Luteolin enhanced antioxidant capability and induced pyroptosis through NF-κB/NLRP3/Caspase-1 in splenic lymphocytes exposure to ammonia

The Science of The Total Environment, Год журнала: 2024, Номер 919, С. 170699 - 170699

Опубликована: Фев. 5, 2024

Язык: Английский

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The Role of Cannabidiol in Liver Disease: A Systemic Review

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(4), С. 2370 - 2370

Опубликована: Фев. 17, 2024

Cannabidiol (CBD), a non-psychoactive phytocannabinoid abundant in Cannabis sativa, has gained considerable attention for its anti-inflammatory, antioxidant, analgesic, and neuroprotective properties. It exhibits the potential to prevent or slow progression of various diseases, ranging from malignant tumors viral infections neurodegenerative disorders ischemic diseases. Metabolic dysfunction-associated steatotic liver disease (MASLD), formerly known as non-alcoholic fatty (NAFLD), alcoholic disease, hepatitis stand prominent causes morbidity mortality chronic diseases globally. The literature substantiated CBD’s therapeutic effects across diverse vivo vitro models. However, precise mechanism action remains elusive, an absence evidence hinders translation into clinical practice. This comprehensive review emphasizes wealth data linking CBD Importantly, we delve detailed discussion receptors through which might exert effects, including cannabinoid receptors, CB1 CB2, peroxisome proliferator-activated (PPARs), G protein-coupled receptor 55 (GPR55), transient channels (TRPs), their intricate connections with In conclusion, address new questions that warrant further investigation this evolving field.

Язык: Английский

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Mogroside V alleviates inflammation response by modulating miR-21-5P/SPRY1 axis

Food & Function, Год журнала: 2024, Номер 15(4), С. 1909 - 1922

Опубликована: Янв. 1, 2024

Mogroside V (MV) is a natural sweetener extracted from the edible plant Siraitia grosvenorii that possesses anti-inflammatory bioactivity. It has been reported microRNAs (miRNAs) play an important role in inflammation response suppression by agents. However, whether anti-inflammation effect of mogroside related to miRNAs and underlying mechanism remains unclear. Our study aimed identify key for MV reveal its mechanisms. results showed effectively alleviated lung ovalbumin-induced (OVA-induced) asthmatic mice. miRNA-seq mRNA-seq combined analysis identified miR-21-5p as miRNA inhibition it predicted SPRY1 be target gene miR-21-5p. We found significantly inhibited production tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-2 (IL-2), interleukin-6 (IL-6), nitric oxide (NO), well protein expression p-P65/P65, cyclooxygenase-2 (COX-2), inducible synthase (iNOS) OVA-induced mice LPS-treated RAW 264.7 cells. Moreover, release ROS increased LPS-stimulated cells but was mitigated pretreatment. In meantime, decreased MV, leading increase Furthermore, overexpression or knockdown reversed MV's protective on inflammatory responses. Conversely, enhanced responses LPS-exposed Therefore, significant downregulation upregulation vitro vivo, MiR-21-5p/SPRY1 may novel therapeutic targets treatment.

Язык: Английский

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New insights into Microalgal astaxanthin's effect on Lambda-cyhalothrin-induced lymphocytes immunotoxicity in Cyprinus carpio: Involving miRNA-194-5p-FoxO1-mediated-mitophagy and pyroptosis

Fish & Shellfish Immunology, Год журнала: 2023, Номер 141, С. 109046 - 109046

Опубликована: Сен. 1, 2023

Язык: Английский

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Cypermethrin induces apoptosis, autophagy and inflammation via ERS-ROS-NF-κB axis in hepatocytes of carp (Cyprinus carpio)

Pesticide Biochemistry and Physiology, Год журнала: 2023, Номер 196, С. 105625 - 105625

Опубликована: Сен. 22, 2023

Язык: Английский

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STC1 competitively binding βPIX enhances melanoma progression via YAP nuclear translocation and M2 macrophage recruitment through the YAP/CCL2/VEGFA/AKT feedback loop

Pharmacological Research, Год журнала: 2024, Номер 204, С. 107218 - 107218

Опубликована: Май 18, 2024

This study investigates the role of Stanniocalcin-1 (STC1) in melanoma progression, with a focus on its impact metastasis, angiogenesis, and immune evasion. Systematic bioinformatics analysis revealed potential influence STC1 dysregulation prognosis, cell infiltration, response to therapy, cellular functions. In vitro assays were conducted assess proliferation, invasion, migration, angiogenesis capabilities A375 cells. vivo experiments utilizing C57BL/6 J mice established lung metastasis model using B16-F10 cells evaluate macrophage infiltration M2 polarization. A Transwell co-culture system was employed explore crosstalk between macrophages. Molecular interactions among STC1, YAP, βPIX, CCL2 are investigated mass spectrometry, Co-Immunoprecipitation, Dual-Luciferase Reporter Assay, Chromatin Immunoprecipitation experiments. found enhance by promoting recruitment polarization macrophages, thereby fostering an immunosuppressive microenvironment. Mechanistically, competes YAP for binding βPIX within KER domain cells, leading activation subsequent upregulation. CCL2-induced macrophages secrete VEGFA, which enhances tumor vascularization increases expression via AKT signaling pathway establishing pro-metastatic feedback loop. Notably, STC1-induced PD-L1 expression, Silencing efficacy PD-1 checkpoint therapy mice. research elucidates STC1's complex tumor-associated proposing as therapeutic target countering augmenting immunotherapy.

Язык: Английский

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Nootkatone Mitigated Melamine-Evoked Hepatotoxicity by Featuring Oxidative Stress and Inflammation Interconnected Mechanisms: In Vivo and In Silico Approaches

Toxics, Год журнала: 2023, Номер 11(9), С. 784 - 784

Опубликована: Сен. 15, 2023

Melamine (ML) is a common environmental contaminant, commonly used in food fraud, representing serious health hazard and jeopardizing human animal health. Recently, nootkatone (NK), naturally occurring sesquiterpenoid, has garnered considerable attention due to its potential therapeutic advantages. We investigated the mechanisms underlying protective effects of NK against ML-induced liver injury rats. Five groups were utilized: control, ML, NK10, ML-NK5, ML-NK10. ML induced substantial hepatotoxicity, including alterations biochemical parameters histology. The oxidative distress triggered by increased generation malondialdehyde (MDA) nitric oxide (NO) decreased levels reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) activities. In addition, expression nuclear factor-erythroid 2-related factor 2 (Nrf2) kappa beta (NF-κB) observed hepatocytes, which indicated occurrence inflammatory changes following exposure. These alleviated supplementation dose-dependent manner. data revealed that favorable attributed, at least part, antioxidant anti-inflammatory properties. Moreover, our results supported molecular docking studies good fit interactions between enzymes. Thus, current study demonstrated new additive for prevention or treatment toxicity.

Язык: Английский

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Effects of Perfluorooctane Sulfonic Acid Exposure on Intestinal Microbial Community, Lipid Metabolism, and Liver Lesions in Mice

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2648 - 2648

Опубликована: Март 14, 2025

Perfluorooctane sulfonic acid (PFOS) is a persistent organic pollutant that has attracted much attention due to its wide environmental distribution and potential toxicity. Intestinal microbiota an important regulator of host health, composition metabolic function are easily interfered with by pollutants. In this study, the effects PFOS exposure on gut microbiota, lipid metabolism, health were investigated in mice. The results showed did not significantly change α diversity, but affected β diversity community structure intestinal microflora At taxonomic level, ratio Firmicutes Bacteroidetes decreased, changes abundance specific bacteria closely related liver diseases metabolism disorders. also gut–liver axis mechanism, increased blood lipids indicators mice, induced histological lesions. This study revealed toxic mechanism mediated providing new research perspective for problems caused pollutants theoretical support formulation relevant public policies.

Язык: Английский

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Mechanistic Insights Into the Role of Selenoprotein M in Nickel-Induced Lung Fibrosis

Biological Trace Element Research, Год журнала: 2025, Номер unknown

Опубликована: Апрель 28, 2025

Язык: Английский

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Hepatotoxicity of the Pesticide Avermectin Exposure to Freshwater-Farmed Carp: Evidence from In Vivo and In Vitro Research

Journal of Agricultural and Food Chemistry, Год журнала: 2023, Номер 71(51), С. 20654 - 20670

Опубликована: Дек. 13, 2023

Avermectin (AVM) is presently one of the most extensively employed insecticides across globe. A number toxicity research studies AVM have been carried out in freshwater-farmed carp; however, there are currently no on liver. This investigation aims to replicate an acute liver injury model induced by carp, subsequently analyzing adverse effects imposed nontarget species while delving into potential mechanisms underlying its toxicity. In this study, we found that AVM-exposed carp tissue showed cellular hydration degeneration and necrosis reduced viability hepatocyte L8824. Second, oxidative stress stimulation led reactive oxygen (ROS) accumulation Ca2+ overload L8824, suggesting exposure induces mitochondrial dysfunction hepatocytes. inflammation inducing kinetic disruption, which triggered hepatic injury. autophagy apoptosis ROS mediated AVM-induced apoptosis. The formation attenuated conclusion, present study elucidated hepatotoxicity freshwater aquaculture exposed pesticide AVM, emphasized importance monitoring contamination aquatic environments, provided theoretical references for targeted prevention carp.

Язык: Английский

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