Chronic Vitamin E Deficiency Dysregulates Purine, Phospholipid, and Amino Acid Metabolism in Aging Zebrafish Skeletal Muscle

Antioxidants, Год журнала: 2023, Номер 12(6), С. 1160 - 1160

Опубликована: Май 26, 2023

Muscle wasting occurs with aging and may be a result of oxidative stress damage potentially inadequate protection by lipophilic antioxidants, such as vitamin E. Previous studies have shown muscular abnormalities behavioral defects in E-deficient adult zebrafish. To test the hypothesis that there is an interaction between muscle degeneration caused E deficiency, we evaluated long-term deficiency skeletal zebrafish using metabolomics. Zebrafish (55 days old) were fed E+ E− diets for 12 or 18 months. Then, samples analyzed UPLC-MS/MS. Data to highlight metabolite pathway changes seen either status both. We found altered purines, various amino acids, DHA-containing phospholipids. Vitamin at months was associated acid metabolism, specifically tryptophan pathways, systemic regulation purine In sum, while both induced did some overlap dysregulated metabolic each factor also presented unique alterations, which require further study more confirmatory approaches.

Язык: Английский

---

Heat exposure promotes sarcopenia via gut microbiota‐derived metabolites

Aging Cell, Год журнала: 2024, Номер unknown

Опубликована: Окт. 29, 2024

Abstract The unprecedented rise in global ambient temperatures the last decade has significantly impacted human health, yet how heat exposure affects development of sarcopenia remains enigmatic. Here, we demonstrate that chronic induces skeletal muscle volume loss, leading to strength and functional decline mice. microbiota composition heat‐exposed mice was analyzed using 16S ribosomal DNA analysis. Liquid chromatography‐mass spectrometry (LC–MS) used explore effects on blood metabolome further analyze correlation between metabolism gut microbiota. Transplantation from germ‐free sufficient increase adverse function host. Mechanistically, an untargeted metabolomics strategy, reveal altered due high is associated with elevated serum levels homocitrulline. Homocitrulline causes mitochondrial dysfunction myocytes by exacerbating ferroptosis levels. And Nrf2 activator (Oltipraz) supplementation alleviates atrophy induced exposure. Our findings detrimental provide new strategies for treating sarcopenia.

Язык: Английский

---

The causal relationship of human blood metabolites with the components of Sarcopenia: a two-sample Mendelian randomization analysis

BMC Geriatrics, Год журнала: 2024, Номер 24(1)

Опубликована: Апрель 15, 2024

Abstract Background Sarcopenia is a progressive loss of muscle mass and function. Since skeletal plays critical role in metabolic homeostasis, identifying the relationship blood metabolites with sarcopenia components would help understand etiology sarcopenia. Methods A two-sample Mendelian randomization study was conducted to examine causal Summary genetic association data for 309 known were obtained from Twins UK cohort KORA F4 (7824 participants). The summary statistics [hand grip strength (HGS), walking pace (WP), appendicular lean (ALM)] IEU Open GWAS project (461,089 inverse variance weighted method used, MR-Egger, median, MR-PRESSO used sensitivity analyses. Metabolic pathways analysis further performed. Results Fifty-four associated selected 275 pool. Metabolites that are causally linked mainly enriched amino sugar nucleotide metabolism, galactose fructose mannose carnitine synthesis, biotin metabolism. associations pentadecanoate (15:0) ALM, 3-dehydrocarnitine isovalerylcarnitine HGS significant after Bonferroni correction threshold P < 1.82 × 10 − 4 (0.05/275). Meanwhile, hyodeoxycholate glycine right HGS, androsterone sulfate ALM Conclusion Blood different metabolism related These findings might benefit understanding biological mechanisms targeted drugs development health.

Язык: Английский

---

Associations of serum arginine acid with sarcopenia in Chinese eldely women

Nutrition & Metabolism, Год журнала: 2024, Номер 21(1)

Опубликована: Авг. 8, 2024

The prevalence of sarcopenia is increasing in worldwide with accelerated aging process. high dietary protein intakes are associated improved muscle mass and strength especially Asian countries. However, there few researches on amino acid levels or mechanism exploration. We conducted a case-control study to explore the metabolic characteristics potential elderly women using targeted metabolomics approach combined an analysis intake. For our study, we recruited (65–75 y) from Shanghai community 50 patients healthy controls. consensus updated by Working Group Sarcopenia 2019 was used screening for control groups. collected fasting blood samples evaluated acid-targeted ultra performance liquid chromatography tandem spectrometry identify differentials between case groups significantly enriched pathways. (sarcopenia) group had lower intake energy, protein, high-quality (P < 0.05) compared (healthy) group. identified four differential acids: arginine 0.001) cystine = 0.003) were lower, taurine higher Low risk sarcopenia.

Язык: Английский

---

Cross-Species Studies Reveal That Dysregulated Mitochondrial Gene Expression and Electron Transport Complex I Activity Are Crucial for Sarcopenia

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(19), С. 10302 - 10302

Опубликована: Сен. 25, 2024

The significance of complex I the electron transport chain (ETC) in aging process is widely acknowledged; however, its specific impact on development sarcopenia muscle remains poorly understood. This study elucidated correlation between inhibition and by conducting a comparative analysis skeletal gene expression phenotypes from rats, mice, humans. Our findings reveal common mechanistic link across species, particularly highlighting suppression ETC activity dysregulated mitochondrial transcription translation phenotypes. Additionally, we observed macrophage dysfunction alongside abnormal metabolic processes within tissues all implicating their pathogenic role onset sarcopenia. These discoveries underscore importance understanding shared mechanisms associated with development. identified correlations provide valuable insights into potential targets for therapeutic interventions aimed at mitigating sarcopenia, condition substantial implications populations.

Язык: Английский

---

UNLOCKING DIAGNOSIS OF SARCOPENIA: THE ROLE OF CIRCULATING BIOMARKERS - A CLINICAL SYSTEMATIC REVIEW

Mechanisms of Ageing and Development, Год журнала: 2024, Номер 222, С. 112005 - 112005

Опубликована: Ноя. 8, 2024

Язык: Английский

---

Sarcopenia and the biological determinants of aging: A narrative review from a geroscience perspective

Ageing Research Reviews, Год журнала: 2024, Номер 103, С. 102587 - 102587

Опубликована: Ноя. 19, 2024

Язык: Английский

---

Plasma amino acid signature for sarcopenic phenotypes in community-dwelling octogenarians: Results from the Kawasaki Aging Wellbeing Project

Experimental Gerontology, Год журнала: 2023, Номер 178, С. 112230 - 112230

Опубликована: Июнь 6, 2023

Sarcopenia is one of the primary risk factors for various adverse health events in later life. However, its pathophysiology very old population remains unclear. Hence, this study aimed to examine whether plasma free amino acids (PFAAs) correlate with major sarcopenic phenotypes (i.e., muscle mass, strength, and physical performance) community-dwelling adults aged 85-89 years living Japan. Cross-sectional data from Kawasaki Aging Well-being Project were used. We included 133 years. In study, fasting blood was collected measure 20 PFAAs. Measures three appendicular lean mass assessed by multifrequency bioimpedance, isometric handgrip gait speed a 5 m walk at usual pace. Furthermore, we used phenotype-specific elastic net regression models adjusted age centered 85 years, sex, body index, education level, smoking status, drinking habit identify significant PFAAs each phenotype. Higher histidine lower alanine levels associated poor speed, but no correlated strength or mass. conclusion, such as are novel biomarkers performance older.

Язык: Английский

---

Association of serum metabolites with frailty phenotype and its components: a cross-sectional case–control study

Biogerontology, Год журнала: 2024, Номер 26(1)

Опубликована: Дек. 6, 2024

Язык: Английский

---

Plasma biomarkers in patients with age-related sarcopenia: a proteomic exploration and experimental validation

Aging Clinical and Experimental Research, Год журнала: 2024, Номер 37(1)

Опубликована: Дек. 27, 2024

Abstract Background Various biomarkers associated with sarcopenia have been identified. However, there is a scarcity of studies exploring and validating in individuals age-related sarcopenia. Aims This study aimed to investigate the proteome identify potential for Methods Proteomic analysis experimental validation were conducted using plasma from hospitalized older adults. Sarcopenia diagnosis was based on Asian Working Group 2019 criteria. Data-independent acquisition-based proteomics performed 60 participants, 30 diagnosed without Differentially expressed proteins (DEPs) selected evaluated by Receiver Operating Characteristic (ROC) analysis. Biomarker candidates further quantitatively validated enzyme-linked immunosorbent assay (ELISA) utilizing 6 participants Results A total 39 DEPs identified 12 ROC 8 included ELISA their predictive performance. Paraoxonase-3 (PON3) consistently showed down-regulation sarcopenic group across both methodologies. Insulin-like growth factor-binding protein-2 (IGFBP2) inconsistency group, up-regulation observed proteomic but ELISA. Discussion Decline PON3 may result an overload oxidative stress skeletal muscles contribute Protein modifications IGFBP2 might exhibit during pathogenesis. Conclusions Plasma are implicated highlighted as biomarker patients Further imperative gain in-depth understanding IGFBP2.

Язык: Английский

---