The multiple lncRNAs encodinghsrω gene is essential for oogenesis inDrosophila DOI Creative Commons
Rima Saha, S. C. Lakhotia

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Дек. 25, 2022

Abstract In the background of limited studies on noncoding RNAs in Drosophila oogenesis, we show developmentally active hsr ω lncRNA gene to be essential oogenesis and ovulation. The near-null 66 females, ovaries with down- or up-regulated display varyingly perturbed including fewer ovarioles, high apoptosis, poor actin nuclear-cage (stage 10), low Cut levels late chambers and, finally ovulation block. Restoration normal following targeted expression ω-RH transcript confirmed mis-function underlie these defects. Genetic interaction showed varying modulation ovarian defects mis-expression Cut, TBPH/TDP-43 Caz/dFUS hnRNPs by altered levels. Dietary supplement ecdysone which have reduced titer, substantially restored oogenesis. Our results for first time that multiple producing gene, interacts diverse other regulatory molecules, As expected a key roles dynamics various hnRNPs, interactions between down upregulated transcripts regulators are not linear. Summary Statement critically impacts at steps through intra- inter-organ signaling.

Язык: Английский

A gut-derived hormone suppresses sugar appetite and regulates food choice in Drosophila DOI Creative Commons
Alina Malita,

Olga I. Kubrak,

Takashi Koyama

и другие.

Nature Metabolism, Год журнала: 2022, Номер 4(11), С. 1532 - 1550

Опубликована: Ноя. 7, 2022

Abstract Animals must adapt their dietary choices to meet nutritional needs. How these needs are detected and translated into nutrient-specific appetites that drive food-choice behaviours is poorly understood. Here we show enteroendocrine cells of the adult female Drosophila midgut sense nutrients in response release neuropeptide F (NPF), which an ortholog mammalian Y-family gut-brain hormones. Gut-derived NPF acts on glucagon-like adipokinetic hormone (AKH) signalling induce sugar satiety increase consumption protein-rich food, adipose tissue promote storage ingested nutrients. Suppression NPF-mediated gut leads overconsumption while simultaneously decreasing intake yeast. Furthermore, gut-derived has a female-specific function promoting protein-containing food mated females. Together, our findings suggest NPF-to-AKH modulates specific regulates choice ensure homeostatic nutrients, providing insight hormonal mechanisms underlie hungers.

Язык: Английский

Процитировано

59
Endocrine cybernetics: neuropeptides as molecular switches in behavioural decisions DOI Creative Commons
Dick R. Nässel, Meet Zandawala

Open Biology, Год журнала: 2022, Номер 12(7)

Опубликована: Июль 1, 2022

Plasticity in animal behaviour relies on the ability to integrate external and internal cues from changing environment hence modulate activity synaptic circuits of brain. This context-dependent neuromodulation is largely based non-synaptic signalling with neuropeptides. Here, we describe select peptidergic systems Drosophila brain that act at different levels a hierarchy associated physiology. These regions, such as central complex mushroom bodies, which supervise specific behaviours. At top level there are small numbers large neurons arborize widely multiple areas orchestrate or global state manner. bottom local provide executive sensory gain intrinsically restricted parts neuronal circuits. The orchestrating receive interoceptive signals mediate energy sleep homeostasis, metabolic circadian timing, well affect food search, aggression mating. Some these can be triggers conflicting behaviours mating versus aggression, feeding, participate circuits, enabling choices switches.

Язык: Английский

Процитировано

55
Cellular and molecular organization of the Drosophila foregut DOI Creative Commons
Haolong Zhu, William B. Ludington, Allan C. Spradling

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(11)

Опубликована: Март 5, 2024

The animal foregut is the first tissue to encounter ingested food, bacteria, and viruses. We characterized adult Drosophila using transcriptomics better understand how it triages consumed items for digestion or immune response manages resources. Cell types were assigned validated GFP-tagged Gal4 reporter lines. Foregut-associated neuroendocrine cells play a major integrative role by coordinating gut activity with nutrition, microbiome, circadian cycles; some express clock genes. Multiple epithelial cell comprise proventriculus, central organ that secretes peritrophic matrix (PM) lining gut. Analyzing synthesizing individual PM layers revealed abundant mucin production close enterocytes, similar mammalian intestinal mucosa. esophagus salivary gland secreted proteins likely line esophageal surface, of which may generate commensal niche housing specific microbiome species. Overall, our results imply coordinates dietary sensing, hormonal regulation, immunity in manner has been conserved during evolution.

Язык: Английский

Процитировано

9
Towards early detection of neurodegenerative diseases: A gut feeling DOI Creative Commons

Stephanie Makdissi,

Brendon D. Parsons, Francesca Di Cara

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2023, Номер 11

Опубликована: Фев. 7, 2023

The gastrointestinal tract communicates with the nervous system through a bidirectional network of signaling pathways called gut-brain axis, which consists multiple connections, including enteric system, vagus nerve, immune endocrine signals, microbiota, and its metabolites. Alteration communications in axis is emerging as an overlooked cause neuroinflammation. Neuroinflammation common feature pathogenic mechanisms involved various neurodegenerative diseases (NDs) that are incurable debilitating conditions resulting progressive degeneration death neurons, such Alzheimer Parkinson diseases. NDs leading global disability, incidences expected to increase following decades if prevention strategies successful treatment remain elusive. To date, etiology unclear due complexity involving genetic environmental factors, diet microbiota. Emerging evidence suggests changes diet, alteration deregulation metabolism intestinal epithelium influence inflammatory status neurons linked disease insurgence progression. This review will describe players so-called diet-microbiota-gut-brain (DMGB) context NDs. We report recent findings from studies model organisms rodents fruit flies support role diets, commensals, epithelial functions primary regulator brain health. finish discussing pivotal metabolisms cellular organelles mitochondria peroxisomes maintaining DMGB how latter can be used early makers novel therapeutic targets.

Язык: Английский

Процитировано

14
Sex peptide receptor is not required for refractoriness to remating or induction of egg laying in Aedes aegypti DOI
I. Alexandra Amaro,

Margot Wohl,

Sylvie A. Pitcher

и другие.

Genetics, Год журнала: 2024, Номер 227(1)

Опубликована: Март 29, 2024

Across diverse insect taxa, the behavior and physiology of females dramatically changes after mating-processes largely triggered by transfer seminal proteins from their mates. In vinegar fly Drosophila melanogaster, protein sex peptide (SP) decreases likelihood female flies remating causes additional behavioral physiological that promote fertility including increasing egg production. Although SP is only found in genus, its receptor, receptor (SPR), widely conserved myoinhibitory (MIP) receptor. To test functional role SPR mediating postmating responses a non-Drosophila dipteran, we generated 2 independent Spr-knockout alleles yellow fever mosquito, Aedes aegypti. needed for cotton bollworm Helicoverpa armigera, Spr mutant Ae. aegypti show completely normal propensity increases laying. addition, injection synthetic or accessory gland homogenate D. melanogaster into virgin mosquitoes did not elicit these responses. Our results demonstrate required canonical aegypti, indicating other, as yet unknown, signaling pathways are likely responsible switches this disease vector.

Язык: Английский

Процитировано

5
The neuroendocrine and endocrine systems in insect – Historical perspective and overview DOI
Ian Orchard, Angela B. Lange

Molecular and Cellular Endocrinology, Год журнала: 2023, Номер 580, С. 112108 - 112108

Опубликована: Ноя. 11, 2023

Язык: Английский

Процитировано

11
Long-range neuropeptide relay as a central-peripheral communication mechanism for the context-dependent modulation of interval timing behaviors DOI Creative Commons

Tianmu Zhang,

Zekun Wu, Yutong Song

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 4, 2024

ABSTRACT Neuropeptides play crucial roles in regulating context-dependent behaviors, but the underlying mechanisms remain elusive. We investigate role of neuropeptide SIFa and its receptor SIFaR two distinct mating duration behaviors male Drosophila : Longer-Mating-Duration (LMD) Shorter-Mating-Duration (SMD). found that expression specific neurons is required for both LMD SMD behaviors. Social context sexual experience lead to synaptic reorganization between neurons, altering internal states brain. revealed SIFa-SIFaR/Crz-CrzR relay pathway essential generating interval timing with Crz being responsive activity neurons. Additionally, CrzR non-neuronal cells critical Our study provides insights into how neuropeptides their receptors modulate through plasticity calcium signaling, implications understanding neural circuitry neuropeptidergic system modulation behavioral adaptations.

Язык: Английский

Процитировано

4
What Drosophila can tell us about state-dependent peptidergic signaling in insects DOI Creative Commons
Dick R. Nässel

Insect Biochemistry and Molecular Biology, Год журнала: 2025, Номер unknown, С. 104275 - 104275

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Steroid hormone regulation of innate immunity in Drosophila melanogaster DOI Creative Commons
Scott A. Keith

PLoS Genetics, Год журнала: 2023, Номер 19(6), С. e1010782 - e1010782

Опубликована: Июнь 15, 2023

Endocrine signaling networks control diverse biological processes and life history traits across metazoans. In both invertebrate vertebrate taxa, steroid hormones regulate immune system function in response to intrinsic environmental stimuli, such as microbial infection. The mechanisms of this endocrine-immune regulation are complex constitute an ongoing research endeavor facilitated by genetically tractable animal models. 20-hydroxyecdysone (20E) is the major hormone arthropods, primarily studied for its essential role mediating developmental transitions metamorphosis; 20E also modulates innate immunity a variety insect taxa. This review provides overview our current understanding 20E-mediated responses. prevalence correlations between 20E-driven activation summarized range holometabolous insects. Subsequent discussion focuses on studies conducted using extensive genetic resources available Drosophila that have begun reveal underlying contexts development bacterial Lastly, I propose directions future into will advance knowledge how interactive endocrine coordinate animals' physiological responses microbes.

Язык: Английский

Процитировано

10
Gut-to-brain regulation of Drosophila aging through neuropeptide F, insulin, and juvenile hormone DOI Creative Commons
Jiangtian Chen, Marcela Nouzová, Fernando G. Noriega

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(43)

Опубликована: Окт. 16, 2024

Dietary restriction (DR) slows aging in many animals, while some cases, the sensory signals from diet alone are sufficient to retard or accelerate lifespan. The digestive tract is a candidate location sense nutrients, where neuropeptides secreted by enteroendocrine cells (EEC) produce systemic response food. Here, we measure how Drosophila neuropeptide F (NPF) into adult circulation EEC and find that specific differentially respond dietary sugar yeast. Female lifespan increased when gut NPF genetically depleted, this manipulation blunt longevity benefit conferred DR. Depletion of receptors at insulin-producing neurons brain also increases female lifespan, consistent with observations loss decreases neuronal insulin secretion. repressing reversed treating adults juvenile hormone (JH) analog. JH produced corpora allata, inhibition receptor tissue titer extends both males females, restored wild type Overall, modulate through interorgan communication mediated gut–brain–corpora allata axis, impacts its control titer. These data suggest consider human incretins their analogs, which used treat obesity diabetes, may impact aging.

Язык: Английский

Процитировано

3