Regulation of calcium homeostasis in endoplasmic reticulum–mitochondria crosstalk: implications for skeletal muscle atrophy

Cell Communication and Signaling, Год журнала: 2025, Номер 23(1)

Опубликована: Янв. 9, 2025

This review comprehensively explores the critical role of calcium as an essential small-molecule biomessenger in skeletal muscle function. Calcium is vital for both regulating excitation–contraction coupling and development, maintenance, regeneration cells. The orchestrated release from endoplasmic reticulum (ER) mediated by receptors such ryanodine receptor (RYR) inositol 1,4,5-trisphosphate (IP3R), which crucial contraction. sarcoendoplasmic ATPase (SERCA) pump plays a key recapturing calcium, enabling to return relaxed state. A pivotal aspect homeostasis involves dynamic interaction between mitochondria ER. includes local signaling facilitated RYRs "quasi-synaptic" mechanism formed IP3R-Grp75-VDAC/MCU axis, allowing rapid uptake with minimal interference at cytoplasmic level. Disruption transport can lead mitochondrial overload, triggering opening permeability transition pore subsequent reactive oxygen species cytochrome C, ultimately resulting damage atrophy. complex relationship ER how these organelles regulate levels muscle, aiming provide valuable perspectives future research on pathogenesis diseases development prevention strategies.

Язык: Английский

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The correlation between mitochondria-associated endoplasmic reticulum membranes (MAMs) and Ca2+ transport in the pathogenesis of diseases

Acta Pharmacologica Sinica, Год журнала: 2024, Номер unknown

Опубликована: Авг. 8, 2024

Язык: Английский

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Stigmasterol from Prunella vulgaris L. Alleviates LPS-Induced Mammary Gland Injury by Inhibiting Inflammation and Ferroptosis

Phytomedicine, Год журнала: 2025, Номер 137, С. 156362 - 156362

Опубликована: Янв. 5, 2025

Язык: Английский

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Mechanism of Ca2+ overload caused by STIM1/ORAI1 activation of store-operated Ca2+ entry (SOCE) in hydrogen peroxide-induced mitochondrial damage and apoptosis in human primary melanocytes

Molecular Biology Reports, Год журнала: 2025, Номер 52(1)

Опубликована: Фев. 12, 2025

Язык: Английский

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Kaempferol regulating macrophage foaming and atherosclerosis through piezo1-mediated MAPK/NF-κB and Nrf2/HO-1 signaling pathway

Journal of Advanced Research, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

Antioxidants represented by kaempferol have been shown to be effective against atherosclerosis (AS). However, the underlying mechanisms still remain unclear.

Язык: Английский

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Amelioration Effects and Regulatory Mechanisms of Different Tea Active Ingredients on DSS-Induced Colitis

Journal of Agricultural and Food Chemistry, Год журнала: 2023, Номер 71(44), С. 16604 - 16617

Опубликована: Окт. 25, 2023

The potential biological function of tea and its active components on colitis has attracted wide attention. In this study, different ingredients including polyphenols (TPPs), polysaccharides (TPSs), theabrownin (TB), theanine (TA) have been compared in the intervention dextran sulfate sodium (DSS)-induced mice. Specifically, TPP showed greatest effect since it reduced 60.87% disease activity index (DAI) to that DSS-induced group, followed by reduction 39.13% TPS 28.26% TB DAI, whereas there was no obvious alleviative TA colitis. TPP, TPS, could regulate composition abundance gut microbiota increase content short-chain fatty acids (SCFAs) enhance intestinal barrier function. Further evidence observed regulated activation Nrf2/ARE TLR4/MyD88/NF-κB P65 pathway alleviate Results cell experiments demonstrated antiapoptosis mitochondrial protective capability among via inhibiting Cytc/Cleaved-caspase-3 signaling pathway. summary, superior anticolitis is primarily attributed unique upregulation Akkermansia ability

Язык: Английский

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STIM2 variants regulate Orai1/TRPC1/TRPC4-mediated store-operated Ca2+ entry and mitochondrial Ca2+ homeostasis in cardiomyocytes

Cell Calcium, Год журнала: 2024, Номер 119, С. 102871 - 102871

Опубликована: Март 19, 2024

The stromal interaction molecules (STIMs) are the sarcoplasmic reticulum (SR) Ca2+ sensors that trigger store-operated entry (SOCE) in a variety of cell types. While STIM1 isoform has been focus research cardiac pathophysiology, function homolog STIM2 remains unknown. Using imaging and patch-clamp techniques, we showed knockdown (KD) by siRNAs increased SOCE ISOC current neonatal rat ventricular cardiomyocytes (NRVMs). Within this cardiomyocyte model, identified transcript expression Stim2.1 Stim2.2 splice variants, with predominance for Stim2.2. conventional super-resolution confocal microscopy (STED), found exogenous STIM2.1 STIM2.2 formed pre-clusters reticular organization at rest. Following SR store depletion, some clusters were translocated to SR-plasma membrane (PM) junctions co-localized Orai1. overexpression strategy revealed suppressed Orai1-mediated while enhanced SOCE. STIM2.2-enhanced was also dependent on TRPC1 TRPC4. Even if KD or variants did not affect cytosolic cycling, observed, using Rhod-2/AM imaging, Orai1 inhibition abolished mitochondrial (mCa2+) uptake, as opposed KD. We present mitochondria-associated endoplasmic (ER) membranes (MAMs) interacting inositol trisphosphate receptors (IP3Rs), voltage-dependent anion channel (VDAC), uniporter (MCU), mitofusin-2 (MNF2). Our results suggested that, NRVMs, constitutes predominant functional variant negatively regulates Orai1-generated It participates control mCa2+ uptake capacity possibly via STIM2-IP3Rs-VDAC-MCU MNF2 complex.

Язык: Английский

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Exploring the impact of cationic polysaccharide concentration on the formation of high internal phase emulsion based on egg white proteins: A study on emulsion interfacial structure and co-delivery of epigallocatechin gallate/β-carotene

Food Hydrocolloids, Год журнала: 2025, Номер unknown, С. 111250 - 111250

Опубликована: Фев. 1, 2025

Язык: Английский

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Dietary carotenoid intakes and biological aging among US adults, NHANES 1999–2018

Nutrition Journal, Год журнала: 2025, Номер 24(1)

Опубликована: Янв. 16, 2025

Язык: Английский

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l-Glutamic Acid Alleviates Mastitis in Dairy Cows by Targeting the Sirt5/Nrf2/Prdx1 Signaling Axis and Mitochondrial Function

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 2, 2025

Mastitis in dairy cows is an inflammatory disease that severely affects the health and lactation functions of cows. Mitochondrial damage closely related to response. How effectively alleviate mitochondrial key preventing treating mastitis In this study, we found elevated levels response accompanied by reduced expression Sirt5 (Sirtuin5) with compared healthy This suggests plays important role Subsequently, further analyzed mammary gland tissue from untargeted metabolomics (LC-MS/MS) screened for differential metabolite l-glutamic acid (l-Glu). To validate effect l-Glu on cows, conducted a study using MAC-T cells. The results showed was able ameliorate LPS-induced activating promoting fusion upregulation membrane potential (MMP) levels. contrast, unable protect function after knocking down Sirt5. Furthermore, upregulate nuclear factor E2-related (Nrf2) peroxiredoxin 1 (Prdx1) cells, promoted entry Nrf2 into nucleus, which reversed Next, explored whether alleviates through Nrf2/Prdx1 signaling axis inhibitor RA. use RA blocked protective function. conclusion, ameliorates targeting activate provides new target theoretical basis clinical control mastitis. could be added as dietary supplement diets maintain homeostasis, thereby protecting economic value

Язык: Английский

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