Neuronal wiring diagram of an adult brain

Nature, Год журнала: 2024, Номер 634(8032), С. 124 - 138

Опубликована: Окт. 2, 2024

Язык: Английский

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Classification and genetic targeting of cell types in the primary taste and premotor center of the adult Drosophila brain

eLife, Год журнала: 2021, Номер 10

Опубликована: Сен. 2, 2021

Neural circuits carry out complex computations that allow animals to evaluate food, select mates, move toward attractive stimuli, and away from threats. In insects, the subesophageal zone (SEZ) is a brain region receives gustatory, pheromonal, mechanosensory inputs contributes control of diverse behaviors, including feeding, grooming, locomotion. Despite its importance in sensorimotor transformations, study SEZ has been hindered by limited knowledge underlying diversity neurons. Here, we generate collection split-GAL4 lines provides precise genetic targeting 138 different cell types adult Drosophila melanogaster, comprising approximately one third all We characterize single-cell anatomy these neurons find they cluster morphology into six supergroups organize discrete anatomical domains. majority local interneurons are not classically polarized, suggesting rich processing, whereas projection tend be conveying information number higher regions. This insight organization generates resources will facilitate further their contributions sensory processing behavior.

Язык: Английский

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retro-Tango enables versatile retrograde circuit tracing in Drosophila

eLife, Год журнала: 2023, Номер 12

Опубликована: Май 11, 2023

Transsynaptic tracing methods are crucial tools in studying neural circuits. Although a couple of anterograde and targeted retrograde tool have been developed Drosophila melanogaster , there is still need for an unbiased, user-friendly, flexible system. Here, we describe retro -Tango, method transsynaptic, circuit manipulation . In this genetically encoded system, ligand-receptor interaction at the synapse triggers intracellular signaling cascade that results reporter gene expression presynaptic neurons. Importantly, panneuronal elements renders versatile, enabling its use not only to test hypotheses but also generate them. We validate -Tango various circuits benchmark it by comparing our findings with electron microscopy reconstruction hemibrain. Our experiments establish as key neuroscience research.

Язык: Английский

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Selective integration of diverse taste inputs within a single taste modality

eLife, Год журнала: 2023, Номер 12

Опубликована: Янв. 24, 2023

A fundamental question in sensory processing is how different channels of input are processed to regulate behavior. Different may converge onto common downstream pathways drive the same behaviors, or they activate separate distinct behaviors. We investigated this Drosophila bitter taste system, which contains diverse bitter-sensing cells residing organs. First, we optogenetically activated subsets neurons within each organ. These elicited broad and highly overlapping behavioral effects, suggesting that pathways, but also observed differences argue for biased convergence. Consistent with these results, transsynaptic tracing revealed organs connect connectivity. one type neuron projects higher brain. integrate from multiple specific taste-related then traced circuits, providing first glimpse into Together, results reveal inputs selectively integrated early circuit, enabling pooling information, while circuit diverges have roles.

Язык: Английский

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Diverse mechanisms of taste coding in Drosophila

Science Advances, Год журнала: 2023, Номер 9(46)

Опубликована: Ноя. 17, 2023

Taste systems encode chemical cues that drive vital behaviors. We have elucidated noncanonical features of taste coding using an unconventional kind electrophysiological analysis. find neurons Drosophila are much more sensitive than previously thought. They a low spontaneous firing frequency depends on receptors. dual function as olfactory neurons: activated by most tested odorants, including N , -diethyl- meta -toluamide (DEET), at distance. DEET can also inhibit certain neurons, revealing there two modes response: activation and inhibition. characterize OFF responses the tastants elicit them related in structure. link tastant identity to behavior: magnitude response elicited correlated with egg laying behavior it elicited. In summary, sensitivity capacity system greater known.

Язык: Английский

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A neuronal substrate for translating nutrient state and resource density estimations into foraging decisions

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Июль 19, 2023

Foraging animals must balance the costs of exploring their surroundings with potential benefits finding nutritional resources. Each time an animal encounters a food source it decide whether to initiate feeding or continue searching for potentially better options. Experimental evidence and patch foraging models predict that this decision depends on both state density available resources in environment. How brain integrates such internal external states adapt so-called exploration-exploitation trade-off remains poorly understood. We use video-based tracking show Drosophila regulates engage patches based travel between patches, latter being measure To uncover neuronal basis process, we performed neurogenetic silencing screen more than 400 genetic driver lines sparse expression patterns fly brain. identified population neurons central complex acts as key regulator patch. manipulating activity these alters probability engage, is modulated by protein animal, perturbs ability adjust decisions fly’s patches. Taken together, our results reveal substrate information control specific decision, therefore provide important step towards mechanistic explanation cognitive computations resolve cost-benefit trade-offs.

Язык: Английский

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Tango-seq: overlaying transcriptomics on connectomics to identify neurons downstream of Drosophila clock neurons

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 22, 2024

Summary Knowing how neural circuits change with neuronal plasticity and differ between individuals is important to fully understand behavior. Connectomes are typically assembled using electron microscopy, but this low throughput impractical for analyzing or mutations. Here, we modified the trans -Tango genetic circuit-tracing technique identify neurons synaptically downstream of Drosophila s-LNv clock neurons, which show 24hr rhythms. target were labeled specifically in adult flies a nuclear reporter gene, facilitated their purification then single cell sequencing. We call Tango-seq, it allows transcriptomic data – thus identity be overlayed on top anatomical data. found that s-LNvs preferentially make synaptic connections subset CNMa+ DN1p these likely plastic connections. also identified mushroom body Kenyon cells. Tango-seq should useful addition connectomics toolkit.

Язык: Английский

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Connectomic analysis of taste circuits in Drosophila

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 12, 2025

Abstract Our sense of taste is critical for regulating food consumption. The fruit fly Drosophila represents a highly tractable model to investigate mechanisms processing, but circuits beyond sensory neurons are largely unidentified. Here, we use whole-brain connectome the organization circuits. We trace pathways from four populations that detect different modalities and project subesophageal zone (SEZ), primary region brain. find second-order primarily located within SEZ segregated by modality, whereas third-order have more projections outside overlap between modalities. Taste out innervate regions implicated in feeding, olfactory learning. analyze interconnections pathways, characterize modality-dependent differences neuron properties, identify other types inputs onto computational simulations relate neuronal connectivity predicted activity. These studies provide insight into architecture

Язык: Английский

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Artificial sweeteners differentially activate sweet and bitter gustatory neurons in Drosophila

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 6, 2025

ABSTRACT Artificial sweeteners are highly sweet, non-nutritive compounds that have become increasingly popular over recent decades despite research suggesting their consumption has unintended consequences. Specifically, there is evidence some of these chemicals interact with bitter taste receptors, implying likely generate complex chemosensory signals. Here, we report the basic sensory characteristics in Drosophila , a common model system used to study impacts diet, and find all noncaloric inhibited appetitive feeding responses at higher concentrations. At cellular level, found sucralose rebaudioside A co-activated sweet gustatory receptor neurons (GRNs), two populations reciprocally impact behavior, while aspartame only activated cells. We assessed behavioral co-activation low concentrations signal high aversion. Finally, silencing GRNs reduced aversive elicited by significantly increased behaviors. Together, conclude artificial more than “sweetness” alone, this behaviorally relevant effects on may help flies flexibly respond unique compounds.

Язык: Английский

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Functional labeling of individualized postsynaptic neurons using optogenetics and trans-Tango in Drosophila (FLIPSOT)

PLoS Genetics, Год журнала: 2024, Номер 20(3), С. e1011190 - e1011190

Опубликована: Март 14, 2024

A population of neurons interconnected by synapses constitutes a neural circuit, which performs specific functions upon activation. It is essential to identify both anatomical and functional entities circuits comprehend the components processes necessary for healthy brain function changes that characterize disorders. To date, few methods are available study these two aspects circuit simultaneously. In this study, we developed FLIPSOT, or labeling individualized postsynaptic using optogenetics trans- Tango. FLIPSOT uses (1) Tango access genetically, (2) optogenetic approaches activate (FLIPSOTa) inhibit (FLIPSOTi) in random sparse manner, (3) fluorescence markers tagged with genes visualize neurons. Therefore, allows presynaptic driver behavioral individual readily applied has potential be adapted use mammalian circuits.

Язык: Английский

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