bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Июнь 3, 2023
The
maintenance
of
epithelial
barrier
function
involves
cellular
tension,
with
cells
pulling
on
their
neighbors
to
maintain
integrity.
Wounding
interrupts
which
may
serve
as
an
early
signal
initiate
repair.
To
characterize
how
wounds
alter
we
used
a
laser-recoil
assay
map
cortical
tension
around
in
the
monolayer
Drosophila
pupal
notum.
Within
minute
wounding,
there
was
widespread
loss
along
both
radial
and
tangential
directions.
This
similar
levels
observed
Rok
inactivation.
Tension
subsequently
restored
wound,
first
distal
then
proximal
cells,
reaching
wound
margin
about
10
minutes
after
wounding.
Restoring
required
GPCR
Mthl10
IP3
receptor,
indicating
importance
this
calcium
signaling
pathway
known
be
activated
by
damage.
restoration
correlated
inward-moving
contractile
wave
that
has
been
previously
reported;
however,
itself
not
affected
knockdown.
These
results
indicate
transiently
increase
contract
absence
signaling,
but
is
critical
for
fully
resetting
baseline
it
disrupted
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Дек. 23, 2023
Basement
membranes
are
sheets
of
extracellular
matrix
separating
tissue
layers
and
providing
mechanical
support.
Their
properties
determined
largely
by
their
most
abundant
protein,
Collagen
IV
(Col4).
Although
basement
repaired
after
damage,
little
is
known
about
how.
To
wit,
since
membrane
it
unknown
how
damage
detected,
Col4
long-lived
regulated
to
avoid
fibrosis.
Using
the
adult
Journal of Visualized Experiments,
Год журнала:
2022,
Номер
182
Опубликована: Апрель 6, 2022
The
pupae
of
Drosophila
melanogaster
are
immobile
for
several
days
during
metamorphosis,
which
they
develop
a
new
body
with
thin
transparent
adult
integument.
Their
immobility
and
transparency
make
them
ideal
in
vivo
live
imaging
experiments.
Many
studies
have
focused
on
the
dorsal
epithelial
monolayer
pupal
notum
because
its
accessibility
relatively
large
size.
In
addition
to
mechanics
development,
has
been
an
tissue
study
wound
healing.
After
injury,
entire
repair
process
can
be
captured
by
over
6-12
h.
Despite
popularity
imaging,
very
few
published
utilized
fixed
samples.
Fixation
staining
common
approaches
nearly
all
other
tissues,
taking
advantage
repertoire
simple
cellular
stains
antibodies.
However,
is
fragile
prone
curling
distortion
after
removal
from
body,
making
it
challenging
complement
imaging.
This
protocol
offers
straightforward
method
fixing
notum,
both
intact
laser-wounding.
With
this
technique,
ventral
side
pupa
glued
down
coverslip
immobilize
pupa,
carefully
removed,
fixed,
stained.
epithelium
mounted
slide
or
between
two
coverslips
facilitate
tissue's
side.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Июнь 3, 2023
The
maintenance
of
epithelial
barrier
function
involves
cellular
tension,
with
cells
pulling
on
their
neighbors
to
maintain
integrity.
Wounding
interrupts
which
may
serve
as
an
early
signal
initiate
repair.
To
characterize
how
wounds
alter
we
used
a
laser-recoil
assay
map
cortical
tension
around
in
the
monolayer
Drosophila
pupal
notum.
Within
minute
wounding,
there
was
widespread
loss
along
both
radial
and
tangential
directions.
This
similar
levels
observed
Rok
inactivation.
Tension
subsequently
restored
wound,
first
distal
then
proximal
cells,
reaching
wound
margin
about
10
minutes
after
wounding.
Restoring
required
GPCR
Mthl10
IP3
receptor,
indicating
importance
this
calcium
signaling
pathway
known
be
activated
by
damage.
restoration
correlated
inward-moving
contractile
wave
that
has
been
previously
reported;
however,
itself
not
affected
knockdown.
These
results
indicate
transiently
increase
contract
absence
signaling,
but
is
critical
for
fully
resetting
baseline
it
disrupted
