Semaphorin 6A in Retinal Ganglion Cells Regulates Functional Specialization of the Inner Retina DOI Open Access
Rebecca E. James, Natalie R. Hamilton, L. Huffman

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 18, 2023

To form functional circuits, neurons must settle in their appropriate cellular locations and then project elaborate neurites to contact target synaptic neuropils. Laminar organization within the vertebrate retinal inner plexiform layer (IPL) facilitates pre- postsynaptic neurite targeting, yet, precise mechanisms underlying establishment of IPL subdomains are not well understood. Here we explore defining compartmentalization OFF ON generally, direction-selective specifically, developing IPL. We show that semaphorin 6A (Sema6A), a repulsive axon guidance cue, is required for delineation versus circuits IPL: Sema6a null IPL, boundary between domains blurred. Furthermore, Sema6A expressed by ganglion cells (RGCs) directs laminar segregation starburst amacrine cell (SAC) dendritic scaffolds, which themselves serve as substrate upon other elaborate. These results demonstrate first time RGCs, neuron-type born retina, play an active role specialization Retinal cell-dependent regulation scaffold prevents blurring murine layer.

Язык: Английский

Synaptic promiscuity in brain development DOI Creative Commons
Neele Wolterhoff, P. Robin Hiesinger

Current Biology, Год журнала: 2024, Номер 34(3), С. R102 - R116

Опубликована: Фев. 1, 2024

Precise synaptic connectivity is a prerequisite for the function of neural circuits, yet individual neurons, taken out their developmental context, readily form unspecific synapses. How does genome encode brain wiring in light this apparent contradiction? Synaptic specificity outcome long series processes and mechanisms before, during after synapse formation. much promiscuity permissible or necessary at moment partner choice depends on extent to which prior development restricts available partners subsequent corrects initially made can thereby play important roles precise connectivity, but also facilitate flexibility robustness. In review, we assess experimental evidence prevalence promiscuous formation development. Many well-established approaches are based genetic perturbation an assessment only adult; make it difficult pinpoint when given defect mechanism occurred. many cases, such studies reveal that restrict availability already Subsequently, choice, factors including competency, interaction dynamics molecular recognition further partners. The discussion through lens suggests algorithmic process neurons capable continuously prevented from making wrong choices, with no single time point sufficient explain outcome.

Язык: Английский

Процитировано

8
Retinal neurons establish mosaic patterning by excluding homotypic somata from their dendritic territories DOI Creative Commons
Christopher Kozlowski, Sarah E. Hadyniak, Jeremy N. Kay

и другие.

Cell Reports, Год журнала: 2024, Номер 43(8), С. 114615 - 114615

Опубликована: Авг. 1, 2024

In vertebrate retina, individual neurons of the same type are distributed regularly across tissue in a pattern known as mosaic. Establishment mosaics during development requires cell-cell repulsion among homotypic neurons, but mechanisms underlying this remain unknown. Here, we show that two mouse retinal cell types, OFF and ON starburst amacrine cells, establish mosaic spacing by using their dendritic arbors to repel neighboring somata. Using transgenic tools single-cell labeling, identify developmental period when somata contacted dendrites; these serve exclude from settling within neighbor's territory. Dendrite-soma exclusion is mediated MEGF10, cell-surface molecule required for patterning. Our results implicate dendrite-soma key mechanism raise possibility could be general patterning many types species.

Язык: Английский

Процитировано

3
Retinal ganglion cell-derived semaphorin 6A segregates starburst amacrine cell dendritic scaffolds to organize the inner retina DOI Creative Commons
Rebecca E. James, Natalie R. Hamilton, L. Huffman

и другие.

Development, Год журнала: 2024, Номер 151(22)

Опубликована: Ноя. 4, 2024

ABSTRACT To form functional circuits, neurons must settle in their appropriate cellular locations, and then project elaborate neurites to contact target synaptic neuropils. Laminar organization within the vertebrate retinal inner plexiform layer (IPL) facilitates pre- postsynaptic neurite targeting, yet precise mechanisms underlying establishment of IPL subdomains are not well understood. Here, we explore defining compartmentalization OFF ON generally, direction-selective specifically, developing mouse IPL. We show that semaphorin 6A (Sema6A), a repulsive axon guidance cue, is required for delineation versus circuits IPL: Sema6a null IPL, boundary between domains blurred. Furthermore, Sema6A expressed by ganglion cells (RGCs) directs laminar segregation starburst amacrine cell dendritic scaffolds, which themselves serve as substrate upon other elaborate. These results demonstrate RGCs, first type neuron born retina, play an active role specialization

Язык: Английский

Процитировано

1
Cellular and Molecular Mechanisms Underlying Synaptic Subcellular Specificity DOI Creative Commons
Mengqing Wang,

Jiale Fan,

Zhiyong Shao

и другие.

Brain Sciences, Год журнала: 2024, Номер 14(2), С. 155 - 155

Опубликована: Фев. 2, 2024

Chemical synapses are essential for neuronal information storage and relay. The synaptic signal received or sent from spatially distinct subcellular compartments often generates different outcomes due to the distance physical property difference. Therefore, final output of postsynaptic neurons is determined not only by type intensity inputs but also location. How specificity has long been focus study in neurodevelopment field. Genetic studies invertebrates such as Caenorhabditis elegans (C. elegans) have uncovered important molecular cellular mechanisms required specificity. Interestingly, similar were found mammalian cerebellum, hippocampus, cerebral cortex. This review summarizes comprehensive advances underlying specificity, focusing on C. rodents.

Язык: Английский

Процитировано

0
Vascular FLRT2 regulates venous-mediated angiogenic expansion and CNS barriergenesis DOI Open Access
C. Llaó-Cid,

Blanca Peguera,

Piotr Kobialka

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 27, 2024

Abstract Veins have emerged as the origin of all other endothelial cell subtypes needed to expand vascular networks during developmental and pathological neoangiogenesis. Here, we uncover significant role angioneurin Fibronectin Leucine Rich Transmembrane protein (FLRT) 2 in central nervous system (CNS) development mouse. Early postnatal FLRT2 deletion reveals specific defects retinal veins, impacting proliferation, sprouting polarity that result reduced tip cells at front. interacts with VE-cadherin together endocytic adaptor Numb contribute modulation adherens junction morphology both retina cerebral cortex vivo . Utilizing expansion microscopy, visualize altered dynamic distribution tissue mutants. Additionally, cortical vessels regulates crosstalk between tight junctions, influencing blood-brain barrier development. Our findings position a vein-specific crucial regulator CNS

Язык: Английский

Процитировано

0
Vascular FLRT2 regulates venous-mediated angiogenic expansion and CNS barriergenesis DOI Creative Commons
C. Llaó-Cid,

Blanca Peguera,

Piotr Kobialka

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Ноя. 29, 2024

Abstract Veins have emerged as the origin of all other endothelial cell subtypes needed to expand vascular networks during developmental and pathological neoangiogenesis. Here, we uncover role angioneurin Fibronectin Leucine Rich Transmembrane protein (FLRT) 2 in central nervous system (CNS) development mouse. Early postnatal FLRT2 deletion reveals specific defects retinal veins, impacting proliferation, sprouting polarity that result reduced tip cells at front. interacts with VE-cadherin together endocytic adaptor Numb contribute modulation adherens junction morphology both retina cerebral cortex vivo. Utilizing expansion microscopy, visualize altered dynamic distribution tissue mutants. Additionally, cortical vessels regulates crosstalk between tight junctions, influencing blood-brain barrier development. Our findings position a vein-specific regulator CNS

Язык: Английский

Процитировано

0
Semaphorin 6A in Retinal Ganglion Cells Regulates Functional Specialization of the Inner Retina DOI Open Access
Rebecca E. James, Natalie R. Hamilton, L. Huffman

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 18, 2023

To form functional circuits, neurons must settle in their appropriate cellular locations and then project elaborate neurites to contact target synaptic neuropils. Laminar organization within the vertebrate retinal inner plexiform layer (IPL) facilitates pre- postsynaptic neurite targeting, yet, precise mechanisms underlying establishment of IPL subdomains are not well understood. Here we explore defining compartmentalization OFF ON generally, direction-selective specifically, developing IPL. We show that semaphorin 6A (Sema6A), a repulsive axon guidance cue, is required for delineation versus circuits IPL: Sema6a null IPL, boundary between domains blurred. Furthermore, Sema6A expressed by ganglion cells (RGCs) directs laminar segregation starburst amacrine cell (SAC) dendritic scaffolds, which themselves serve as substrate upon other elaborate. These results demonstrate first time RGCs, neuron-type born retina, play an active role specialization Retinal cell-dependent regulation scaffold prevents blurring murine layer.

Язык: Английский

Процитировано

0