Transcriptomics and microbiome insights reveal the protective mechanism of mulberry-derived postbiotics against inflammation in LPS-induced mice

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 18, 2025

Natural food-derived bioactive compounds have garnered increasing attention for their potential to modulate immune responses and promote gut health. In particular, like mulberry-derived postbiotics (MDP) may offer novel therapeutic strategies address inflammation, a key driver of many metabolic disorders. This study examines the protective effects MDP against inflammation in LPS-induced mice, using transcriptomic microbiome analyses explore underlying mechanisms. pretreatment alleviates LPSinduced villous atrophy intestinal barrier damage, promoting recovery morphology. Transcriptomic profiling revealed significant changes gene expression, with 983 upregulated 1220 downregulated genes NC vs LPS comparison, 380 204 LPS+MDP comparison. Enrichment analysis GO KEGG pathways associations transcriptional regulatory activity, NOD-like receptor signaling pathway among differentially expressed genes. Protein-protein interaction identified involved regulation, hub IL6, CXCL10, MYD88 group CD74, CIITA, H2-AB1 MDP-treated group. Microbiome suggested also influence microbiota composition, supporting systemic regulation. These findings highlight MDP's as food additive modulation

Language: Английский

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Potential Role and Mechanism of Mulberry Extract in Immune Modulation: Focus on Chemical Compositions, Mechanistic Insights, and Extraction Techniques

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(10), P. 5333 - 5333

Published: May 14, 2024

Mulberry is a rapidly growing plant that thrives in diverse climatic, topographical, and soil types, spanning temperature temperate countries. plants are valued as functional foods for their abundant chemical composition, serving significant reservoir of bioactive compounds like proteins, polysaccharides, phenolics, flavonoids. Moreover, these displayed potent antioxidant activity by scavenging free radicals, inhibiting reactive oxygen species generation, restoring elevated nitric oxide production induced LPS stimulation through the downregulation inducible NO synthase expression. Active components oxyresveratrol found

Language: Английский

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Pediococcus pentosaceus JS35 improved flavor, metabolic profile of fermentation supernatant of mulberry leaf powder and increased its antioxidant capacity

Frontiers in Nutrition, Journal Year: 2025, Volume and Issue: 12

Published: March 4, 2025

Pediococcus pentosaceus JS35 was used to improve flavor, metabolic profile and antioxidant activity of mulberry leaf powder. Gas chromatography ion mobility spectrometry (GC-IMS) analysis revealed that fermentation increased the contents floral fruity flavor compounds such as dihydrolinalool 2-phenylethanol, while decreased grassy, pungent odor compounds. Non-targeted metabolomics showed altered leaf, especially content flavonoids metabolites kaempferol, quercetin daidzein. Compared with unfermented sample, fermented supernatant had higher capacity in vitro Caenorhabditis elegans . Furthermore, supplementation significantly prolonged lifespan In conclusion, by improved active product effective capacity. This study will provide ideas for application processing into functional foods or food ingredient.

Language: Английский

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Production and characterization of novel antioxidant peptides from mulberry leaf ferment using B. subtilis H4 and B. amyloliquefaciens LFB112

Food Chemistry, Journal Year: 2025, Volume and Issue: 482, P. 144022 - 144022

Published: April 6, 2025

The objective of this study was to isolate and characterize antioxidant peptides from mulberry leaves fermented with Bacillus subtilis H4 amyloliquefaciens LFB112. results indicated that fraction F4 (<1 kDa) exhibited superior DPPH activity compared the F1 (>10 kDa), F2 (3-10 F3 (1-3 fractions, cytoprotective effect against lipopolysaccharides (LPS)-induced oxidative stress in RAW264.7 cells. Three novel peptides, FRFDP, RFGG, GPPLAFGGGP, were identified leaf ferment, docking showed these could form stable carbon (covalent) hydrogen bonds active sites Keap1, thus regulating Keap1-Nrf2 pathway by blocking Nrf2 binding on Keap1. These significantly upregulated mRNA expression Nrf2, HO-1, NQO1, providing indirect evidence their potential enhance cellular defense via possible activation pathway. Furthermore, good ABTS scavenging activities, indicating as peptides. This offers valuable insights into integration developing ferment a functional food use feed additive.

Language: Английский

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Anti-Inflammatory Effects of Cordyceps Cs-HK1 Fungus Exopolysaccharide on Lipopolysaccharide-Stimulated Macrophages via the TLR4/MyD88/NF-κB Pathway

Nutrients, Journal Year: 2024, Volume and Issue: 16(22), P. 3885 - 3885

Published: Nov. 14, 2024

Chronic inflammation is a common factor in the pathological processes of multiple human diseases. EPS-LM, an exopolysaccharide (EPS) from Cordyceps sinensis fungus Cs-HK1, has shown notable anti-inflammatory activities previous studies. This study aimed to investigate major signaling events mediating effects EPS-LM lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cell culture. treatment significantly reduced LPS-induced production pro-inflammatory mediators, including nitric oxide (NO) and reactive oxygen species (ROS). It also suppressed expression levels Toll-like receptor 4 (TLR4) myeloid differentiation primary response gene 88 (MyD88), subsequently delaying translocation nuclear factor-kappa B (NF-κB) nucleus. Additionally, co-immunoprecipitation (Co-IP) experiments demonstrated that inhibited binding TLR4 MyD88. The ability inhibit TLR4/MyD88/NF-κB pathway, coupled with its capacity reduce oxidative stress, underscores multifaceted effects. These render as promising candidate for comprehensive management various inflammatory stress-related conditions, protecting against damage.

Language: Английский

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