Heliyon,
Год журнала:
2024,
Номер
11(1), С. e41535 - e41535
Опубликована: Дек. 27, 2024
Haemophilus
influenzae
(H.
influenzae,
Hi)
is
an
opportunistic
bacterium
that
colonizes
the
upper
respiratory
tract
of
humans
and
frequently
causes
meningitis,
pneumonia,
sepsis,
other
severe
infections
in
children.
Early
accurate
detection
H.
essential
for
effective
diagnosis
treatment.
In
this
study,
we
established
a
novel
diagnostic
method
by
integrating
CRISPR-Cas12a
platform
with
multiple
cross-displacement
amplification
(MCDA),
termed
Hi-MCDA-CRISPR
assay.
This
offers
efficient
highly
precise
tool
identification
influenzae.
system,
outer
membrane
protein
(OMP)
P6
was
pre-amplified
using
MCDA
The
CRISPR-Cas12a-gRNA
complex
specifically
recognized
bound
to
amplified
gene,
forming
ternary
triggered
nonspecific
trans-cleavage
Cas12a
effector,
which
subsequently
degraded
fluorescent-quenched
single-stranded
DNA
(ssDNA)
probes,
resulting
emission
detectable
fluorescent
signals.
After
optimizing
reaction
conditions,
assay
proved
capable
completing
within
45
min,
including
40
min
pre-amplification
at
62
°C
5
cleavage
37
°C.
able
detect
genomic
concentrations
as
low
50
fg
showed
no
cross-reactivity
non-H.
pathogens.
Furthermore,
successfully
analyzed
65
clinical
sputum
samples.
These
findings
suggest
promising
new
rapid
reliable
infection.
Critical Reviews in Analytical Chemistry,
Год журнала:
2024,
Номер
unknown, С. 1 - 25
Опубликована: Сен. 17, 2024
This
comprehensive
review
delves
into
the
advancements
and
challenges
in
biosensing,
with
a
strong
emphasis
on
transformative
potential
of
CRISPR
technology
for
early
rapid
detection
infectious
diseases.
It
underscores
versatility
CRISPR/Cas
systems,
highlighting
their
ability
to
detect
both
nucleic
acids
non-nucleic
acid
targets,
seamless
integration
isothermal
amplification
techniques.
The
provides
thorough
examination
latest
developments
CRISPR-based
biosensors,
detailing
unique
properties
such
as
high
specificity
programmability,
which
make
them
particularly
effective
detecting
disease-associated
acids.
While
focuses
due
its
critical
role
diagnosing
diseases,
it
also
explores
broader
applications
thereby
acknowledging
technology's
potential.
Additionally,
identifies
existing
challenges,
need
improved
signal
real-world
applicability,
offers
future
perspectives
aimed
at
overcoming
these
hurdles.
ultimate
goal
is
advance
development
highly
sensitive
specific
biosensors
that
can
be
used
widely
improving
human
health,
point-of-care
settings
resource-limited
environments.
Biosensors,
Год журнала:
2025,
Номер
15(1), С. 44 - 44
Опубликована: Янв. 13, 2025
Plastic
pollution,
particularly
from
microplastics
(MPs)
and
nanoplastics
(NPs),
has
become
a
critical
environmental
health
concern
due
to
their
widespread
distribution,
persistence,
potential
toxicity.
MPs
NPs
originate
primary
sources,
such
as
cosmetic
microspheres
or
synthetic
fibers,
secondary
fragmentation
of
larger
plastics
through
degradation.
These
particles,
typically
less
than
5
mm,
are
found
globally,
deep
seabeds
human
tissues,
known
adsorb
release
harmful
pollutants,
exacerbating
ecological
risks.
Effective
detection
quantification
essential
for
understanding
mitigating
impacts.
Current
analytical
methods
include
physical
chemical
techniques.
Physical
methods,
optical
electron
microscopy,
provide
morphological
details
but
often
lack
specificity
time-intensive.
Chemical
analyses,
Fourier
transform
infrared
(FTIR)
Raman
spectroscopy,
offer
molecular
face
challenges
with
smaller
particle
sizes
complex
matrices.
Thermal
including
pyrolysis
gas
chromatography–mass
spectrometry
(Py-GC-MS),
compositional
insights
destructive
limited
in
analysis.
Emerging
(bio)sensing
technologies
show
promise
addressing
these
challenges.
Electrochemical
biosensors
cost-effective,
portable,
sensitive
platforms,
leveraging
principles
voltammetry
impedance
detect
adsorbed
pollutants.
Plasmonic
techniques,
surface
plasmon
resonance
(SPR)
surface-enhanced
spectroscopy
(SERS),
high
sensitivity
nanostructure-enhanced
detection.
Fluorescent
utilizing
microbial
enzymatic
elements
enable
the
real-time
monitoring
plastic
degradation
products,
terephthalic
acid
polyethylene
terephthalate
(PET).
Advancements
innovative
approaches
pave
way
more
accurate,
scalable,
environmentally
compatible
solutions,
contributing
improved
remediation
strategies.
This
review
highlights
advanced
section
on
prospects
that
address
could
lead
significant
advancements
monitoring,
highlighting
necessity
testing
new
sensing
developments
under
real
conditions
(composition/matrix
samples),
which
overlooked,
well
study
peptides
novel
recognition
element
microplastic
sensing.
ABSTRACT
A
novel
fluorescence‐based
sensor
has
been
developed
for
the
sensitive
detection
of
malathion,
an
organophosphorus
pesticide,
using
sulfur‐doped
quantum
dots
(SQDs)
embedded
within
graphitic
carbon
nitride
(g‐C₃N₄)
nanosheets.
The
SQDs
were
synthesized
through
a
hydrothermal
method,
whereas
g‐C₃N₄
nanosheets
produced
via
exfoliation
process.
resulting
SQDs@g‐C₃N₄
nanocomposite
demonstrated
outstanding
performance
malathion
in
food
samples,
exhibiting
wide
linear
range
10–120
μM
and
exceptionally
low
limit
0.02
μM.
This
sensitivity
allows
accurate
rapid
pesticide
monitoring
at
trace
levels.
sensor's
was
optimized
by
varying
experimental
conditions,
ensuring
that
it
provided
high
sensitivity,
excellent
stability,
impressive
selectivity
toward
even
complex
matrices.
Biosensors,
Год журнала:
2025,
Номер
15(3), С. 155 - 155
Опубликована: Март 2, 2025
High-sensitivity
and
high-specificity
biodetection
is
critical
for
advancing
applications
in
life
sciences,
biosafety,
food
safety,
environmental
monitoring.
CRISPR/Cas
systems
have
emerged
as
transformative
tools
biosensing
due
to
their
unparalleled
specificity,
programmability,
unique
enzymatic
activities.
They
exhibit
two
key
cleavage
behaviors:
precise
ON-target
guided
by
specific
protospacers,
which
ensures
accurate
target
recognition,
bystander
activity
triggered
upon
binding,
enables
robust
signal
amplification.
These
properties
make
highly
versatile
designing
biosensors
ultra-sensitive
detection.
This
review
comprehensively
explores
recent
advancements
system-based
biosensors,
highlighting
impact
on
improving
performance.
We
discuss
the
integration
of
with
diverse
readout
mechanisms,
including
electrochemical,
fluorescent,
colorimetric,
surface-enhanced
Raman
scattering
(SERS),
so
on.
Additionally,
we
examine
development
integrated
systems,
such
microfluidic
devices
portable
leverage
technology
point-of-care
testing
(POCT)
high-throughput
analysis.
Furthermore,
identify
unresolved
challenges,
aiming
inspire
innovative
solutions
accelerate
translation
these
technologies
into
practical
diagnostics,
food,
environment
safety.
Pharmacogenetics and Pharmacogenomics,
Год журнала:
2025,
Номер
2, С. 29 - 36
Опубликована: Март 4, 2025
Introduction.
CRISPR
(Clustered
Regularly
Interspaced
Short
Palindromic
Repeats)
has
been
recognized
as
a
revolutionary
advancement
in
the
biomedical
field,
offering
unparalleled
precision
and
versatility
genome
editing.
This
review
examines
transformative
potential
of
diagnostic
therapeutic
tool
for
various
diseases.
Methods.
A
systematic
was
conducted
following
PRISMA
(Preferred
Reporting
Items
Systematic
Reviews
Meta-Analyses)
guidelines.
comprehensive
search
PubMed,
Scopus,
Google
Scholar,
Web
Science
performed
to
identify
relevant
Studies
published
between
January
2015
2025.
The
inclusion
criteria
focused
on
peer-reviewed
articles
discussing
CRISPR-based
diagnostics,
applications,
technological
advancements.
were
screened,
assessed
quality
using
CASP
framework,
categorized
into
thematic
areas
analysis.
Results.
platforms,
such
SHERLOCK
DETECTR,
analyzed
their
sensitivity
rapidity
detecting
pathogens,
cancer
biomarkers,
genetic
mutations.
Emerging
innovations,
including
prime
base
editing,
have
explored
role
expanding
capabilities
CRISPR.
Additionally,
advancements
delivery
mechanisms
use
alternative
Cas
proteins
discussed
impact
clinical
applicability.
Conclusions.
Ethical,
regulatory,
accessibility
challenges
associated
with
technology
are
highlighted,
emphasizing
importance
responsible
development
equitable
deployment.
connects
cutting-edge
translational
underscores
significant
shaping
future
medicine
global
health.
Journal of Preventive Diagnostic and Treatment Strategies in Medicine,
Год журнала:
2025,
Номер
4(1), С. 1 - 8
Опубликована: Янв. 1, 2025
Abstract
BACKGROUND:
The
CRISPR-Cas
system
(Clustered
Regularly
Interspaced
Short
Palindromic
Repeats
and
CRISPR-associated
proteins)
serves
as
an
adaptive
immune
defense
mechanism
in
Klebsiella
pneumoniae
.
aim
of
the
study
was
to
evaluate
correlation
between
presence
antimicrobial
resistance
profiles
K.
isolates.
METHODS:
This
included
patients
all
ages
genders,
with
histories
recorded
(name,
age,
gender,
sample
type
blood,
sputum,
urine)
conducted
at
Al-Hilla
Teaching
Hospitals,
Babylon
City
(February–August
2024);
200
clinical
samples
were
cultured
on
various
media
identified
through
biochemical
tests
VITEK®2.
Antibiotic
susceptibility
assessed
using
Kirby–Bauer
method
per
CLSI
2024
guidelines.
Tested
antibiotics
imipenem,
augmentin,
amikacin,
tetracycline,
ceftriaxone,
gentamicin,
chloramphenicol,
cefotaxime,
ceftazidime,
colistin.
DNA
extracted
a
Geneaid
kit,
polymerase
chain
reaction
detected
Cas1A
Cas1B
genes
specific
primers.
Statistical
analyses
performed
IBM
SPSS
Statistics,
version
25.0
(IBM
Corp.,
Armonk,
NY,
USA).
Data
presented
form
tables,
charts,
graphs.
A
P
<
0.05
considered
statistically
significant.
Categorical
variables
compared
t
-tests,
while
Pearson’s
coefficient
(
r
)
used
assess
associations.
RESULTS:
present
analyzed
(blood,
from
Al-Hillah
Hospital,
identifying
12.5%
cases,
aligning
previous
studies.
Molecular
detection
confirmed
systems,
Cas1
68%
B
60%
testing
revealed
multidrug
resistance,
though
isolates
Type
I-E
showed
higher
such
aztreonam,
imipenem
≤
0.01).
appears
limit
horizontal
gene
transfer
but
does
not
fully
mitigate
intrinsic
underscoring
its
complex
role
management.
CONCLUSIONS:
highlights
systems’
,
isolates,
respectively.
increased
suggesting
potential
modulation
