Redox Biology,
Год журнала:
2024,
Номер
70, С. 103063 - 103063
Опубликована: Фев. 2, 2024
Vascular
diseases,
a
leading
cause
of
death
in
human,
are
strongly
associated
with
pathological
damage
to
blood
vessels.
The
selenoprotein
(Sel)
have
been
reported
play
important
roles
vascular
disease.
However,
the
role
SelO
disease
has
not
conclusively
investigated.
present
experiment
was
investigate
regulatory
mechanism
effect
on
permeability
endothelial.
H.E
staining,
FITC-Dextran
Dil-AC-LDL
staining
and
FITC-WGA
showed
that
structure
damaged,
intercellular
junctions
were
disrupted
selenium
(Se)-deficient.
Immunohistochemistry,
qPCR
Western
blot
revealed
decreased
expression
adhesion
plaque
proteins
vinculin,
talin
paxillin,
connectivity
effector
molecules
connexin,
claudin-1
E-cadherin
increased
JAM-A
N-cadherin,
as
well
ZO-1
signaling
pathways
ZO-1,
Rock,
rhoGEF,
cingulin
MLC-2.
In
screening
24
Sel
mice,
most
pronounced
changes
tissues,
possible
association
between
junction
effectors
determined
using
IBM
SPSS
Statistics
25.
Silencing
SelO,
endothelial
adverse
effects
present.
relationship
determined.
results
Se
deficiency
lead
tissue
by
decreasing
expression,
suggesting
for
regulating
permeability.
Microbiology Spectrum,
Год журнала:
2024,
Номер
12(4)
Опубликована: Фев. 20, 2024
ABSTRACT
Given
the
burgeoning
Nyctereutes
procyonoides
breeding
industry
and
its
growing
scale,
it
is
imperative
to
investigate
impact
of
high-fat
diets
on
health
these
animals.
This
study
involved
30
male
comparable
weights
(3
kg
±0.5),
randomly
assigned
either
a
control
group
or
diet
(
n
=
15
each).
The
latter
was
fed
mixture
lard
basal
in
2:5
ratio,
establishing
model
procyonoides.
induced
diarrhea
histopathological
changes
Analysis
small
intestine
contents
using
16S
rRNA
sequencing
revealed
diet-induced
disruption
gut
microbiota.
Specifically,
Escherichia-Shigella
emerged
as
biomarker
P
0.049),
while
Vagococcus
prevalent
indicating
significant
increase
harmful
bacteria
group.
Furthermore,
this
disrupted
flora
correlated
with
inflammation
oxidative
stress,
evidenced
by
marked
increases
TNF-α
<
0.01),
IL-1β
0.05),
IL-6
0.05)
levels,
measured
via
q-PCR,
Western
blot,
stress
assays.
In
addition,
q-PCR
analysis
upregulation
apoptosis
necrosis
markers,
including
Bax,
Caspase3,
Caspase9,
Caspase12,
RIPK3,
RIPK1
0.01
0.001),
concurrent
downregulation
anti-apoptotic
gene
Bcl-2
0.01)
group,
consistent
protein
expression
trends.
These
findings
suggest
that
alters
microbiome
toward
more
bacterial
composition,
escalating
inflammatory
responses
intestinal
tissue
permeability,
culminating
cell
necrosis.
IMPORTANCE
examines
Our
research
established
diet,
revealing
impacts,
such
diarrhea,
histological
anomalies,
alterations
emphasize
importance
preventing
issues
promoting
sustainable
growth.
They
highlight
microbiota
overall
animal
health.
Food & Function,
Год журнала:
2024,
Номер
15(7), С. 3411 - 3419
Опубликована: Янв. 1, 2024
Tetrabromobisphenol
A
(TBBPA)
is
a
global
pollutant.
When
TBBPA
absorbed
by
the
body
through
various
routes,
it
can
have
wide
range
of
harmful
effects
on
body.
Green
tea
polyphenols
(GTPs)
act
as
antioxidants,
resisting
toxic
animals.
The
and
mechanisms
GTP
oxidative
stress,
inflammation
apoptosis
in
mouse
lung
are
unknown.
Therefore,
we
established
vivo
vitro
models
exposure
antagonism
using
C57
mice
A549
cells
examined
expression
factors
related
to
autophagy,
apoptosis.
results
study
showed
that
increase
reactive
oxygen
species
(ROS)
levels
after
decreased
autophagy-related
Beclin1,
LC3-II,
ATG3,
ATG5,
ATG7
ATG12
increased
p62;
stress
inhibits
autophagy
levels.
pro-inflammatory
IL-1β,
IL-6
TNF-α
anti-inflammatory
factor
IL-10
activation
NF-κB
p65/TNF-α
pathway.
Bax,
caspase-3,
caspase-7
caspase-9
Bcl-2
activate
apoptosis-related
pathways.
addition
attenuated
levels,
restored
inhibition
reduced
Our
suggest
attenuate
modulating
ROS,
reducing
increasing
attenuating
cells.
These
provide
fundamental
information
for
exploring
antioxidant
mechanism
further
studying
TBBPA.
Food & Function,
Год журнала:
2024,
Номер
15(13), С. 7136 - 7147
Опубликована: Янв. 1, 2024
Zinc
(Zn)
is
an
important
trace
element;
it
involved
in
the
regulation
and
maintenance
of
many
physiological
functions
organisms
has
anti-inflammatory
antioxidant
properties.
Animals,
Год журнала:
2025,
Номер
15(3), С. 387 - 387
Опубликована: Янв. 30, 2025
Tea
polyphenol
(TP)
is
a
kind
of
natural
macromolecular
compound
present
in
tea
extract
with
rich
biological
potential.
The
purpose
this
study
was
to
explore
the
protective
effect
TP
on
intestinal
immune
barrier
and
related
mechanisms
alleviating
injury.
Models
common
carp
(Cyprinus
carpio
L.)
primary
epithelial
cells
treated
lipopolysaccharide
(LPS)
were
established.
qPCR
ELISA
results
showed
that
increased
levels
lysozyme
(LZ),
alkaline
phosphatase
(ALP),
complement
component
3
(C3),
4
(C4),
immunoglobulin
T
(IgT),
D
(IgD),
M
(IgM)
activate
ability.
Molecular
docking
indicated
had
strong
interaction
TLR2.
Meanwhile,
alleviated
LPS-induced
inflammatory
damage
as
evidenced
by
reducing
mRNA
TNF-α,
IL-6,
IL-1β,
TLR2,
MyD88,
P65,
IκBα,
which
consistent
those
Western
blotting
results.
Moreover,
revealed
promoted
tight
junction-related
proteins
(claudins,
occludin,
ZOs)
inhibited
phosphorylation
MLC,
opposite
trend
after
LPS
treatment.
In
summary,
improved
ability
activation
TLR2/NF-κB/MLCK
pathway
attenuate
injury
intestines
carp.
Animals,
Год журнала:
2025,
Номер
15(4), С. 467 - 467
Опубликована: Фев. 7, 2025
In
this
present
study,
we
aimed
to
investigate
the
effects
of
adding
tea
polyphenols
feed
on
immunity,
antioxidant
capacity,
and
gut
microbiota
weaned
goat
kids.
Thirty
kids
(Leizhou
goats,
average
initial
weight
9.32
±
1.72
kg,
2
months
old)
were
randomly
divided
into
five
groups
with
six
in
each
group,
half
being
male
female.
The
control
(CON)
group
was
fed
basal
diet,
four
other
supplemented
2,
4,
or
6
g/kg
50
mg/kg
chlortetracycline
diet
(denoted
as
T1,
T2,
T3,
CTC
groups,
respectively).
results
showed
that
compared
CON
4
could
increase
expression
levels
serum
enzymes
intestinal
genes
It
also
increased
Nrf2
IL-10
intestine,
while
reducing
content
gene
cytokines
(IL-1β,
IL-6,
IFN-γ,
TNF-α).
Dietary
supplementation
reduced
TLR4,
MyD88,
NFκB
tissue,
activated
protective
mechanisms,
enhanced
immune
defense
epithelium.
Compared
feeding
significantly
Simpson
indices.
However,
relative
abundance
Verrucomicrobiota,
Candidatus
Soleaferrea,
Christensenellaceae
R-7
Prevotella,
well
acetic
acid
cecum
(p
<
0.05).
Overall,
indicate
dietary
can
effectively
maintain
homeostasis
enhance
anti-inflammatory
capabilities
Animals,
Год журнала:
2025,
Номер
15(4), С. 489 - 489
Опубликована: Фев. 9, 2025
Zearalenone
(ZEA)
is
a
kind
of
mycotoxin
that
widely
contaminates
food
and
feed
poses
threat
to
poultry
farming.
As
natural
extract,
glycyrrhizin
acid
(GA)
has
antioxidant,
antibacterial,
anti-inflammatory
effects.
Although
studies
have
revealed
the
toxic
effects
ZEA
on
liver,
mechanism
by
which
GA
reduces
ZEA's
protective
glandular
stomach
remains
unclear.
In
order
study
therapeutic
effect
tissue
damage
caused
ZEA,
we
conducted
in
vivo
vitro
experiments
compare
expression
inflammation,
oxidative
stress,
apoptosis,
necrosis.
The
results
showed
can
induce
inflammation
tissues
cells,
inducing
apoptosis
addition,
alleviate
protect
cells.
Dietary
significantly
increased
antioxidant
capacity
glandulae
inhibited
overexpression
NFκB/IκB-α
its
mediated
inflammatory
response.
Moreover,
decreased
pro-apoptotic
factors
necrosis
factors,
thereby
alleviating
chicken
At
present,
livers
lungs
been
confirmed
studies.
However,
there
no
stomach.
Therefore,
purpose
this
was
explore
through
experimental
comparison.
may
provide
some
reference
for
solution
contamination.
