing part to its well-defined compact nervous system, Caenorhabditis elegans has been one of the primary model organisms used investigate how neuropeptide networks organized these neurochemicals regulate behavior. We here review recent work expanded our understanding neuropeptidergic network C. by mapping evolutionary conservation, molecular expression, receptor–ligand interactions, system-wide organization pathways system. also describe general insights into circuit motifs spatiotemporal range peptidergic transmission have emerged from vivo studies on signaling. With efforts ongoing chart peptide other organisms, connectome can serve as a prototype further understand dynamics at organismal level.

Язык: Английский

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Hub overload and failure as a final common pathway in neurological brain network disorders

Network Neuroscience, Год журнала: 2023, Номер 8(1), С. 1 - 23

Опубликована: Окт. 6, 2023

Abstract Understanding the concept of network hubs and their role in brain disease is now rapidly becoming important for clinical neurology. Hub nodes networks are areas highly connected to rest brain, which handle a large part all traffic. They also show high levels neural activity metabolism, makes them vulnerable many different types pathology. The present review examines recent evidence prevalence nature hub involvement variety neurological disorders, emphasizing common themes across In focal epilepsy, pathological may play spreading seizure activity, removal such associated with improved outcome. stroke, damage impaired cognitive recovery. Breakdown optimal organization multiple sclerosis accompanied by dysfunction. Alzheimer’s disease, hyperactive directly amyloid-beta tau Early reliable detection pathology disturbed connectivity imaging neurophysiological techniques opens up opportunities detect patients hyperexcitability profile, who could benefit from treatment anti-epileptic drugs.

Язык: Английский

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Building and integrating brain-wide maps of nervous system function in invertebrates

Current Opinion in Neurobiology, Год журнала: 2024, Номер 86, С. 102868 - 102868

Опубликована: Апрель 3, 2024

The selection and execution of context-appropriate behaviors is controlled by the integrated action neural circuits throughout brain. However, how activity coordinated across brain regions, nervous system structure enables these functional interactions, remain open questions. Recent technical advances have made it feasible to build brain-wide maps function, such as maps, connectomes, cell atlases. Here, we review recent progress in this area, focusing on C. elegans D. melanogaster, work has produced global systems. We also describe circuit motifs elucidated studies specific networks, which highlight complexities that must be captured accurate models whole-brain function.

Язык: Английский

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Unifying community whole-brain imaging datasets enables robust neuron identification and reveals determinants of neuron position in C. elegans

Cell Reports Methods, Год журнала: 2025, Номер unknown, С. 100964 - 100964

Опубликована: Янв. 1, 2025

Язык: Английский

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Simple Worm, Complex Disorder: Studying Down Syndrome Mechanisms in the Unassuming Model C. elegans

Опубликована: Янв. 1, 2025

Язык: Английский

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Hierarchical communities in the larval Drosophila connectome: Links to cellular annotations and network topology

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(38)

Опубликована: Сен. 13, 2024

One of the longstanding aims network neuroscience is to link a connectome’s topological properties—i.e., features defined from connectivity alone–with an organism’s neurobiology. approach for doing so compare connectome properties with annotational maps. This type analysis popular at meso-/macroscale, but less common nano-scale, owing paucity neuron-level data. However, recent methodological advances have made possible reconstruction whole-brain connectomes single-neuron resolution select set organisms. These include fruit fly, Drosophila melanogaster , and its developing larvae. In addition fine-scale descriptions connectivity, these datasets are accompanied by rich annotations. Here, we use variant stochastic blockmodel detect multilevel communities in larval connectome. We find that partition neurons based on function cell most interact assortatively, reflecting principle functional segregation. small number nonassortatively, forming form “rich-club” interneurons receive sensory/ascending inputs deliver outputs along descending pathways. Next, investigate role community structure shaping communication patterns. polysynaptic signaling follows specific trajectories across modular hierarchies, playing key mediating routes between modules hierarchical scales. Our work suggests relationship system-level architecture biological classification individual neurons. envision our study as important step toward bridging gap complex systems neurobiological lines investigation brain sciences.

Язык: Английский

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