Quercetin increases the antidepressant‐like effects of sclareol and antagonizes diazepam in thiopental sodium‐induced sleeping mice: A possible GABAergic transmission intervention

Phytotherapy Research, Год журнала: 2024, Номер 38(5), С. 2198 - 2214

Опубликована: Фев. 27, 2024

Abstract Quercetin is the most common polyphenolic flavonoid present in fruits and vegetables demonstrating versatile health‐promoting effects. This study aimed to examine effects of quercetin (QR) sclareol (SCL) on thiopental sodium (TS)‐induced sleeping forced swimming test (FST) mouse models. SCL (1, 5, 10 mg/kg, p.o .) or QR (50 p.o.) and/or diazepam (DZP) (3 i.p.) were employed. After 30 min TS induction, individual combined animals checked. In FST test, subjected after administration controls for 5 min. this case, immobility time was measured. silico studies conducted evaluate involvement GABA receptors. (5 mg/kg) significantly increased latency decreased compared control TS‐induced study. DZP showed a sedative‐like effect both studies. exhibited similar pattern activity as SCL. However, its more prominent than those groups. (10 altered DZP‐3‐mediated SCL‐10 co‐treated with QR‐50 ( p < 0.05) sleep time, suggesting possible synergistic antidepressant‐like revealed that demonstrated better binding affinities GABAA receptor, especially α 2 , 3 subunits. Both compounds also good ADMET drug‐like properties. animal studies, worked synergistically provide slightly different fashion. As conclusion, may be used upcoming neurological clinical trials, according vivo findings. additional investigation necessary verify behavior clarify potential mechanism action.

Язык: Английский

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Anti-inflammatory activity of d-pinitol possibly through inhibiting COX-2 enzyme: in vivo and in silico studies

Frontiers in Chemistry, Год журнала: 2024, Номер 12

Опубликована: Апрель 16, 2024

Introduction: D-pinitol, a naturally occurring inositol, has diverse biological activities like antioxidant, antimicrobial and anticancer activities. This study aimed to evaluate anti-inflammatory effect of d-pinitol in chick model. Additionally, silico studies were performed the molecular interactions with cyclooxygenase-2 (COX-2). Methods: The tested groups received (12.5, 25, 50 mg/kg) standard drugs celecoxib ketoprofen (42 via oral gavage prior formalin injection. Then, number licks was counted for first 10 min, paw edema diameter measured at 60, 90, 120 min. Results Discussion: significantly ( p &lt; 0.05) reduced diameters, compared negative control. When combined celecoxib, it inflammatory parameters more effectively than individual groups. showed promising binding capacity COX-2. Taken together, exerted effects dose-dependent manner, possibly through COX-2 interaction pathway.

Язык: Английский

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Sclareol exerts an anti-inflammatory effect, possibly through COXs inhibition pathway: In vivo and in silico studies

Pharmaceutical Science Advances, Год журнала: 2023, Номер 2, С. 100029 - 100029

Опубликована: Ноя. 23, 2023

Chronic and severe inflammation results in many diseases disorders humans. Currently, available conventional anti-inflammatory drugs have numerous mild-to-severe side effects. Thus, there is a need for safe, effective, affordable, alternative drugs. This study aimed to evaluate the effect of sclareol (SCL), diterpene alcohol that principal ingredient refined oil Salvia sclarea (L.), through vivo silico studies. For this, we examined individual combined effects SCL (5, 10, 20 mg/kg) standard celecoxib (CXB) or ketoprofen (KPN) at 42 mg/kg (p.o.) on formalin-induced inflammatory Swiss mice. Additionally, an analysis was conducted potential mechanism this study. potentiality standards interact with cyclooxygenase (COX-1 COX-2) receptors. Our findings suggest exhibits dose-dependent SCL-20 significantly reduced number paw licks edema diameters. Moreover, CXN-42 KPN-42 demonstrated better In comparison standards, revealed comparable binding interaction COX-1 COX-2 receptors molecular docking Furthermore, displayed remarkable pharmacokinetic characteristics. conclusion, dose-dependently diameters animals. may be responsible producing by interacting

Язык: Английский

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Anxiolytic-like effect of daidzin possibly through GABAA receptor α2 and α3 subunits interaction pathway: in vivo and in silico studies

Pharmacological Research - Natural Products, Год журнала: 2024, Номер unknown, С. 100090 - 100090

Опубликована: Сен. 1, 2024

Язык: Английский

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Modulatory Anxiolytic Effect of Aucubin on Diazepam in Swiss Albino Mice: Possible Mechanisms Through In Vivo Approach with Receptor Binding Affinity

Revista Brasileira de Farmacognosia, Год журнала: 2025, Номер unknown

Опубликована: Фев. 20, 2025

Язык: Английский

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Organic acids from ice wine ameliorate fructose-induced disorders of glycolipid metabolism in C57BL/6J mice

Food & Function, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Studies have confirmed that long-term consumption of ice wine does not induce disorders glycolipid metabolism, and the high content malic succinic acids in inhibits negative effects fructose to a certain extent.

Язык: Английский

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GABAergic antidepressant effect of daidzin: in vivo approach with in silico receptor binding affinities

In Silico Pharmacology, Год журнала: 2025, Номер 13(1)

Опубликована: Апрель 15, 2025

Язык: Английский

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Antiemetic effects of sclareol, possibly through 5-HT3 and D2 receptor interaction pathways: In-vivo and in-silico studies

Food and Chemical Toxicology, Год журнала: 2023, Номер 181, С. 114068 - 114068

Опубликована: Окт. 18, 2023

Язык: Английский

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Sclareol exerts synergistic antidepressant effects with quercetin and caffeine, possibly suppressing GABAergic transmission in chicks

Biomedicine & Pharmacotherapy, Год журнала: 2023, Номер 168, С. 115768 - 115768

Опубликована: Окт. 20, 2023

This study evaluated the effects of sclareol (SCL) with or without caffeine (CAF) and quercetin (QUR) using in-vivo in-silico studies. For this, 5-day-old chicks weighing between 45 48 g were randomly divided into five groups treated accordingly. The monitored to compare occurrence, latency, duration sleep as well loss gain righting reflex in response SCL-10 mg/kg, CAF-10 QUR-50 mg/kg a thiopental sodium (TS)-induced sleeping model. Data analyzed by one-way ANOVA followed t-Student–Newman-Keuls' posthoc test at 95% confidence intervals multiple comparisons. An was also performed investigate possible antidepressant mechanisms and/or standard drugs different subunits GABAA receptors. In comparison SCL, CAF, QUR individual groups, SCL+CAF+QUR significantly increased latency while decreasing length sleep. incidence modulated combination group. SCL showed better interaction (α2 α5) than α2, α3, α5. All these compounds stronger interactions receptor CAF. Taken together, reduced TS-induced time group more treatments. may show its effects, possibly through subunits.

Язык: Английский

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Anxiolytic Efficacy of Indirubin: In Vivo Approach Along with Receptor Binding Profiling and Molecular Interaction with GABAergic Pathways

ChemistryOpen, Год журнала: 2024, Номер unknown

Опубликована: Окт. 25, 2024

Anxiety is a natural response to stress, characterized by feelings of worry, fear, or unease. The current research was conducted investigate the anxiolytic effect indirubin (IND) in different behavioral paradigms Swiss albino mice. To observe animal's behavioural assess activity, tests were performed, such as open-field (square cross, grooming, and rearing), swing, dark-light, hole cross tests. experimental mice administered IND (5 10 mg/kg, p.o.), where diazepam (DZP) vehicle used positive negative controls, respectively. In addition, combination treatment (DZP+IND-10) provided animals determine modulatory on DZP. Molecular docking approach also binding energy with GABA

Язык: Английский

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