bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Июнь 3, 2023
The
maintenance
of
epithelial
barrier
function
involves
cellular
tension,
with
cells
pulling
on
their
neighbors
to
maintain
integrity.
Wounding
interrupts
which
may
serve
as
an
early
signal
initiate
repair.
To
characterize
how
wounds
alter
we
used
a
laser-recoil
assay
map
cortical
tension
around
in
the
monolayer
Drosophila
pupal
notum.
Within
minute
wounding,
there
was
widespread
loss
along
both
radial
and
tangential
directions.
This
similar
levels
observed
Rok
inactivation.
Tension
subsequently
restored
wound,
first
distal
then
proximal
cells,
reaching
wound
margin
about
10
minutes
after
wounding.
Restoring
required
GPCR
Mthl10
IP3
receptor,
indicating
importance
this
calcium
signaling
pathway
known
be
activated
by
damage.
restoration
correlated
inward-moving
contractile
wave
that
has
been
previously
reported;
however,
itself
not
affected
knockdown.
These
results
indicate
transiently
increase
contract
absence
signaling,
but
is
critical
for
fully
resetting
baseline
it
disrupted
Nature Cell Biology,
Год журнала:
2024,
Номер
26(9), С. 1535 - 1544
Опубликована: Июнь 25, 2024
Ferroptosis
is
a
distinct
form
of
necrotic
cell
death
caused
by
overwhelming
lipid
peroxidation,
and
emerging
evidence
indicates
major
contribution
to
organ
damage
in
multiple
pathologies.
However,
ferroptosis
has
not
yet
been
visualized
vivo
due
lack
specific
probes,
which
severely
limited
the
study
how
immune
system
interacts
with
ferroptotic
cells
this
process
contributes
inflammation.
Consequently,
whether
physiological
role
remained
key
outstanding
question.
Here
we
identify
distinct,
ferroptotic-like,
occurring
during
wounding
Drosophila
embryo
using
live
imaging.
We
further
demonstrate
that
macrophages
rapidly
engage
these
within
but
struggle
engulf
them,
leading
prolonged,
frustrated
phagocytosis
frequent
corpse
disintegration.
Conversely,
suppression
programme
delays
macrophage
recruitment
injury
site,
pointing
conflicting
roles
for
inflammation
vivo.
Nature Communications,
Год журнала:
2025,
Номер
16(1)
Опубликована: Янв. 2, 2025
Propagation
of
intercellular
calcium
waves
through
tissues
has
been
found
to
coordinate
different
multicellular
responses.
Nevertheless,
our
understanding
how
operate
remains
limited.
In
this
study,
we
explore
the
real-time
dynamics
in
Drosophila
adipose
tissues.
We
identify
Adipokinetic
Hormone
(AKH),
fly
functional
homolog
glucagon,
as
key
factor
driving
Ca2+
activities
tissue.
find
that
AKH,
which
is
released
into
hemolymph
from
AKH-producing
neurosecretory
cells,
stimulates
larval
fat
by
a
previously
unrecognized
gap-junction-independent
mechanism
promote
lipolysis.
adult
body,
however,
gap-junction-dependent
are
triggered
presumably
uniformly
diffused
AKH.
Additionally,
discover
amino
acids
activate
leading
increased
intracellular
and
AKH
secretion.
Altogether,
show
dietary
regulate
release
cells
brain,
subsequently
tissue,
enhancing
lipid
metabolism.
Intercellular
(ICWs)
responses,
although
precise
mechanisms
less
understood.
Here,
authors
nutrient-triggered
hormone
brain
ICWs
In
multicellular
organisms,
endocrine
factors
such
as
hormones
and
cytokines
regulate
development
homoeostasis
through
communication
between
different
organs.
For
understanding
interorgan
communications
factors,
the
fruit
fly
Drosophila
melanogaster
serves
an
excellent
model
system
due
to
conservation
of
essential
systems
flies
mammals
availability
powerful
genetic
tools.
other
insects,
functions
neuropeptides
or
peptide
from
central
nervous
have
been
extensively
studied.
However,
a
series
recent
studies
conducted
in
revealed
that
derived
peripheral
tissues
also
play
critical
roles
regulating
multiple
biological
processes,
including
growth,
metabolism,
reproduction,
behaviour.
Here,
we
summarise
advances
target
organs/tissues
peripherally
describe
how
these
contribute
various
events
communications.
Cold Spring Harbor Perspectives in Biology,
Год журнала:
2021,
Номер
unknown, С. a040733 - a040733
Опубликована: Дек. 6, 2021
Melanie
I.
Worley
and
Iswar
K.
Hariharan
Department
of
Molecular
Cell
Biology,
University
California,
Berkeley,
California
94720-3200,
USA
Correspondence:
ikh{at}berkeley.edu
Insect Molecular Biology,
Год журнала:
2024,
Номер
33(3), С. 270 - 282
Опубликована: Фев. 8, 2024
Abstract
Insects
rely
on
their
innate
immune
system
to
eliminate
pathogenic
microbes.
As
a
component,
cytokines
transmit
intercellular
signals
control
responses.
Growth‐blocking
peptide
(GBP)
is
member
of
the
stress‐responsive
family
found
in
several
orders
insects,
including
Drosophila
.
However,
physiological
role
GBP
defence
against
pathogens
not
thoroughly
understood.
In
this
study,
we
explored
functions
lepidopteran
pest,
Ostrinia
furnacalis
Injection
recombinant
O.
(
Of
GBP)
precursor
(proGBP)
and
chemically
synthesised
significantly
induced
transcription
antimicrobial
peptides
(AMPs)
other
immunity‐related
genes
deficiency
IMD
)
Dorsal
The
level
mRNA
was
upregulated
after
bacterial
infection.
Knockdown
expression
led
decrease
,
Relish
MyD88
levels.
phenoloxidase
activity
affected
hemocyte
behaviours
larvae.
summary,
potent
cytokine,
effectively
regulating
AMP
synthesis,
melanization
response
cellular
immunity
invading
pathogens.
Molecular Biology of the Cell,
Год журнала:
2024,
Номер
35(5)
Опубликована: Март 27, 2024
The
maintenance
of
epithelial
barrier
function
involves
cellular
tension,
with
cells
pulling
on
their
neighbors
to
maintain
integrity.
Wounding
interrupts
which
may
serve
as
an
early
signal
initiate
repair.
To
characterize
how
wounds
alter
tension
we
used
a
laser-recoil
assay
map
cortical
around
in
the
monolayer
Drosophila
pupal
notum.
Within
minute
wounding,
there
was
widespread
loss
along
both
radial
and
tangential
directions.
This
similar
levels
observed
Rok
inactivation.
Tension
subsequently
restored
wound,
first
distal
then
proximal
cells,
reaching
wound
margin
∼10
min
after
wounding.
Restoring
required
GPCR
Mthl10
IP
3
receptor,
indicating
importance
this
calcium
signaling
pathway
known
be
activated
by
damage.
restoration
correlated
inward-moving
contractile
wave
that
has
been
previously
reported;
however,
itself
not
affected
knockdown.
These
results
indicate
transiently
increase
contract
absence
signaling,
but
is
critical
for
fully
resetting
baseline
it
disrupted
STAR Protocols,
Год журнала:
2022,
Номер
3(2), С. 101396 - 101396
Опубликована: Май 14, 2022
This
protocol
describes
the
preparation
of
Drosophilamelanogaster
pupae
for
laser
ablation
and
live
imaging
notum
(dorsal
thorax).
Because
pupa
is
stationary,
it
can
be
continuously
imaged
multiple
days
if
desired,
making
ideal
studying
wound
signaling
repair,
from
before
through
closure.
In
this
protocol,
we
demonstrate
processes
staging,
partially
dissecting,
mounting,
wounding,
pupal
notum,
with
wounding
occurring
during
process.
For
complete
details
on
use
execution
please
refer
to
O'Connor
et
al.
(2021b).
