Ginseng Stem-and-Leaf Saponins Mitigate Chlorpyrifos-Evoked Intestinal Toxicity In Vivo and In Vitro: Oxidative Stress, Inflammatory Response and Apoptosis DOI Open Access
Silu Liu, Xiaoying Zhu, Hongyan Pei

и другие.

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(21), С. 15968 - 15968

Опубликована: Ноя. 4, 2023

In recent years, the phenomenon of acute poisoning and organ damage caused by organophosphorus pesticides (OPs) has been a frequent occurrence. Chlorpyrifos (CPF) is one most widely used pesticides. The main active components ginseng stems leaves are total stem-and-leaf saponins (GSLSs), which have various biological effects, including anti-inflammatory, antioxidant anti-tumor activities. We speculate that these could great potential in treatment severe diseases relief organophosphorus-pesticide-induced side effects; however, their mechanism action still unknown. At present, our work aims to evaluate effects GSLSs on antioxidation CPF vivo vitro pharmacological mechanisms. Mice treated with (5 mg/kg) showed intestinal mucosal injury, an elevated diamine oxidase (DAO) index, decreased expression occlusive protein-1 (ZO-1) protein, impaired oxidation system villi relaxation. addition, chlorpyrifos exposure significantly increased contents inflammatory factor TNF-α oxidative-stress-related indicators superoxide dismutase (SOD), catalase (CAT), glutathione SH (GSH), peroxidase (GSH-PX), reactive oxygen species (ROS) capacity (T-AOC); level lipid peroxide malondialdehyde (MDA); reversed Bax caspase; activated NF-κB-related proteins. Interestingly, GSLS supplementation at doses 100 200 mg/kg changes after treatment. Similar results were observed cultured RAW264.7 cells. Using flow cytometry, Hoechst staining (30 μg/mL, 60 μg/mL) improve cell injury apoptosis reduce accumulation ROS conclusion, play protective role against CPF-induced enterotoxicity inhibiting NF-κB-mediated alleviating oxidative stress inflammation.

Язык: Английский

Cadmium exposure caused cardiotoxicity in common carps (Cyprinus carpio L.): miR-9-5p, oxidative stress, energetic impairment, mitochondrial division/fusion imbalance, inflammation, and autophagy DOI
Yuhao Liu, Lin Xu, Zhiyu Hao

и другие.

Fish & Shellfish Immunology, Год журнала: 2023, Номер 138, С. 108853 - 108853

Опубликована: Май 27, 2023

Язык: Английский

Процитировано

50
Luteolin enhanced antioxidant capability and induced pyroptosis through NF-κB/NLRP3/Caspase-1 in splenic lymphocytes exposure to ammonia DOI
Dechun Chen, F Shen, Jiahao Liu

и другие.

The Science of The Total Environment, Год журнала: 2024, Номер 919, С. 170699 - 170699

Опубликована: Фев. 5, 2024

Язык: Английский

Процитировано

24
Se Alleviated Pb-Caused Neurotoxicity in Chickens: SPS2-GPx1-GSH-IL-2/IL-17-NO Pathway, Selenoprotein Suppression, Oxidative Stress, and Inflammatory Injury DOI Creative Commons
Yansheng Li,

Jiatian Liang,

Chunyu Jiang

и другие.

Antioxidants, Год журнала: 2024, Номер 13(3), С. 370 - 370

Опубликована: Март 18, 2024

Lead (Pb), a heavy metal environmental pollutant, poses threat to the health of humans and birds. Inflammation is one most common pathological phenomena in case illness poisoning. However, underlying mechanisms inflammation remain unclear. The cerebellum thalamus are important parts nervous system. To date, there have been no reports Pb inducing animal cerebellums or thalami. Selenium (Se) can relieve Therefore, we aimed explore mechanism by which Se alleviates toxicity thalami chickens establishing chicken or/and treatment model. Our results demonstrated that exposure caused inflammatory damage thalami, evidenced characteristics inflammation, decrease anti-inflammatory factors (interleukin (IL)-2 interferon-γ (INF-γ)), increase pro-inflammatory (IL-4, IL-6, IL-12β, IL-17, nitric oxide (NO)). Moreover, found IL-2/IL-17–NO pathway took part Pb-caused injury. above findings were reversed supplementation dietary Se, meaning relieved via pathway. In addition, an up-regulated oxidative index malondialdehyde (MDA) two down-regulated antioxidant indices (glutathione (GSH) total capacity (TAC)) recorded after received stimulation, indicating excess oxidant/antioxidant imbalance stress, stress mediated GSH–IL-2 axis. Interestingly, inhibited four glutathione peroxidase (GPx) family members (GPx1, GPx2, GPx3, GPx4), three deiodinase (Dio) (Dio1, Dio2, Dio3), fifteen other selenoproteins (selenophosphate synthetase 2 (SPS2), selenoprotein (Sel)H, SelI, SelK, SelM, SelO, SelP1, SelPb, SelS, SelT, SelU, (Sep)n1, Sepw1, Sepx1, Sep15), suggesting reduced resulted involving SPS2–GPx1–GSH supplementation, as expected, changes mentioned above, improved mitigated chickens. For first time, discovered SPS2–GPx1–GSH–IL-2/IL-17–NO involved complex conclusion, this study Pb-induced This offers novel insights into pollutant-caused poisoning provides theoretical basis for detoxification effect against toxic pollutants.

Язык: Английский

Процитировано

23
Molecular mechanism of apoptosis induced by 4-tBP in common carp (Cyprinus carpio L.) head kidneys was explored from various angles: Hippo pathway, miR-203a, oxidative stress, ER stress, and mitochondrial pathway DOI

Jiatian Liang,

Lin Xu, Chunyu Jiang

и другие.

Aquaculture, Год журнала: 2024, Номер 589, С. 740981 - 740981

Опубликована: Апрель 21, 2024

Язык: Английский

Процитировано

23
Acute exposure to polystyrene nanoplastics induced oxidative stress in Sepia esculenta Larvae DOI Creative Commons
Xiumei Liu, Zan Li, Qian Li

и другие.

Aquaculture Reports, Год журнала: 2024, Номер 35, С. 102004 - 102004

Опубликована: Фев. 28, 2024

Nanoplastics (NPs, < 1 μm) have attracted widespread attention due to their potential threats organisms, ecosystem, and public health. However, there is limited research on the effects of NPs cephalopods. Sepia esculenta (Mollusks, Cephalopoda, Sepioidea), an economically major species Sepiidea family in world, well received by consumers for its delicious quality rich nutrition. In present study, S. larvae were treated with polystyrene nanoplastics (PS-NPs) at 50 μg/mL 4 h, then physiological biomarkers (SOD, GSTs MDA) RNA-Seq whole body performed. Research results suggested that PS-NPs exposure had induced oxidative stress larvae. addition, several key genes signaling pathways related obtained based DEGs, GO KEGG enrichment analyses, as PPI construction analysis transcriptome data. This study enriches different organisms provides insights references revealing mechanisms caused molecular level.

Язык: Английский

Процитировано

22
The investigation on stress mechanisms of Sepia esculenta larvae in the context of global warming and ocean acidification DOI Creative Commons
Yongjie Wang, Xiumei Liu,

Weijun Wang

и другие.

Aquaculture Reports, Год журнала: 2024, Номер 36, С. 102120 - 102120

Опубликована: Май 8, 2024

Sepia esculenta is a tasty and nutritious cephalopod. But the trend toward global warming accelerating with massive emissions of greenhouse gases. An increase in seawater temperature decrease pH are great challenge for artificial breeding culture S. esculenta. In this study, we investigated contingency mechanism larvae under both high low conditions by transcriptome analysis. A total 1235 DEGs were identified. functional enrichment analysis results suggest that led to malignant proliferation exceptional cells larvae. comprehensive KEGG signaling pathway protein-protein interaction network (PPI) identified sixteen key genes conditions. The three highest quantities interactions or involved more pathways as hub affecting normal physiological processes effects ocean acidification on metabolism immunity preliminarily provide necessary help captive face climate change.

Язык: Английский

Процитировано

19
Molecular mechanism of selenium against lead-induced apoptosis in chicken brainstem relating to heat shock protein, selenoproteins, and inflammatory cytokines DOI Creative Commons
Dechun Chen, Weikang Yu, Zhiyu Hao

и другие.

Ecotoxicology and Environmental Safety, Год журнала: 2024, Номер 272, С. 116028 - 116028

Опубликована: Фев. 3, 2024

Extensive application of lead (Pb) brought about environmental pollution and toxic reactions organisms. Selenium (Se) has the effect antagonizing Pb poisoning in humans animals. However, it is still unclear how causes brainstem toxicity. In present study, we wanted to investigate whether Se can alleviate toxicity chicken brainstems by reducing apoptosis. One hundred eighty chickens were randomly divided into four groups, namely control group, Se/Pb group. Morphological examination, ultrastructural observation, relative mRNA expressions genes on heat shock proteins (HSPs); selenoproteins; inflammatory cytokines; apoptosis-related factors investigated. The results showed that exposure led tissue damage apoptosis brainstems. Furthermore, an atypical expression HSPs (HSP27, HSP40, HSP60, HSP70, HSP90); selenoprotein family glutathione peroxidase (GPx) 1, GPx2, GPx3, GPx4), thioredoxin reductases (Txnrd) (Txnrd1, Txnrd2, Txnrd3), dio famliy (diodothyronine deiodinases (Dio)1, Dio2, Dio3), as well other selenoproteins (selenoprotein (Sel)T, SelK, SelS, SelH, SelM, SelU, SelI, SelO, Selpb, n1 (Sepn1), Sepp1, Sepx1, Sepw1, 15-kDa (Sep15), selenophosphate synthetases 2 (SPS2)); cytokines (Interleukin (IL-2), IL-4, IL-6, IL-12β, IL-17, Interferon-γ (IFN-γ)); (B-cell lymphoma-2 (Bcl-2), tumor protein 53 (p53), Bcl-2 Associated X (Bax), Cytochrome c (Cyt c), Caspase-3) identified. An reaction induced after Pb. alleviated abnormal HSPs, selenoproteins, cytokines, tissues treated with indicated inflammatory, involved Se-resisted poisoning. Overall, had resistance against poisoning, be act antidote for

Язык: Английский

Процитировано

18
The impact of dietary supplementation of polysaccharide derived from Polygonatum sibiricum on growth, antioxidant capacity, meat quality, digestive physiology, and gut microbiota in broiler chickens DOI Creative Commons
Bing Yang, Xiaofeng Li, Noura M. Mesalam

и другие.

Poultry Science, Год журнала: 2024, Номер 103(6), С. 103675 - 103675

Опубликована: Март 22, 2024

Polygonatum sibiricum polysaccharide (PSP) has demonstrated diverse medicinal properties, extensively researched for human applications. Nonetheless, there is a lack of studies investigating the potential advantages PSP in poultry farming. The present study investigated impact incorporating into broiler diets on their growth performance, meat quality, blood metabolites, antioxidative status, and ileal histomorphology. Two hundred forty-one-day-old male Ross-308 chicks (44.98 ± 0.79 g) were randomly assigned to 3 experimental groups, with 8 replicates 10 birds each. fed supplemented at 0, 400, 800 mg/kg (control, PSP400, PSP800, respectively). results revealed linear (P > 0.05) improvement body weight gain, European production efficiency index, feed conversion ratio during grower (22-35 d) overall periods (1-35 d). pH levels ingluvies, ileum, cecum exhibited reduction PSP800 group d 21 35, respectively. Villus height crypt depth increased PSP400 groups compared control group. decreased hydrogen peroxide (H

Язык: Английский

Процитировано

11
Chlorantraniliprole induces mitophagy, ferroptosis, and cytokine homeostasis imbalance in grass carp (Ctenopharyngodon idella) hepatocytes via the mtROS-mitochondrial fission/fusion axis DOI

Bendong Shi,

Qiaohan Liu,

Chenchen Xu

и другие.

Pesticide Biochemistry and Physiology, Год журнала: 2024, Номер 200, С. 105830 - 105830

Опубликована: Фев. 13, 2024

Язык: Английский

Процитировано

9
Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers DOI

Gaolong Zhong,

Yuanxu Li, Lei Li

и другие.

Journal of Hazardous Materials, Год журнала: 2023, Номер 458, С. 131908 - 131908

Опубликована: Июнь 21, 2023

Язык: Английский

Процитировано

19