Transcriptomics and microbiome insights reveal the protective mechanism of mulberry-derived postbiotics against inflammation in LPS-induced mice
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 18, 2025
Natural
food-derived
bioactive
compounds
have
garnered
increasing
attention
for
their
potential
to
modulate
immune
responses
and
promote
gut
health.
In
particular,
like
mulberry-derived
postbiotics
(MDP)
may
offer
novel
therapeutic
strategies
address
inflammation,
a
key
driver
of
many
metabolic
disorders.
This
study
examines
the
protective
effects
MDP
against
inflammation
in
LPS-induced
mice,
using
transcriptomic
microbiome
analyses
explore
underlying
mechanisms.
pretreatment
alleviates
LPSinduced
villous
atrophy
intestinal
barrier
damage,
promoting
recovery
morphology.
Transcriptomic
profiling
revealed
significant
changes
gene
expression,
with
983
upregulated
1220
downregulated
genes
NC
vs
LPS
comparison,
380
204
LPS+MDP
comparison.
Enrichment
analysis
GO
KEGG
pathways
associations
transcriptional
regulatory
activity,
NOD-like
receptor
signaling
pathway
among
differentially
expressed
genes.
Protein-protein
interaction
identified
involved
regulation,
hub
IL6,
CXCL10,
MYD88
group
CD74,
CIITA,
H2-AB1
MDP-treated
group.
Microbiome
suggested
also
influence
microbiota
composition,
supporting
systemic
regulation.
These
findings
highlight
MDP's
as
food
additive
modulation
Язык: Английский
Potential Role and Mechanism of Mulberry Extract in Immune Modulation: Focus on Chemical Compositions, Mechanistic Insights, and Extraction Techniques
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(10), С. 5333 - 5333
Опубликована: Май 14, 2024
Mulberry
is
a
rapidly
growing
plant
that
thrives
in
diverse
climatic,
topographical,
and
soil
types,
spanning
temperature
temperate
countries.
plants
are
valued
as
functional
foods
for
their
abundant
chemical
composition,
serving
significant
reservoir
of
bioactive
compounds
like
proteins,
polysaccharides,
phenolics,
flavonoids.
Moreover,
these
displayed
potent
antioxidant
activity
by
scavenging
free
radicals,
inhibiting
reactive
oxygen
species
generation,
restoring
elevated
nitric
oxide
production
induced
LPS
stimulation
through
the
downregulation
inducible
NO
synthase
expression.
Active
components
oxyresveratrol
found
Язык: Английский
Pediococcus pentosaceus JS35 improved flavor, metabolic profile of fermentation supernatant of mulberry leaf powder and increased its antioxidant capacity
Frontiers in Nutrition,
Год журнала:
2025,
Номер
12
Опубликована: Март 4, 2025
Pediococcus
pentosaceus
JS35
was
used
to
improve
flavor,
metabolic
profile
and
antioxidant
activity
of
mulberry
leaf
powder.
Gas
chromatography
ion
mobility
spectrometry
(GC-IMS)
analysis
revealed
that
fermentation
increased
the
contents
floral
fruity
flavor
compounds
such
as
dihydrolinalool
2-phenylethanol,
while
decreased
grassy,
pungent
odor
compounds.
Non-targeted
metabolomics
showed
altered
leaf,
especially
content
flavonoids
metabolites
kaempferol,
quercetin
daidzein.
Compared
with
unfermented
sample,
fermented
supernatant
had
higher
capacity
in
vitro
Caenorhabditis
elegans
.
Furthermore,
supplementation
significantly
prolonged
lifespan
In
conclusion,
by
improved
active
product
effective
capacity.
This
study
will
provide
ideas
for
application
processing
into
functional
foods
or
food
ingredient.
Язык: Английский
Production and characterization of novel antioxidant peptides from mulberry leaf ferment using B. subtilis H4 and B. amyloliquefaciens LFB112
Food Chemistry,
Год журнала:
2025,
Номер
482, С. 144022 - 144022
Опубликована: Апрель 6, 2025
The
objective
of
this
study
was
to
isolate
and
characterize
antioxidant
peptides
from
mulberry
leaves
fermented
with
Bacillus
subtilis
H4
amyloliquefaciens
LFB112.
results
indicated
that
fraction
F4
(<1
kDa)
exhibited
superior
DPPH
activity
compared
the
F1
(>10
kDa),
F2
(3-10
F3
(1-3
fractions,
cytoprotective
effect
against
lipopolysaccharides
(LPS)-induced
oxidative
stress
in
RAW264.7
cells.
Three
novel
peptides,
FRFDP,
RFGG,
GPPLAFGGGP,
were
identified
leaf
ferment,
docking
showed
these
could
form
stable
carbon
(covalent)
hydrogen
bonds
active
sites
Keap1,
thus
regulating
Keap1-Nrf2
pathway
by
blocking
Nrf2
binding
on
Keap1.
These
significantly
upregulated
mRNA
expression
Nrf2,
HO-1,
NQO1,
providing
indirect
evidence
their
potential
enhance
cellular
defense
via
possible
activation
pathway.
Furthermore,
good
ABTS
scavenging
activities,
indicating
as
peptides.
This
offers
valuable
insights
into
integration
developing
ferment
a
functional
food
use
feed
additive.
Язык: Английский
Anti-Inflammatory Effects of Cordyceps Cs-HK1 Fungus Exopolysaccharide on Lipopolysaccharide-Stimulated Macrophages via the TLR4/MyD88/NF-κB Pathway
Nutrients,
Год журнала:
2024,
Номер
16(22), С. 3885 - 3885
Опубликована: Ноя. 14, 2024
Chronic
inflammation
is
a
common
factor
in
the
pathological
processes
of
multiple
human
diseases.
EPS-LM,
an
exopolysaccharide
(EPS)
from
Cordyceps
sinensis
fungus
Cs-HK1,
has
shown
notable
anti-inflammatory
activities
previous
studies.
This
study
aimed
to
investigate
major
signaling
events
mediating
effects
EPS-LM
lipopolysaccharide
(LPS)-stimulated
RAW
264.7
macrophage
cell
culture.
treatment
significantly
reduced
LPS-induced
production
pro-inflammatory
mediators,
including
nitric
oxide
(NO)
and
reactive
oxygen
species
(ROS).
It
also
suppressed
expression
levels
Toll-like
receptor
4
(TLR4)
myeloid
differentiation
primary
response
gene
88
(MyD88),
subsequently
delaying
translocation
nuclear
factor-kappa
B
(NF-κB)
nucleus.
Additionally,
co-immunoprecipitation
(Co-IP)
experiments
demonstrated
that
inhibited
binding
TLR4
MyD88.
The
ability
inhibit
TLR4/MyD88/NF-κB
pathway,
coupled
with
its
capacity
reduce
oxidative
stress,
underscores
multifaceted
effects.
These
render
as
promising
candidate
for
comprehensive
management
various
inflammatory
stress-related
conditions,
protecting
against
damage.
Язык: Английский