PLoS ONE,
Год журнала:
2024,
Номер
19(12), С. e0310910 - e0310910
Опубликована: Дек. 16, 2024
Proteases
as
one
of
the
most
significant
categories
commercial
enzymes,
serve
nowadays
key
ingredients
in
detergent
formulations.
Therefore,
identifying
detergent-compatible
proteases
with
better
properties
is
a
continuous
exercise.
Accordingly,
we
were
interested
recombinant
production
and
characterization
protease
3075
novel
enzyme
from
thermophilic
indigenous
Cohnella
sp.
A01.
The
biochemical
structural
features
probed
by
employing
bioinformatic
methods
vitro
studies.
bioinformatics
analysis
discovered
that
belongs
to
C
56
—
PfpI
superfamily.
gene
was
cloned
heterologous
expressed
Escherichia
coli
(E
.
coli)
BL21.
It
found
contains
175
amino
acids
525
bp
molecular
weight
19
kDa.
Protease
revealed
acceptable
activity
range
40–80°C
pH
5.5–8.
optimum
observed
at
70°C
6.
increased
about
4-fold
presence
Tween
80
acetone,
while
its
attenuated
iodoacetic
acid
iodoacetamide.
Docking
analyses
dominant
interaction
between
3075,
mediated
hydrogen
bonds
Van
der
Waals
forces.
Furthermore,
dynamics
simulations
(MDS)
showed
stability
structure.
Altogether,
our
data
provided
genetic
manipulation
process
could
have
industrial
applications.
Future Foods,
Год журнала:
2024,
Номер
10, С. 100404 - 100404
Опубликована: Июнь 21, 2024
The
human
food
security
is
unavoidably
dependent
on
exploring
novel
nutritional
sources,
such
as
edible
insects.
However,
insects
farming
needs
to
improve
their
rearing
practices,
extracting
value
from
all
growing
stages,
larvae
until
adults.
In
this
work,
a
full
characterization
of
larvae,
pupae
and
adults
Tenebrio
molitor
was
carried
out.
Higher
protein
ash
contents
were
observed
in
pupae,
while
higher
lipids
(mainly
polyunsaturated
fatty
acids,
PUFA)
decreasing
reaching
the
adult
stage.
Chitin
content
6-fold
than
pupae.
Also,
it
possible
quantify
chitin
resorting
computed
3D
tomography,
achieving
similar
values
traditional
chemically
based
method.
Lower
mineral
found
high
iron,
zinc
copper
levels
beetles.
Phenolic
compounds
among
FTIR
analysis
valuable,
easy,
fast
method
assess
composition
T.
molitor,
detecting
vibrations
characteristic
protein,
fractions.
This
study
provides
life
stages
highlighting
valorisation
chains
that
can
be
adopted
obtain
highly
nutritive
and/or
functional
compounds.
Food and Bioprocess Technology,
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 13, 2024
Abstract
Insects
are
promising
alternatives
to
meet
the
world
population’s
demand
for
high-quality
foods
and
overcome
important
issues
in
animal
farming
practices.
Novel
green
biorefinery
processes
must
be
applied
insects,
overcoming
chemically
based
techniques.
Eco-friendly
approaches
increase
nutritional
value
of
widening
commercial
applications.
This
review
addresses
rearing
practices
composition
Tenebrio
molitor
,
highlighting
methodologies
that
can
obtain
value-added
compounds,
replacing
unsustainable
Also,
useful
applications
pre-treated
T.
biomass
presented
with
a
thoughtful
insight
into
their
advantages
limitations.
The
richness
is
being
successfully
explored
by
resorting
physical
biological
procedures,
resulting
valuable
compounds
food,
feeding,
biomedical
biotechnological
industries.
ingredients
additives
insect
origin
may
upgrade
food
feed
formulation,
while
chitosan
packaging
industries
feed.
