See Elegans: Simple-to-use, accurate, and automatic 3D detection of neural activity from densely packed neurons DOI Creative Commons
Enrico Lanza,

Valeria Lucente,

Martina Nicoletti

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(3), P. e0300628 - e0300628

Published: March 22, 2024

In the emerging field of whole-brain imaging at single-cell resolution, which represents one new frontiers to investigate link between brain activity and behavior, nematode Caenorhabditis elegans offers most characterized models for systems neuroscience. Whole-brain recordings consist 3D time series volumes that need be processed obtain neuronal traces. Current solutions this task are either computationally demanding or limited specific acquisition setups. Here, we propose See Elegans, a direct programming algorithm combines different techniques automatic neuron segmentation tracking without RFP channel, compare it with other available algorithms. While outperforming them in cases, our solution novel method guide identification subset head neurons based on position activity. The built-in interface allows user follow manually curate each processing steps. Elegans is thus simple-to-use aimed speeding up post-processing volumetric calcium while maintaining high level accuracy low computational demands. (Contact: [email protected] ).

Language: Английский

Neural signal propagation atlas of Caenorhabditis elegans DOI Creative Commons
Francesco Randi, Anuj Kumar Sharma, Sophie Dvali

et al.

Nature, Journal Year: 2023, Volume and Issue: 623(7986), P. 406 - 414

Published: Nov. 1, 2023

Abstract Establishing how neural function emerges from network properties is a fundamental problem in neuroscience 1 . Here, to better understand the relationship between structure and of nervous system, we systematically measure signal propagation 23,433 pairs neurons across head nematode Caenorhabditis elegans by direct optogenetic activation simultaneous whole-brain calcium imaging. We sign (excitatory or inhibitory), strength, temporal causal direction these create functional atlas. find that differs model predictions are based on anatomy. Using mutants, show extrasynaptic signalling not visible anatomy contributes this difference. identify many instances dense-core-vesicle-dependent signalling, including timescales less than second, evoke acute transients—often where no wired connection exists but relevant neuropeptides receptors expressed. propose that, such cases, extrasynaptically released serve similar classical neurotransmitters. Finally, our measured atlas predicts dynamics spontaneous activity do models conclude both synaptic drive short timescales, measurements evoked crucial for interpreting function.

Language: Английский

Citations

81
Nonlocal Models in Biology and Life Sciences: Sources, Developments, and Applications DOI Creative Commons
Swadesh Pal, Roderick Melnik

Physics of Life Reviews, Journal Year: 2025, Volume and Issue: 53, P. 24 - 75

Published: Feb. 27, 2025

Language: Английский

Citations

3
Deep learning-based aberration compensation improves contrast and resolution in fluorescence microscopy DOI Creative Commons
Min Guo, Yicong Wu, Chad M. Hobson

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 2, 2025

Language: Английский

Citations

2
A single neuron in C. elegans orchestrates multiple motor outputs through parallel modes of transmission DOI Creative Commons
Yung-Chi Huang,

Jinyue Luo,

Wenjia Huang

et al.

Current Biology, Journal Year: 2023, Volume and Issue: 33(20), P. 4430 - 4445.e6

Published: Sept. 27, 2023

Animals generate a wide range of highly coordinated motor outputs, which allows them to execute purposeful behaviors. Individual neurons in the circuits that behaviors have remarkable capacity for flexibility as they exhibit multiple axonal projections, transmitter systems, and modes neural activity. How these multi-functional properties enable generation adaptive remains unknown. Here, we show HSN neuron C. elegans evokes programs over different timescales suite behavioral changes during egg laying. Using activity perturbations vivo calcium imaging, acutely increases laying locomotion while also biasing animals toward low-speed dwelling behavior minutes. The acute effects on high-speed are mediated by separate sets transmitters compartments. long-lasting part release serotonin, is taken up re-released NSM, another serotonergic class directly dwelling. Our results how single allow it induce reveal can borrow serotonin from one control behavior.

Language: Английский

Citations

17
Neuropeptide signaling network of Caenorhabditis elegans: from structure to behavior DOI Creative Commons
Jan Watteyne,

Aleksandra Chudinova,

Lidia Ripoll-Sánchez

et al.

Genetics, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 30, 2024

Abstract Neuropeptides are abundant signaling molecules that control neuronal activity and behavior in all animals. Owing part to its well-defined compact nervous system, Caenorhabditis elegans has been one of the primary model organisms used investigate how neuropeptide networks organized these neurochemicals regulate behavior. We here review recent work expanded our understanding neuropeptidergic network C. by mapping evolutionary conservation, molecular expression, receptor–ligand interactions, system-wide organization pathways system. also describe general insights into circuit motifs spatiotemporal range peptidergic transmission have emerged from vivo studies on signaling. With efforts ongoing chart peptide other organisms, connectome can serve as a prototype further understand dynamics at organismal level.

Language: Английский

Citations

5
Hub overload and failure as a final common pathway in neurological brain network disorders DOI Creative Commons
Cornelis J. Stam

Network Neuroscience, Journal Year: 2023, Volume and Issue: 8(1), P. 1 - 23

Published: Oct. 6, 2023

Abstract Understanding the concept of network hubs and their role in brain disease is now rapidly becoming important for clinical neurology. Hub nodes networks are areas highly connected to rest brain, which handle a large part all traffic. They also show high levels neural activity metabolism, makes them vulnerable many different types pathology. The present review examines recent evidence prevalence nature hub involvement variety neurological disorders, emphasizing common themes across In focal epilepsy, pathological may play spreading seizure activity, removal such associated with improved outcome. stroke, damage impaired cognitive recovery. Breakdown optimal organization multiple sclerosis accompanied by dysfunction. Alzheimer’s disease, hyperactive directly amyloid-beta tau Early reliable detection pathology disturbed connectivity imaging neurophysiological techniques opens up opportunities detect patients hyperexcitability profile, who could benefit from treatment anti-epileptic drugs.

Language: Английский

Citations

12
Building and integrating brain-wide maps of nervous system function in invertebrates DOI Creative Commons
Talya S. Kramer, Steven W. Flavell

Current Opinion in Neurobiology, Journal Year: 2024, Volume and Issue: 86, P. 102868 - 102868

Published: April 3, 2024

The selection and execution of context-appropriate behaviors is controlled by the integrated action neural circuits throughout brain. However, how activity coordinated across brain regions, nervous system structure enables these functional interactions, remain open questions. Recent technical advances have made it feasible to build brain-wide maps function, such as maps, connectomes, cell atlases. Here, we review recent progress in this area, focusing on C. elegans D. melanogaster, work has produced global systems. We also describe circuit motifs elucidated studies specific networks, which highlight complexities that must be captured accurate models whole-brain function.

Language: Английский

Citations

4
Unifying community whole-brain imaging datasets enables robust neuron identification and reveals determinants of neuron position in C. elegans DOI Creative Commons
Daniel Sprague, Kevin Rusch, Raymond L. Dunn

et al.

Cell Reports Methods, Journal Year: 2025, Volume and Issue: unknown, P. 100964 - 100964

Published: Jan. 1, 2025

Language: Английский

Citations

0
Simple Worm, Complex Disorder: Studying Down Syndrome Mechanisms in the Unassuming Model C. elegans DOI

Sergio Sánchez,

Jonathan T. Pierce

Published: Jan. 1, 2025

Language: Английский

Citations

0
Hierarchical communities in the larval Drosophila connectome: Links to cellular annotations and network topology DOI Creative Commons
Richard F. Betzel, Maria Grazia Puxeddu, Caio Seguin

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(38)

Published: Sept. 13, 2024

One of the longstanding aims network neuroscience is to link a connectome’s topological properties—i.e., features defined from connectivity alone–with an organism’s neurobiology. approach for doing so compare connectome properties with annotational maps. This type analysis popular at meso-/macroscale, but less common nano-scale, owing paucity neuron-level data. However, recent methodological advances have made possible reconstruction whole-brain connectomes single-neuron resolution select set organisms. These include fruit fly, Drosophila melanogaster , and its developing larvae. In addition fine-scale descriptions connectivity, these datasets are accompanied by rich annotations. Here, we use variant stochastic blockmodel detect multilevel communities in larval connectome. We find that partition neurons based on function cell most interact assortatively, reflecting principle functional segregation. small number nonassortatively, forming form “rich-club” interneurons receive sensory/ascending inputs deliver outputs along descending pathways. Next, investigate role community structure shaping communication patterns. polysynaptic signaling follows specific trajectories across modular hierarchies, playing key mediating routes between modules hierarchical scales. Our work suggests relationship system-level architecture biological classification individual neurons. envision our study as important step toward bridging gap complex systems neurobiological lines investigation brain sciences.

Language: Английский

Citations

2