Journal of Biological Chemistry,
Год журнала:
2024,
Номер
unknown, С. 108127 - 108127
Опубликована: Дек. 1, 2024
2-O-α-Glucosylglycerol
(GG)
is
a
natural
heteroside
synthesized
by
many
cyanobacteria
and
few
heterotrophic
bacteria
under
salt
stress
conditions.
Bacteria
produce
GG
in
response
to
stimuli
degrade
it
once
the
stimulus
diminishes.
Heterotrophic
utilize
phosphorylase
(GGP),
member
of
GH13_18
family,
via
two-step
process
consisting
phosphorolysis
hydrolysis
for
catabolism.
However,
precise
mechanism
which
GGP
degrades
remains
elusive.
We
determined
3D
structure
recently
identified
(MsGGP)
deep-sea
bacterium
Marinobacter
salinexigens
ZYF650
Molecules,
Год журнала:
2024,
Номер
29(10), С. 2275 - 2275
Опубликована: Май 11, 2024
The
hydrolysis
and
biotransformation
of
lignocellulose,
i.e.,
biorefinery,
can
provide
human
beings
with
biofuels,
bio-based
chemicals,
materials,
is
an
important
technology
to
solve
the
fossil
energy
crisis
promote
global
sustainable
development.
Biorefinery
involves
steps
such
as
pretreatment,
saccharification,
fermentation,
researchers
have
developed
a
variety
biorefinery
strategies
optimize
process
reduce
costs
in
recent
years.
Lignocellulosic
hydrolysates
are
platforms
that
connect
saccharification
downstream
fermentation.
hydrolysate
composition
closely
related
biomass
raw
pretreatment
process,
choice
biorefining
strategies,
provides
not
only
nutrients
but
also
possible
inhibitors
for
In
this
review,
we
summarized
effects
each
stage
lignocellulosic
on
inhibitors,
analyzed
huge
differences
nutrient
retention
inhibitor
generation
among
various
emphasized
all
lignocellulose
need
be
considered
comprehensively
achieve
maximum
optimal
control
at
low
cost,
reference
development
chemicals.
Abstract
Benefiting
from
the
high
sensitivity
and
electromechanical
conversion
efficiency,
triboelectric
nanogenerators
(TENGs)
are
widely
used
in
various
fields
of
self‐powered
sensing
mechanical
energy
harvesting,
which
have
great
potential
for
application
future
smart
Internet
Things.
The
development
sustainable
materials
with
high‐performance
has
a
vital
impact
on
construction
TENG
devices
that
combine
high‐output
performance
environmental
friendliness,
positive
humanity.
This
review
systematically
comprehensively
summarizes
latest
research
work
TENG's
materials.
First,
an
overall
overview
is
provided
based
composition
materials,
including
amino
acids,
polysaccharides,
synthetic
polymer,
representative
works
further
classified
summarized
detail.
In
addition,
progress
harvesting
applications
also
summarized.
Finally,
overviews
challenges
current
material,
related
outlooks
offered
corresponding
strategies
directions
this
field
future.
Green Carbon,
Год журнала:
2024,
Номер
2(2), С. 242 - 251
Опубликована: Апрель 5, 2024
Methanol,
produced
from
carbon
dioxide,
natural
gas,
and
biomass,
has
drawn
increasing
attention
as
a
promising
green
feedstock
for
biomanufacturing
due
to
its
sustainable
energy-rich
properties.
NAD+-dependent
methanol
dehydrogenase
(MDH)
catalyzes
the
oxidation
of
formaldehyde
via
NADH
generation,
providing
highly
active
C1
intermediate
reducing
power
subsequent
biosynthesis.
However,
unsatisfactory
catalytic
efficiency
cofactor
bias
MDH
significantly
impede
valorization,
especially
in
NADPH-dependent
Herein,
we
employed
synthetic
NADPH
auxotrophic
Escherichia
coli
strains
growth-coupled
selection
platforms
directed
evolution
Bacillus
stearothermophilus
DSM
2334.
or
generated
by
MDH-catalyzed
enabled
growth
auxotrophs,
establishing
positive
correlation
between
cell
rate
activity.
Using
this
principle,
mutants
exhibiting
20-fold
improvement
(kcat/Km)
90-fold
specificity
switch
NAD+
NADP+
without
decrease
specific
enzyme
activity,
were
efficiently
screened
random
semi-rationally
designed
libraries.
We
envision
that
these
will
advance
valorization
auxotrophs
serve
versatile
NAD(P)+-dependent
enzymes.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Окт. 9, 2024
As
an
abundant
agricultural
and
forestry
biomass
resource,
hemicelluloses
are
hard
to
be
effectively
degraded
utilized
by
microorganisms
due
the
constraints
of
membrane
metabolic
regulations.
Herein,
we
report
a
synthetic
extracellular
pathway
with
hemicellulose-degrading-enzymes
controllably
displayed
on
Escherichia
coli
surface
as
engineered
bacterial
consortia
members
for
efficient
utilization
xylan,
most
component
in
hemicellulose.
Further,
develop
hemicellulose/O
Abstract
Lignocellulolytic
clostridia
employ
multiple
pairs
of
alternative
σ/anti‐σ
(SigI/RsgI)
factors
to
regulate
cellulosomal
components
for
substrate‐specific
degradation
cellulosic
biomass.
The
current
model
has
proposed
that
RsgIs
use
a
sensor
domain
bind
specific
extracellular
lignocellulosic
and
activate
cognate
SigIs
initiate
expression
corresponding
enzyme
genes,
while
scaffoldins
can
be
initiated
by
several
different
SigIs.
Pseudobacteroides
cellulosolvens
contains
the
most
complex
known
cellulosome
system
highest
number
SigI–RsgI
regulons
yet
discovered.
However,
function
many
RsgI
domains
their
relationship
with
various
types
are
not
fully
understood.
Here,
we
report
RsgI4
from
P.
employs
C‐terminal
module
bears
distant
similarity
fibronectin
type
III
(Fn3)
serves
as
domain.
Substrate‐binding
analysis
revealed
Fn3‐like
represents
novel
carbohydrate‐binding
(CBM)
binds
wide
range
polysaccharide
types.
Structure
determination
further
belongs
B
group
CBMs
predicted
concave
face
substrate
binding.
Promoter
sequence
genes
SigI4
is
responsible
regulation
major
rather
than
enzymes,
consistent
broad
specificity
Notably,
invariably
required
regardless
type.
These
findings
suggest
intricate
comprises
more
elaborate
mechanism
other
bacteria
thus
expands
paradigm
regulation.
